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Literature summary extracted from

  • Yang, M.; Jensen, R.E.; Yaffe, M.P.; Oppliger, W.; Schatz, G.
    Import of proteins into yeast mitochondria: the purified matrix processing protease contains two subunits which are encoded by the nuclear MAS1 and MAS2 genes (1988), EMBO J., 7, 3857-3862.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.4.24.64 Separation of the loosely associated subunits of MW 48000 and 51000 causes complete loss of activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.64 1,10-phenanthroline
-
 Saccharomyces cerevisiae
3.4.24.64 additional information PMSF Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.64 mitochondrion matrix Saccharomyces cerevisiae 5739
-
3.4.24.64 soluble
-
 Saccharomyces cerevisiae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.64 48000
-
 1 * 48000 (MAS1) + 1 * 51000 (MAS2), Saccharomyces cerevisiae, SDS-PAGE Saccharomyces cerevisiae
3.4.24.64 50000
-
 gel filtration Saccharomyces cerevisiae
3.4.24.64 51000
-
 1 * 48000 (MAS1) + 1 * 51000 (MAS2), Saccharomyces cerevisiae, SDS-PAGE Saccharomyces cerevisiae
3.4.24.64 100000
-
 sucrose density gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.64 additional information Saccharomyces cerevisiae removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes ?
-
 ?
3.4.24.64 additional information Saccharomyces cerevisiae D-273-10B removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.64 Saccharomyces cerevisiae
-
-
-
3.4.24.64 Saccharomyces cerevisiae D-273-10B
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.64 from soluble matrix fraction of isolated mitochondria Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.64 Release of N-terminal targetting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2 cleaves amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins Saccharomyces cerevisiae
a

Storage Stability

EC Number Storage Stability Organism
3.4.24.64 -70°C, after quick-freezing in liquid nitrogen, at least 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.64 Cytochrome b2 precursor + H2O
-
 Saccharomyces cerevisiae Cytochrome b2 intermediate form
-
 ?
3.4.24.64 Cytochrome b2 precursor + H2O
-
 Saccharomyces cerevisiae D-273-10B Cytochrome b2 intermediate form
-
 ?
3.4.24.64 Cytochrome c oxidase subunit IV precursor + H2O
-
 Saccharomyces cerevisiae Cytochrome c oxidase subunit IV
-
 ?
3.4.24.64 Cytochrome c oxidase subunit IV precursor + H2O
-
 Saccharomyces cerevisiae D-273-10B Cytochrome c oxidase subunit IV
-
 ?
3.4.24.64 Cytochrome c1 precursor + H2O membrane-bound enzyme Saccharomyces cerevisiae Cytochrome c1 intermediate form
-
 ?
3.4.24.64 F1-ATPase beta-subunit precursor + H2O
-
 Saccharomyces cerevisiae F1-ATPase beta-subunit
-
 ?
3.4.24.64 F1-ATPase beta-subunit precursor + H2O
-
 Saccharomyces cerevisiae D-273-10B F1-ATPase beta-subunit
-
 ?
3.4.24.64 Mitochondrial alcohol dehydrogenase precursor + H2O
-
 Saccharomyces cerevisiae Mitochondrial alcohol dehydrogenase
-
 ?
3.4.24.64 additional information the large subunit alone has no cleavage activity Saccharomyces cerevisiae ?
-
 ?
3.4.24.64 additional information removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes Saccharomyces cerevisiae ?
-
 ?
3.4.24.64 additional information the large subunit alone has no cleavage activity Saccharomyces cerevisiae D-273-10B ?
-
 ?
3.4.24.64 additional information removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes Saccharomyces cerevisiae D-273-10B ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.64 dimer 1 * 48000 (MAS1) + 1 * 51000 (MAS2), Saccharomyces cerevisiae, SDS-PAGE Saccharomyces cerevisiae
3.4.24.64 More Saccharomyces cerevisiae has two loosely associated non-identical subunits that dissociate upon gel filtration Saccharomyces cerevisiae
3.4.24.64 More the large subunit alone has no cleavage activity Saccharomyces cerevisiae