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Literature summary extracted from

  • Clamagirand, C.; Creminon, C.; Fahy, C.; Boussetta, H.; Cohen, P.
    Partial purification and functional properties of an endoprotease from bovine neurosecretory granules cleaving proocytocin/neurophysin peptides at the basic amino acid doublet (1987), Biochemistry, 26, 6018-6023.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.62 Ca2+ weak, 1 mM, peptide I as substrate Bos taurus
3.4.24.62 Cd2+ weak, 0.1 mM, peptide I as substrate Bos taurus
3.4.24.62 EDTA peptide I as substrate; strong, 2.5 mM Bos taurus
3.4.24.62 EGTA peptide I as substrate; strong, 2.5 mM Bos taurus
3.4.24.62 additional information Mn2+, Mg2+, Cu2+; no inhibition by PMSF, pepstatin Bos taurus
3.4.24.62 p-chloromercuriphenylsulfonic acid 2.5 mM; peptide I as substrate Bos taurus
3.4.24.62 PCMB 2.5 mM; peptide I as substrate Bos taurus
3.4.24.62 Zn2+ 1 mM, peptide I as substrate Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.62 additional information metalloendopeptidase Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.62 52000 58000 bovine, gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.62 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.62 from purified vesicles Bos taurus
3.4.24.62 partial Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.62 hypophysis pituitary neurosecretory granule Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.62 additional information No substrates are Nle11 or Nle12 Bos taurus ?
-
 ?
3.4.24.62 additional information No substrates are D-Lys11 Bos taurus ?
-
 ?
3.4.24.62 additional information No substrates are pro-oxytocin/neurophysin peptides (i.e. pro-OT/Np(1-20)) with D-Arg12 Bos taurus ?
-
 ?
3.4.24.62 Pro-oxytocin/neurophysin(1-20) + H2O synthetic eicosapeptide reproducing the entire sequence of amino-terminal domain of bovine proOc/Np precursor Bos taurus Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
 ?
3.4.24.62 Pro-oxytocin/neurophysin(1-20) + H2O strictly selective for basic amino acid doublet Lys11-Arg12, cleavage site: Arg12-Ala13 Bos taurus Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
 ?
3.4.24.62 Pro-oxytocin/neurophysin(1-20) + H2O i.e. model substrate peptide I Bos taurus Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
 ?
3.4.24.62 [Ala10]-Pro-oxytocin/neurophysin(1-20) + H2O i.e. peptide XI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20) Bos taurus ?
-
 ?
3.4.24.62 [Arg11,Lys12]-Pro-oxytocin/neurophysin(1-20) + H2O i.e. peptide VI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20) Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.62 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.62 additional information
-
 pI: 6.9 and 7.2 (2 isoforms) Bos taurus
3.4.24.62 7
-
 around, peptide I as substrate Bos taurus