Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cohen, P.; Pierotti, A.R.; Chesneau, V.; Foulon, T.; Prat, A.
    N-arginine dibasic convertase (1995), Methods Enzymol., 248, 703-716.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.61 rat, testis Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.61 1,10-phenanthroline 0.1 mM Rattus norvegicus
3.4.24.61 amastatin 0.02 mM Rattus norvegicus
3.4.24.61 bestatin 0.02 mM Rattus norvegicus
3.4.24.61 Ca2+ not Rattus norvegicus
3.4.24.61 Co2+ 0.1 mM Rattus norvegicus
3.4.24.61 Cu2+ 0.1 mM Rattus norvegicus
3.4.24.61 DTT weak Rattus norvegicus
3.4.24.61 EDTA 0.01 mM; not by Cu2+; reversible by low concentrations of Zn2+ or Mn2+; to a lesser extent by Co2+, Ca2+; to a lesser extent by Mg2+ (not) Rattus norvegicus
3.4.24.61 EGTA 0.01 mM Rattus norvegicus
3.4.24.61 Mn2+
-
 Rattus norvegicus
3.4.24.61 additional information Mg2+ (0.1 mM); no inhibition by IAA, phosphoramidon, captopril, phenylmethylsulfonyl fluoride, leupeptin, guanidylethylmercaptosuccinic acid (i.e. GEMSA) Rattus norvegicus
3.4.24.61 N-ethylmaleimide
-
 Rattus norvegicus
3.4.24.61 N-tosyl-L-Phe chloromethyl ketone
-
 Rattus norvegicus
3.4.24.61 Zn2+ 0.1 mM; strong Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.61 0.00625
-
 ANF-(1-28)
-
 Rattus norvegicus
3.4.24.61 0.00645
-
 dynorphin A
-
 Rattus norvegicus
3.4.24.61 0.0173
-
 Preproneurotensin-(154-170)
-
 Rattus norvegicus
3.4.24.61 0.024
-
 dynorphin A-(1-13)
-
 Rattus norvegicus
3.4.24.61 0.043
-
 Somatostatin-28
-
 Rattus norvegicus
3.4.24.61 0.103
-
 Bovine adrenal medulla dodecapeptide
-
 Rattus norvegicus
3.4.24.61 0.127
-
 dynorphin A-(1-10)
-
 Rattus norvegicus
3.4.24.61 0.162
-
 dynorphin A-(1-9)
-
 Rattus norvegicus
3.4.24.61 0.209
-
 dynorphin B
-
 Rattus norvegicus
3.4.24.61 0.227
-
 [Arg0,Ala17]-somatostatin-28-(1-19)-NH2
-
 Rattus norvegicus
3.4.24.61 0.428
-
 [Ala17,Tyr20]-Somatostatin-28-(10-20)-NH2 or peptide I Rattus norvegicus
3.4.24.61 0.974
-
 alpha-neoendorphin
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.61 90000
-
 gel filtration Rattus norvegicus
3.4.24.61 90000
-
 rat, brain Rattus norvegicus
3.4.24.61 110000
-
 PAGE, 2 catalytically active forms: MW 110000 and MW 140000 Rattus norvegicus
3.4.24.61 110000
-
 rat, testis Rattus norvegicus
3.4.24.61 133000
-
 rat, testis, deduced from nucleotide sequence Rattus norvegicus
3.4.24.61 140000
-
 PAGE, 2 catalytically active forms: MW 110000 and MW 140000 Rattus norvegicus
3.4.24.61 140000
-
 rat, testis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.61 Rattus norvegicus
-
 male Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.61
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.61 brain
-
 Rattus norvegicus
-
3.4.24.61 hypophysis
-
 Rattus norvegicus
-
3.4.24.61 hypothalamus
-
 Rattus norvegicus
-
3.4.24.61 additional information tissue distribution Rattus norvegicus
-
3.4.24.61 neocortex
-
 Rattus norvegicus
-
3.4.24.61 testis predominantly Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.61 alpha-Neoendorphin + H2O i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Leu, cleavage at Arg-Lys processing site Rattus norvegicus Tyr-Gly-Gly-Phe-Leu-Arg + Lys-Tyr-Pro-Leu
-
 ?
3.4.24.61 ANF-(1-28) + H2O i.e. atrial natriuretic factor, cleavage site: Arg+Arg Rattus norvegicus ?
-
 ?
3.4.24.61 Bovine adrenal medulla dodecapeptide + H2O i.e. Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu Rattus norvegicus Tyr-Gly-Gly-Phe-Met-Arg + Arg-Val-Gly-Arg-Pro-Glu
-
 ?
3.4.24.61 dynorphin A + H2O i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln-NH2 Rattus norvegicus Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln-NH2
-
 ?
3.4.24.61 dynorphin A-(1-10) + H2O i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro, cleavage site: Arg-Arg Rattus norvegicus Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg-Pro
-
 ?
3.4.24.61 dynorphin A-(1-13) + H2O i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys, cleavage site: Arg-Arg Rattus norvegicus Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg-Pro-Lys-Leu-Lys
-
 ?
3.4.24.61 dynorphin A-(1-9) + H2O i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg, cleavage site: Arg-Arg Rattus norvegicus Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg
-
 ?
3.4.24.61 dynorphin B + H2O i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr Rattus norvegicus Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Gln-Phe-Lys-Val-Val-Thr
-
 ?
3.4.24.61 additional information [Tyr20]-somatostatin-28-(13-20)-NH2, dynorphin A-(1-7), [Ala17]-somatostatin-(6-19)-NH2, prosomatostatin-(56-68)-NH2, the Lys-Arg-doublet in preproenkephalin-(128-140) or pro-oxytocin/neurophysin-(1-20) Rattus norvegicus ?
-
 ?
3.4.24.61 additional information cleavage occurs selectively at amino-terminal side of an Arg-residue in dibasic stretches Rattus norvegicus ?
-
 ?
3.4.24.61 Preproneurotensin-(154-170) + H2O cleavage site: prohormonal maturation site, Lys-Arg Rattus norvegicus ?
-
 ?
3.4.24.61 [Ala17,Tyr20]-Somatostatin-28-(10-20)-NH2 + H2O i.e. model peptide I Rattus norvegicus Pro-Arg-Glu + Arg-Lys-Ala-Gly-Ala-Lys-Asn-Tyr-NH2
-
 ?
3.4.24.61 [Ala17,Tyr20]-Somatostatin-28-(10-20)-NH2 + H2O i.e. Pro-Arg-Glu-Arg-Lys-Ala-Gly-Ala-Lys-Asn-Tyr-NH2 Rattus norvegicus Pro-Arg-Glu + Arg-Lys-Ala-Gly-Ala-Lys-Asn-Tyr-NH2
-
 ?
3.4.24.61 [Arg0,Ala17]-somatostatin-28-(1-19)-NH2 + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.61 monomer 1 * 110000, testis, SDS-PAGE, 1 * 140000, brain, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.61 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.61 8.5
-
 peptide I as substrate Rattus norvegicus