BRENDA - Enzyme Database show

Two types of microsomal prostaglandin E synthase: glutathione-dependent and -independent prostaglandin E synthases

Watanabe, K.; Kurihara, K.; Tokunaga, Y.; Hayaishi, O.; Biochem. Biophys. Res. Commun. 235, 148-152 (1997)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
GSH
two different types of prostaglandin E synthase: 1. GSH-dependent enzyme, 2. GSH-independent enzyme. The genitally organs, especially the deferent duct have mainly GSH-dependent prostaglandin E synthase activity. Heart, spleen and uterus have only the GSH-independent activity. In absence of reduced glutathione the enzyme from deferent duct shows only 3% of the activity in presence of reduced glutathione
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.0257
-
prostaglandin H2
GSH-dependent enzyme from deferent duct
Rattus norvegicus
5.3.99.3
0.0826
-
prostaglandin H2
GSH-independent enzyme from heart
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Rattus norvegicus
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.3
adrenal gland
-
Rattus norvegicus
-
5.3.99.3
brain
-
Rattus norvegicus
-
5.3.99.3
caput epididymis
-
Rattus norvegicus
-
5.3.99.3
cauda epididymis
-
Rattus norvegicus
-
5.3.99.3
cecum
-
Rattus norvegicus
-
5.3.99.3
coagulating gland
-
Rattus norvegicus
-
5.3.99.3
heart
-
Rattus norvegicus
-
5.3.99.3
kidney
highest activity of nongenital organs
Rattus norvegicus
-
5.3.99.3
large intestine
-
Rattus norvegicus
-
5.3.99.3
lung
-
Rattus norvegicus
-
5.3.99.3
ovary
-
Rattus norvegicus
-
5.3.99.3
small intestine
-
Rattus norvegicus
-
5.3.99.3
spleen
-
Rattus norvegicus
-
5.3.99.3
stomach
-
Rattus norvegicus
-
5.3.99.3
testis
-
Rattus norvegicus
-
5.3.99.3
thymus
-
Rattus norvegicus
-
5.3.99.3
uterus
-
Rattus norvegicus
-
5.3.99.3
vas deferens
and other genital accessory organs
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
3134
Rattus norvegicus
Prostaglandin E2
-
3134
Rattus norvegicus
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.3
6
7
GSH-dependent enzyme from deferent duct
Rattus norvegicus
5.3.99.3
6
8
GSH-independent enzyme from heart
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.3.99.3
5
10
pH 5: about 75% of maximal activity, pH 10: about 70% of maximal activity, GSH-dependent enzyme from deferent duct
Rattus norvegicus
5.3.99.3
5
12
pH 5: about 60% of maximal activity, pH 9: about 75% of maximal activity, pH 12: about 90% of maximal activity, GSH-independent enzyme from heart
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
GSH
two different types of prostaglandin E synthase: 1. GSH-dependent enzyme, 2. GSH-independent enzyme. The genitally organs, especially the deferent duct have mainly GSH-dependent prostaglandin E synthase activity. Heart, spleen and uterus have only the GSH-independent activity. In absence of reduced glutathione the enzyme from deferent duct shows only 3% of the activity in presence of reduced glutathione
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.0257
-
prostaglandin H2
GSH-dependent enzyme from deferent duct
Rattus norvegicus
5.3.99.3
0.0826
-
prostaglandin H2
GSH-independent enzyme from heart
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Rattus norvegicus
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.3
adrenal gland
-
Rattus norvegicus
-
5.3.99.3
brain
-
Rattus norvegicus
-
5.3.99.3
caput epididymis
-
Rattus norvegicus
-
5.3.99.3
cauda epididymis
-
Rattus norvegicus
-
5.3.99.3
cecum
-
Rattus norvegicus
-
5.3.99.3
coagulating gland
-
Rattus norvegicus
-
5.3.99.3
heart
-
Rattus norvegicus
-
5.3.99.3
kidney
highest activity of nongenital organs
Rattus norvegicus
-
5.3.99.3
large intestine
-
Rattus norvegicus
-
5.3.99.3
lung
-
Rattus norvegicus
-
5.3.99.3
ovary
-
Rattus norvegicus
-
5.3.99.3
small intestine
-
Rattus norvegicus
-
5.3.99.3
spleen
-
Rattus norvegicus
-
5.3.99.3
stomach
-
Rattus norvegicus
-
5.3.99.3
testis
-
Rattus norvegicus
-
5.3.99.3
thymus
-
Rattus norvegicus
-
5.3.99.3
uterus
-
Rattus norvegicus
-
5.3.99.3
vas deferens
and other genital accessory organs
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
3134
Rattus norvegicus
Prostaglandin E2
-
3134
Rattus norvegicus
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.3
6
7
GSH-dependent enzyme from deferent duct
Rattus norvegicus
5.3.99.3
6
8
GSH-independent enzyme from heart
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.3.99.3
5
10
pH 5: about 75% of maximal activity, pH 10: about 70% of maximal activity, GSH-dependent enzyme from deferent duct
Rattus norvegicus
5.3.99.3
5
12
pH 5: about 60% of maximal activity, pH 9: about 75% of maximal activity, pH 12: about 90% of maximal activity, GSH-independent enzyme from heart
Rattus norvegicus