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Literature summary extracted from

  • Werlen, R.C.; Offord, R.E.; Rose, K.
    Preparation and characterization of novel substrates of insulin proteinase (EC 3.4.99.45) (1994), Biochem. J., 302, 907-911.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.56 1,10-phenanthroline
-
Rattus norvegicus
3.4.24.56 1,10-phenanthroline
-
Sus scrofa
3.4.24.56 bacitracin
-
Sus scrofa
3.4.24.56 Co2+
-
Rattus norvegicus
3.4.24.56 Co2+
-
Sus scrofa
3.4.24.56 Cu2+
-
Rattus norvegicus
3.4.24.56 Cu2+
-
Sus scrofa
3.4.24.56 EDTA
-
Rattus norvegicus
3.4.24.56 EDTA
-
Sus scrofa
3.4.24.56 Fragment of cytochrome c
-
Sus scrofa
3.4.24.56 Mn2+
-
Rattus norvegicus
3.4.24.56 Mn2+
-
Sus scrofa
3.4.24.56 additional information not: bestatine Rattus norvegicus
3.4.24.56 additional information not: benzamidine; not: bestatine; not: phenylmethanesulfonyl fluoride Sus scrofa
3.4.24.56 NEM
-
Rattus norvegicus
3.4.24.56 NEM
-
Sus scrofa
3.4.24.56 Ni2+
-
Rattus norvegicus
3.4.24.56 Ni2+
-
Sus scrofa
3.4.24.56 Tryptic fragment of bovine serum albumin
-
Sus scrofa
3.4.24.56 Zn2+
-
Rattus norvegicus
3.4.24.56 Zn2+
-
Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.56 Ca2+
-
Rattus norvegicus
3.4.24.56 Ca2+ activates Sus scrofa
3.4.24.56 Mg2+ activates Rattus norvegicus
3.4.24.56 Mg2+ activates Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.56 110000
-
x * 110000, pig, SDS-PAGE Rattus norvegicus
3.4.24.56 110000
-
x * 110000, pig, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Rattus norvegicus
-
-
-
3.4.24.56 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.56
-
Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.56 skeletal muscle
-
Rattus norvegicus
-
3.4.24.56 skeletal muscle
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.56 additional information
-
-
Rattus norvegicus
3.4.24.56 additional information
-
-
Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 Fragment of cytochrome c + H2O Ile81-Glu108 Sus scrofa Hydrolyzed fragment of cytochrome c cleavage at Tyr97-Leu98 bond ?
3.4.24.56 Insulin + H2O
-
Rattus norvegicus Hydrolyzed insulin
-
?
3.4.24.56 Insulin + H2O
-
Sus scrofa Hydrolyzed insulin
-
?
3.4.24.56 additional information
-
Rattus norvegicus ?
-
?
3.4.24.56 additional information preference for small substrates 2000-6000 MW Sus scrofa ?
-
?
3.4.24.56 Tryptic fragment of bovine serum albumin + H2O Leu503-Lys518 Sus scrofa Hydrolyzed tryptic fragment of bovine serum albumin cleaved at Phe506-His507 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.56 ? x * 110000, pig, SDS-PAGE Rattus norvegicus
3.4.24.56 ? x * 110000, pig, SDS-PAGE Sus scrofa