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Literature summary extracted from

  • Fricke, B.; Betz, R.; Friebe, S.
    A periplasmic insulin-cleaving proteinase (ICP) from Acinetobacter calcoaceticus sharing properties with protease III from Escherichia coli and IDE from eucaryotes (1995), J. Basic Microbiol., 35, 21-31.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.55 1,10-phenanthroline Co2+, and to a lesser extent but at lower metal ion concentration Zn2+ restores activity Acinetobacter calcoaceticus
3.4.24.55 1,10-phenanthroline Co2+, and to a lesser extent but at lower metal ion concentration Zn2+ restores activity Escherichia coli
3.4.24.55 antipain
-
 Acinetobacter calcoaceticus
3.4.24.55 chymostatin
-
 Acinetobacter calcoaceticus
3.4.24.55 Co2+
-
 Acinetobacter calcoaceticus
3.4.24.55 Co2+
-
 Escherichia coli
3.4.24.55 DTT
-
 Acinetobacter calcoaceticus
3.4.24.55 DTT
-
 Escherichia coli
3.4.24.55 EDTA Co2+, and to a lesser extent but at lower metal ion concentration Zn2+ restores activity Acinetobacter calcoaceticus
3.4.24.55 EDTA Co2+, and to a lesser extent but at lower metal ion concentration Zn2+ restores activity Escherichia coli
3.4.24.55 PCMB
-
 Acinetobacter calcoaceticus
3.4.24.55 phosphoramidon
-
 Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.55 0.000105
-
 Insulin
-
 Acinetobacter calcoaceticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.55 periplasm
-
 Escherichia coli
-
-
3.4.24.55 periplasm
-
 Acinetobacter calcoaceticus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.55 Co2+ reactivation after inhibition with chelating agents Escherichia coli
3.4.24.55 Co2+ reactivation after inhibition with chelating agents Acinetobacter calcoaceticus
3.4.24.55 Zn2+ most effective metal ion in reactivation after inhibition by chelating agent Escherichia coli
3.4.24.55 Zn2+ most effective metal ion in reactivation after inhibition by chelating agent Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.55 107000
-
 1 * 107000, Acinetobacter calcoaceticus, SDS-PAGE Escherichia coli
3.4.24.55 107000
-
 1 * 107000, Acinetobacter calcoaceticus, SDS-PAGE Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.55 Acinetobacter calcoaceticus
-
-
-
3.4.24.55 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.55 similar enzyme Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.55 Glucagon + H2O
-
 Escherichia coli ?
-
 ?
3.4.24.55 Glucagon + H2O
-
 Acinetobacter calcoaceticus ?
-
 ?
3.4.24.55 Insulin + H2O
-
 Escherichia coli Hydrolyzed insulin
-
 ?
3.4.24.55 Insulin + H2O
-
 Acinetobacter calcoaceticus Hydrolyzed insulin
-
 ?
3.4.24.55 Insulin B-chain + H2O
-
 Escherichia coli ?
-
 ?
3.4.24.55 Insulin B-chain + H2O cleavage sites are Asn3-Gln4, His10-Leu11, Ala14-Leu15, Leu17-Val18, Gly23-Phe24, Phe24-Phe25, Phe25-Tyr26, the enzyme shares one of the only two cleavage sites with pitrilysin and 4 sites with insulin-destroying insulinase (EC 3.4.24.56) Acinetobacter calcoaceticus ?
-
 ?
3.4.24.55 additional information
-
 Escherichia coli ?
-
 ?
3.4.24.55 additional information prefers the cleavage of small polypeptides to the cleavage of proteins, inactive against synthetic amino acid derivatives of subtilisin, thermolysin, trypsin and chymotrypsin Acinetobacter calcoaceticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.55 monomer 1 * 107000, Acinetobacter calcoaceticus, SDS-PAGE Escherichia coli
3.4.24.55 monomer 1 * 107000, Acinetobacter calcoaceticus, SDS-PAGE Acinetobacter calcoaceticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.55 45
-
-
 Escherichia coli
3.4.24.55 45
-
-
 Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.55 6.8 7.2 insulin Acinetobacter calcoaceticus