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Literature summary extracted from

  • Anastasi, A.; Knight, C.G.; Barrett, A.J.
    Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay (1993), Biochem. J., 290, 601-607.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.55 1,10-phenanthroline
-
 Escherichia coli
3.4.24.55 2,6-pyridinedicarboxylic acid
-
 Escherichia coli
3.4.24.55 bacitracin especially in the presence of zinc Escherichia coli
3.4.24.55 Ca2+ 1 mM, weak, reactivates after inhibition with chelating agents Escherichia coli
3.4.24.55 Co2+ 1 mM Escherichia coli
3.4.24.55 DTT
-
 Escherichia coli
3.4.24.55 dynorphin A(1-13)
-
 Escherichia coli
3.4.24.55 EDTA
-
 Escherichia coli
3.4.24.55 Mn2+ 1 mM, weak, reactivates after inhibition with chelating agents Escherichia coli
3.4.24.55 additional information alpha-macroglobulin Escherichia coli
3.4.24.55 Tetraethylenepentamine
-
 Escherichia coli
3.4.24.55 Zincov
-
 Escherichia coli
3.4.24.55 Zn2+ 1 mM inhibits, but reactivates after inhibition with chelating agents Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.55 0.0077
-
 (7-methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-Lys(2,4-dinitrophenyl)-Leu-Asp-D-Lys
-
 Escherichia coli
3.4.24.55 0.0127
-
 glucagon
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.55 periplasm
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.55 Co2+ reactivation after inhibition with chelating agents Escherichia coli
3.4.24.55 Mn2+ reactivation after inhibition with chelating agents Escherichia coli
3.4.24.55 Zn2+ most effective metal ion in reactivation after inhibition by chelating agent Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.55 116000
-
 1 * 116000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.55 Escherichia coli
-
 overexpressing strain
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.55 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.55 (7-Methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-(2,4-dinitrophenyl)Lys-Leu-Asp-D-Lys + H2O
-
 Escherichia coli ?
-
 ?
3.4.24.55 (7-methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-Lys(2,4-dinitrophenyl)-Leu-Asp-D-Lys + H2O
-
 Escherichia coli ?
-
 ?
3.4.24.55 Glucagon + H2O
-
 Escherichia coli ?
-
 ?
3.4.24.55 Insulin B-chain + H2O small peptides down to 10 residues in length are cleaved more slowly, intact insulin is cleaved very slowly Escherichia coli ?
-
 ?
3.4.24.55 additional information the activity of the enzyme is confined to substrates smaller than proteins Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.55 monomer 1 * 116000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.55 0.05
-
 glucagon
-
 Escherichia coli