BRENDA - Enzyme Database show

Prostaglandin endoperoxide E isomerase from bovine vesicular gland microsomes, a glutathione-requiring enzyme

Ogino, N.; Miyamoto, T.; Yamamoto, S.; Hayaishi, O.; J. Biol. Chem. 252, 890-895 (1977)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
GSH
dependent on
Bos taurus
General Stability
EC Number
General Stability
Organism
5.3.99.3
thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or Cys markedly protect from thermal inactivation
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.3
Bis(3-carboxy-4-nitrophenyl)disulfide
-
Bos taurus
5.3.99.3
HgCl2
-
Bos taurus
5.3.99.3
iodoacetamide
-
Bos taurus
5.3.99.3
iodoacetic acid
-
Bos taurus
5.3.99.3
Iodosobenzoic acid
-
Bos taurus
5.3.99.3
NEM
-
Bos taurus
5.3.99.3
PCMB
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.01
-
Prostaglandin H1
-
Bos taurus
5.3.99.3
0.05
-
Prostaglandin G1
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Bos taurus
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Bos taurus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.3
1.6
-
prostaglandin H1
Bos taurus
5.3.99.3
1.8
-
prostaglandin H2
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
5.3.99.3
-20°C, 1 mM glutathione, pH 7.0, completely inactivated after a few weeks
Bos taurus
5.3.99.3
-70°C, 1 mM glutathione, pH 7.0, stable for at least several months
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin G1
at 50% of the activity relative to prostaglandin H
3129
Bos taurus
15-Hydroperoxy-prostaglandin E1
major product
3129
Bos taurus
-
5.3.99.3
prostaglandin G2
at 50% of the activity relative to prostaglandin H
3129
Bos taurus
15-hydroperoxy-prostaglandin E2
major product
3129
Bos taurus
-
5.3.99.3
Prostaglandin H1
ir
3129
Bos taurus
Prostaglandin E1
-
3129
Bos taurus
-
5.3.99.3
Prostaglandin H1
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-13-enoic acid
3129
Bos taurus
Prostaglandin E1
-
3129
Bos taurus
-
5.3.99.3
Prostaglandin H2
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
3129
Bos taurus
Prostaglandin E2
-
3129
Bos taurus
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5.3.99.3
additional information
-
thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or cysteine markedly protect from thermal inactivation
Bos taurus
5.3.99.3
25
-
pH 8.0, 0.1 M potassium phosphate buffer, half-life: 30 min
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.3
7
8
-
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.3.99.3
5.5
9.5
almost inactive below pH 5.5 and above pH 9.5
Bos taurus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
5.3.99.3
7
8
pH-range of highest stability, 35°C, 7.5 min, either in presence or absence of glutathione
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
GSH
dependent on
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
5.3.99.3
thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or Cys markedly protect from thermal inactivation
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.3
Bis(3-carboxy-4-nitrophenyl)disulfide
-
Bos taurus
5.3.99.3
HgCl2
-
Bos taurus
5.3.99.3
iodoacetamide
-
Bos taurus
5.3.99.3
iodoacetic acid
-
Bos taurus
5.3.99.3
Iodosobenzoic acid
-
Bos taurus
5.3.99.3
NEM
-
Bos taurus
5.3.99.3
PCMB
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.01
-
Prostaglandin H1
-
Bos taurus
5.3.99.3
0.05
-
Prostaglandin G1
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Bos taurus
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.3
1.6
-
prostaglandin H1
Bos taurus
5.3.99.3
1.8
-
prostaglandin H2
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
5.3.99.3
-20°C, 1 mM glutathione, pH 7.0, completely inactivated after a few weeks
Bos taurus
5.3.99.3
-70°C, 1 mM glutathione, pH 7.0, stable for at least several months
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin G1
at 50% of the activity relative to prostaglandin H
3129
Bos taurus
15-Hydroperoxy-prostaglandin E1
major product
3129
Bos taurus
-
5.3.99.3
prostaglandin G2
at 50% of the activity relative to prostaglandin H
3129
Bos taurus
15-hydroperoxy-prostaglandin E2
major product
3129
Bos taurus
-
5.3.99.3
Prostaglandin H1
ir
3129
Bos taurus
Prostaglandin E1
-
3129
Bos taurus
-
5.3.99.3
Prostaglandin H1
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-13-enoic acid
3129
Bos taurus
Prostaglandin E1
-
3129
Bos taurus
-
5.3.99.3
Prostaglandin H2
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
3129
Bos taurus
Prostaglandin E2
-
3129
Bos taurus
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5.3.99.3
additional information
-
thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or cysteine markedly protect from thermal inactivation
Bos taurus
5.3.99.3
25
-
pH 8.0, 0.1 M potassium phosphate buffer, half-life: 30 min
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.3
7
8
-
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.3.99.3
5.5
9.5
almost inactive below pH 5.5 and above pH 9.5
Bos taurus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
5.3.99.3
7
8
pH-range of highest stability, 35°C, 7.5 min, either in presence or absence of glutathione
Bos taurus