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Literature summary extracted from

  • Ogino, N.; Miyamoto, T.; Yamamoto, S.; Hayaishi, O.
    Prostaglandin endoperoxide E isomerase from bovine vesicular gland microsomes, a glutathione-requiring enzyme (1977), J. Biol. Chem., 252, 890-895.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.99.3 GSH dependent on Bos taurus

General Stability

EC Number General Stability Organism
5.3.99.3 thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or Cys markedly protect from thermal inactivation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.99.3 Bis(3-carboxy-4-nitrophenyl)disulfide
-
 Bos taurus
5.3.99.3 HgCl2
-
 Bos taurus
5.3.99.3 iodoacetamide
-
 Bos taurus
5.3.99.3 iodoacetic acid
-
 Bos taurus
5.3.99.3 Iodosobenzoic acid
-
 Bos taurus
5.3.99.3 NEM
-
 Bos taurus
5.3.99.3 PCMB
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.99.3 0.01
-
 Prostaglandin H1
-
 Bos taurus
5.3.99.3 0.05
-
 Prostaglandin G1
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.3 microsome
-
 Bos taurus
-
-

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.3 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.99.3 1.6
-
 prostaglandin H1 Bos taurus
5.3.99.3 1.8
-
 prostaglandin H2 Bos taurus

Storage Stability

EC Number Storage Stability Organism
5.3.99.3 -20°C, 1 mM glutathione, pH 7.0, completely inactivated after a few weeks Bos taurus
5.3.99.3 -70°C, 1 mM glutathione, pH 7.0, stable for at least several months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.3 Prostaglandin G1 at 50% of the activity relative to prostaglandin H Bos taurus 15-Hydroperoxy-prostaglandin E1 major product ?
5.3.99.3 prostaglandin G2 at 50% of the activity relative to prostaglandin H Bos taurus 15-hydroperoxy-prostaglandin E2 major product ?
5.3.99.3 Prostaglandin H1 ir Bos taurus Prostaglandin E1
-
 ?
5.3.99.3 Prostaglandin H1 i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-13-enoic acid Bos taurus Prostaglandin E1
-
 ?
5.3.99.3 Prostaglandin H2 i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid Bos taurus Prostaglandin E2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.99.3 additional information
-
 thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or cysteine markedly protect from thermal inactivation Bos taurus
5.3.99.3 25
-
 pH 8.0, 0.1 M potassium phosphate buffer, half-life: 30 min Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.99.3 7 8
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.99.3 5.5 9.5 almost inactive below pH 5.5 and above pH 9.5 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.99.3 7 8 pH-range of highest stability, 35°C, 7.5 min, either in presence or absence of glutathione Bos taurus