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Literature summary extracted from

  • Civello, D.J.; Duong, H.L.; Geren, C.R.
    Isolation and characterization of a hemorrhagic proteinase from timber rattlesnake venom (1983), Biochemistry, 22, 749-755.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.24.47 Extensive dialysis against distilled water inactivates, incubation with Zn2+, Ca2+ or Mg2+ fails to reconstitute activity Crotalus horridus horridus
3.4.24.47 Stable to dialysis against 0.2 M Tris buffer, pH 7.5 Crotalus horridus horridus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.47 1,10-phenanthroline
-
 Crotalus horridus horridus
3.4.24.47 dithiothreitol
-
 Crotalus horridus horridus
3.4.24.47 EDTA
-
 Crotalus horridus horridus
3.4.24.47 additional information no inhibition by alpha1-antitrypsin, N-ethylmaleimide, iodoacetamide, phenylmethylsulfonylfluoride, egg white or soybean trypsin inhibitor Crotalus horridus horridus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.47 Ca2+ zinc and calcium containing metalloproteinase, no activation by added Ca2+ Crotalus horridus horridus
3.4.24.47 Mg2+ 0.14 mol/mol enzyme Crotalus horridus horridus
3.4.24.47 Zn2+ 1 mol zinc/mol enzyme Crotalus horridus horridus
3.4.24.47 Zn2+ zinc and calcium containing metalloproteinase Crotalus horridus horridus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.47 additional information
-
 amino acid composition compared to other rattlesnake venom proteins Crotalus horridus horridus
3.4.24.47 52000
-
 Crotalus horridus horridus, gel filtration Crotalus horridus horridus
3.4.24.47 56000
-
 1 * 56000, Crotalus horridus horridus, SDS-PAGE Crotalus horridus horridus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.47 additional information Crotalus horridus horridus hemorrhage inducing toxin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.47 Crotalus horridus horridus
-
 Timber rattlesnake
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.47 additional information no glycoprotein Crotalus horridus horridus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.47
-
 Crotalus horridus horridus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.47 venom
-
 Crotalus horridus horridus
-

Storage Stability

EC Number Storage Stability Organism
3.4.24.47 -20°C, in either lyophilized or frozen state, at least 3 years Crotalus horridus horridus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.47 casein + H2O denatured casein, poor substrate Crotalus horridus horridus ?
-
 ?
3.4.24.47 Hemoglobin + H2O poor substrate Crotalus horridus horridus ?
-
 ?
3.4.24.47 hide powder azure + H2O
-
 Crotalus horridus horridus ?
-
 ?
3.4.24.47 additional information
-
 Crotalus horridus horridus ?
-
 ?
3.4.24.47 additional information hemorrhage inducing toxin Crotalus horridus horridus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.47 monomer 1 * 56000, Crotalus horridus horridus, SDS-PAGE Crotalus horridus horridus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.47 37
-
 assay at Crotalus horridus horridus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.47 additional information
-
 pI: 5.1 Crotalus horridus horridus
3.4.24.47 7.5
-
 hide powder azure as substrate, 0.2 M Tris buffer Crotalus horridus horridus