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Literature summary extracted from

  • Nikai, T.; Mori, N.; Kishida, M.; Sugihara, H.; Tu, A.T.
    Isolation and biochemical characterization of hemorrhagic toxin f from the venom of Crotalus atrox (western diamondback rattlesnake) (1984), Arch. Biochem. Biophys., 231, 309-319.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.45 1,10-phenanthroline casein as substrate Crotalus atrox
3.4.24.45 EDTA casein as substrate Crotalus atrox

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.45 Zn2+ zinc metalloproteinase, 1 mol zinc/mol enzyme Crotalus atrox
3.4.24.45 Zn2+ essential for hemorrhagic and proteolytic activity Crotalus atrox

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.45 additional information
-
 amino acid composition, immunologically not related to hemorrhagic toxin a or c Crotalus atrox
3.4.24.45 64000
-
 1 * 64000, Crotalus atrox, SDS-PAGE Crotalus atrox

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.45 Basement membranes surrounding capillaries + H2O Crotalus atrox produces not only local hemorrhage in skin and muscle, but also systemic hemorrhage in internal organs such as intestine, kidney, lung, heart and liver ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.45 Crotalus atrox
-
 Western diamondback rattlesnake
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.45 from lyophilized crude venom Crotalus atrox

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.45 venom
-
 Crotalus atrox
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.45 Basement membranes surrounding capillaries + H2O produces not only local hemorrhage in skin and muscle, but also systemic hemorrhage in internal organs such as intestine, kidney, lung, heart and liver Crotalus atrox ?
-
 ?
3.4.24.45 casein + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.45 Dimethylcasein + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.45 Fibrinogen + H2O no coagulation Crotalus atrox Hydrolyzed fibrinogen non-clottable products ?
3.4.24.45 Fibrinogen + H2O cleaves gamma-chain, Aalpha and Bbeta-chains remain intact Crotalus atrox Hydrolyzed fibrinogen non-clottable products ?
3.4.24.45 additional information no hydrolysis of tosyl-L-Arg methyl ester or N-benzoyl-L-Arg ethyl ester Crotalus atrox ?
-
 ?
3.4.24.45 Oxidized insulin B-chain + H2O cleaves Val2-Asn3, Gln4-His5, Leu6-Cys7, H10-Leu11, Ala14-Leu15, Tyr16-Leu17 bonds Crotalus atrox Hydrolyzed oxidized insulin B-chain several peptides ?

Subunits

EC Number Subunits Comment Organism
3.4.24.45 monomer in the presence of 2-mercaptoethanol Crotalus atrox
3.4.24.45 monomer 1 * 64000, Crotalus atrox, SDS-PAGE Crotalus atrox

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.45 37
-
 assay at Crotalus atrox

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.45 additional information
-
 pI: 7.7 Crotalus atrox