BRENDA - Enzyme Database show

Modulation of prostacyclin synthetase and unmasking of PGE2 isomerase in bovine coronary arterial microsomes

McNamara, D.B.; Hussey, J.L.; Kerstein, M.D.; Rosenson, R.S.; Hyman, A.L.; Kadowitz, P.J.; Biochem. Biophys. Res. Commun. 118, 33-39 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
dithiothreitol
activates
Bos taurus
5.3.99.3
dithiothreitol
activates
Canis lupus familiaris
5.3.99.3
dithiothreitol
activates
Homo sapiens
5.3.99.3
dithiothreitol
activates
Ovis aries
5.3.99.3
GSH
activates
Bos taurus
5.3.99.3
GSH
activates
Canis lupus familiaris
5.3.99.3
GSH
activates
Homo sapiens
5.3.99.3
GSH
activates
Ovis aries
5.3.99.4
dithiothreitol
increase in activity with increasing glutathione concentration from 0.1 mM up to 5 mM
Bos taurus
5.3.99.4
reduced glutathione
increase in activity with increasing glutathione concentration from 0.1 mM up to 5 mM
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.4
Tranylcypromine
10 mM, 90% inhibition in absence or presence of 2 mM reduced glutathione
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Ovis aries
-
-
5.3.99.3
microsome
-
Bos taurus
-
-
5.3.99.4
microsome
-
Ovis aries
-
-
5.3.99.4
microsome
-
Bos taurus
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Bos taurus
-
-
-
5.3.99.3
Canis lupus familiaris
-
-
-
5.3.99.3
Homo sapiens
-
-
-
5.3.99.3
Ovis aries
-
-
-
5.3.99.4
Bos taurus
-
-
-
5.3.99.4
Canis lupus familiaris
-
-
-
5.3.99.4
Homo sapiens
-
-
-
5.3.99.4
Ovis aries
-
ram
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
5.3.99.4
the prostacyclin synthase is unusually sensitive to the redox state or sulfhydryl oxidation of the enzyme
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.3
coronary artery
-
Bos taurus
-
5.3.99.4
coronary artery
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
3127
Bos taurus
Prostaglandin E2
-
3127
Bos taurus
-
5.3.99.3
Prostaglandin H2
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
3127
Ovis aries
Prostaglandin E2
-
3127
Ovis aries
-
5.3.99.4
Prostaglandin H2
-
3127
Homo sapiens
Prostaglandin I2
prostaglandin I2
3127
Homo sapiens
-
5.3.99.4
Prostaglandin H2
-
3127
Bos taurus
Prostaglandin I2
prostaglandin I2
3127
Bos taurus
-
5.3.99.4
Prostaglandin H2
-
3127
Ovis aries
Prostaglandin I2
prostaglandin I2
3127
Ovis aries
-
5.3.99.4
Prostaglandin H2
-
3127
Canis lupus familiaris
Prostaglandin I2
prostaglandin I2
3127
Canis lupus familiaris
-
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
dithiothreitol
activates
Bos taurus
5.3.99.3
dithiothreitol
activates
Canis lupus familiaris
5.3.99.3
dithiothreitol
activates
Homo sapiens
5.3.99.3
dithiothreitol
activates
Ovis aries
5.3.99.3
GSH
activates
Bos taurus
5.3.99.3
GSH
activates
Canis lupus familiaris
5.3.99.3
GSH
activates
Homo sapiens
5.3.99.3
GSH
activates
Ovis aries
5.3.99.4
dithiothreitol
increase in activity with increasing glutathione concentration from 0.1 mM up to 5 mM
Bos taurus
5.3.99.4
reduced glutathione
increase in activity with increasing glutathione concentration from 0.1 mM up to 5 mM
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.4
Tranylcypromine
10 mM, 90% inhibition in absence or presence of 2 mM reduced glutathione
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Ovis aries
-
-
5.3.99.3
microsome
-
Bos taurus
-
-
5.3.99.4
microsome
-
Ovis aries
-
-
5.3.99.4
microsome
-
Bos taurus
-
-
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
5.3.99.4
the prostacyclin synthase is unusually sensitive to the redox state or sulfhydryl oxidation of the enzyme
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.3
coronary artery
-
Bos taurus
-
5.3.99.4
coronary artery
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
3127
Bos taurus
Prostaglandin E2
-
3127
Bos taurus
-
5.3.99.3
Prostaglandin H2
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
3127
Ovis aries
Prostaglandin E2
-
3127
Ovis aries
-
5.3.99.4
Prostaglandin H2
-
3127
Homo sapiens
Prostaglandin I2
prostaglandin I2
3127
Homo sapiens
-
5.3.99.4
Prostaglandin H2
-
3127
Bos taurus
Prostaglandin I2
prostaglandin I2
3127
Bos taurus
-
5.3.99.4
Prostaglandin H2
-
3127
Ovis aries
Prostaglandin I2
prostaglandin I2
3127
Ovis aries
-
5.3.99.4
Prostaglandin H2
-
3127
Canis lupus familiaris
Prostaglandin I2
prostaglandin I2
3127
Canis lupus familiaris
-