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Literature summary extracted from

  • Bjarnason, J.B.; Fox, J.W.
    Characterization of two hemorrhagic zinc proteinases, toxin c and toxin d, from western diamondback rattlesnake (Crotalus atrox) venom (1987), Biochim. Biophys. Acta, 911, 356-363.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.1 EDTA
-
 Crotalus atrox
3.4.24.1 o-phenanthroline
-
 Crotalus atrox
3.4.24.42 1,10-phenanthroline
-
 Crotalus atrox
3.4.24.42 EDTA
-
 Crotalus atrox
3.4.24.42 additional information no inhibition by aprotinin, PMSF Crotalus atrox

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.1 Zn2+ zinc metalloproteinase Crotalus atrox
3.4.24.42 Zn2+ zinc metalloproteinase Crotalus atrox
3.4.24.42 Zn2+ 0.86 mol zinc/mol enzyme Crotalus atrox

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.1 24000
-
 x * 24000, Ht-d, Ht-c, SDS-PAGE Crotalus atrox
3.4.24.42 additional information
-
 amino acid composition Crotalus atrox
3.4.24.42 additional information
-
 isozymes Cc and Cd share identical antigenic structures Crotalus atrox

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.1 Proteins + H2O Crotalus atrox preferential cleavage: -Leu, -Ile, -Val, -Phe ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.1 Crotalus atrox
-
-
-
3.4.24.42 Crotalus atrox
-
 Western diamondback rattlesnake
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.42 additional information no glycoprotein Crotalus atrox

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.1 venom
-
 Crotalus atrox
-
3.4.24.42 venom
-
 Crotalus atrox
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.1 Proteins + H2O preferential cleavage: -Leu, -Ile, -Val, -Phe Crotalus atrox ?
-
 ?
3.4.24.42 hide powder azure + H2O
-
 Crotalus atrox ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.1 More x * 24000, Ht-d, Ht-c, SDS-PAGE Crotalus atrox

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.1 additional information
-
 additional information
-
 Crotalus atrox

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.1 8 9
-
 Crotalus atrox
3.4.24.42 additional information
-
 pI: 6.2 (isozyme Ht-c) Crotalus atrox
3.4.24.42 additional information
-
 pI: 6.1 (isozyme Ht-d) Crotalus atrox
3.4.24.42 8.3
-
 hide powder azure, isozyme Ht-c Crotalus atrox
3.4.24.42 9
-
 hide powder azure, isozyme Ht-d Crotalus atrox

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.1 7 10 pH 7 or pH 10: about 15% of activity maximum Crotalus atrox
3.4.24.42 7.3 10 about half-maximal activity at pH 7.3 and 10, isozyme Ht-d Crotalus atrox
3.4.24.42 7.5 9.8 about half-maximal activity at pH 7.5 and 9.8, isozyme Ht-c Crotalus atrox