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Literature summary extracted from
- Studies on the mechanism of hemorrhage production by five proteolytic hemorrhagic toxins from Crotalus atrox venom (1988), Biol. Chem. Hoppe-Seyler, 369, 121-129.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.41 | 1,10-phenanthroline | - |
Crotalus atrox |  |
| 3.4.24.41 | EDTA | - |
Crotalus atrox | |
| 3.4.24.41 | Hemorrhagic toxin inhibitor from Crotalus atrox | - |
Crotalus atrox | |
| 3.4.24.42 | 1,10-phenanthroline | - |
Crotalus atrox | |
| 3.4.24.42 | Amino acid hydroxamates | - |
Crotalus atrox | |
| 3.4.24.42 | EDTA | - |
Crotalus atrox | |
| 3.4.24.44 | 1,10-phenanthroline | - |
Crotalus atrox | |
| 3.4.24.44 | EDTA | - |
Crotalus atrox | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.41 | Zn2+ | zinc metalloproteinase, 1 mol zinc/mol enzyme | Crotalus atrox | |
| 3.4.24.42 | Zn2+ | zinc metalloproteinase | Crotalus atrox | |
| 3.4.24.42 | Zn2+ | 1 mol zinc/mol enzyme | Crotalus atrox | |
| 3.4.24.44 | Zn2+ | zinc metalloproteinase | Crotalus atrox | |
| 3.4.24.44 | Zn2+ | 1 mol zinc/mol enzyme | Crotalus atrox | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.41 | 24000 | - |
1 * 24000, Crotalus atrox, SDS-PAGE | Crotalus atrox |
| 3.4.24.44 | 25700 | - |
1 * 25700, Crotalus atrox, SDS-PAGE | Crotalus atrox |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.41 | Crotalus atrox | - |
Western diamondback rattlesnake | - |
| 3.4.24.42 | Crotalus atrox | - |
Western diamondback rattlesnake | - |
| 3.4.24.44 | Crotalus atrox | - |
Western diamondback rattlesnake | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.41 | venom | - |
Crotalus atrox | - |
| 3.4.24.42 | venom | - |
Crotalus atrox | - |
| 3.4.24.44 | venom | - |
Crotalus atrox | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.41 | Basement membrane preparation + H2O | poor substrate: component band a | Crotalus atrox | Soluble peptides | - |
? | |
| 3.4.24.41 | Laminin + H2O | basement membrane component, poor substrate | Crotalus atrox | ? | - |
? | |
| 3.4.24.41 | additional information | no hydrolysis of fibrin | Crotalus atrox | ? | - |
? | |
| 3.4.24.41 | additional information | no hydrolysis of fibronectin | Crotalus atrox | ? | - |
? | |
| 3.4.24.41 | Nidogen + H2O | basement membrane component | Crotalus atrox | ? | - |
? | |
| 3.4.24.41 | Oxidized insulin B-chain + H2O | cleavage sites: His5-Leu6, His10-Leu11, Ala14-Leu15, Tyr16-Leu17 (atrolysin A and C cleave the same bonds) and Gly23-Phe24 (atrolysin C, EC 3.4.24.42 cleaves the same bond) | Crotalus atrox | ? | - |
? | |
| 3.4.24.42 | Basement membrane preparation + H2O | poor substrate: component band a | Crotalus atrox | Soluble peptides | - |
? | |
| 3.4.24.42 | Laminin + H2O | basement membrane component, poor substrate | Crotalus atrox | ? | - |
? | |
| 3.4.24.42 | Oxidized insulin B-chain + H2O | cleavage at Gly23-Phe24 | Crotalus atrox | ? | - |
? | |
| 3.4.24.42 | Oxidized insulin B-chain + H2O | cleaves the same bonds as atrolysin B and A (the latter except Gly23-Phe24) | Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | Basement membrane preparation + H2O | component band a, most active on this substrate | Crotalus atrox | Soluble peptides | - |
? | |
| 3.4.24.44 | Fibrinogen + H2O | - |
Crotalus atrox | Hydrolyzed fibrinogen | non-clotting products | ? | |
| 3.4.24.44 | Laminin + H2O | basement membrane component, most active on this substrate | Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | additional information | no hydrolysis of fibrin | Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | Nidogen + H2O | - |
Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | Oxidized insulin B-chain + H2O | - |
Crotalus atrox | Hydrolyzed oxidized insulin B-chain | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.41 | monomer | 1 * 24000, Crotalus atrox, SDS-PAGE | Crotalus atrox |
| 3.4.24.44 | monomer | 1 * 25700, Crotalus atrox, SDS-PAGE | Crotalus atrox |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.41 | 37 | - |
assay at | Crotalus atrox |
| 3.4.24.42 | 37 | - |
assay at | Crotalus atrox |
| 3.4.24.44 | 37 | - |
assay at | Crotalus atrox |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.41 | 0.0267 | - |
oxidized insulin B-chain | Gly23-Phe24 bond | Crotalus atrox | |
| 3.4.24.41 | 0.0317 | - |
oxidized insulin B-chain | His5-Leu6 bond | Crotalus atrox | |
| 3.4.24.41 | 0.132 | - |
oxidized insulin B-chain | His10-Leu11 bond | Crotalus atrox | |
| 3.4.24.41 | 0.803 | - |
oxidized insulin B-chain | Ala14-Leu15 bond | Crotalus atrox | |
| 3.4.24.41 | 1.02 | - |
oxidized insulin B-chain | Tyr16-Leu17 bond | Crotalus atrox |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.41 | additional information | - |
basic pI | Crotalus atrox |
| 3.4.24.42 | additional information | - |
pI: 6 (isozyme Cc), pI: 6.1 (isozyme Cd) | Crotalus atrox |
| 3.4.24.44 | additional information | - |
pI: 5.6 | Crotalus atrox |
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