BRENDA - Enzyme Database show

Immunochemical and kinetic evidence for two different prostaglandin H-prostaglandin E isomerases in sheep vesicular gland microsomes

Tanaka, Y.; Ward, S.L.; Smith, W.L.; J. Biol. Chem. 262, 1374-1381 (1987)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
GSH
2 mM required for maximal activity of IgG1(hei-7)-reactive protein; concentrations below 0.5 mM are required for maximal activity of IgG1(hei-26)-reactive protein; dependent on
Ovis aries
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.04
-
prostaglandin H2
IgG1(hei-7)-reactive protein
Ovis aries
5.3.99.3
0.15
-
prostaglandin H2
IgG1(hei-26)-reactive protein
Ovis aries
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
at least 2 different proteins with PGE-PGH isomerase activity
Ovis aries
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
17500
-
x * 17500, IgG1(hei-7)-reactive protein, SDS-PAGE
Ovis aries
5.3.99.3
180000
-
x * 180000, IgG1(hei-26)-reactive protein, SDS-PAGE
Ovis aries
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Ovis aries
-
at least 2 different proteins with PGE-PGH isomerase activity: IgG1(hei-7)-reactive protein and IgG1(hei-26)-reactive protein
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.3
-
Ovis aries
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.3
epithelium
at least 2 different proteins with PGE-PGH isomerase activity; epithelium cells
Ovis aries
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
3126
Ovis aries
Prostaglandin E2
-
3126
Ovis aries
-
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.3
?
x * 17500, IgG1(hei-7)-reactive protein, SDS-PAGE; x * 180000, IgG1(hei-26)-reactive protein, SDS-PAGE
Ovis aries
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.3
6.2
-
IgG1(hei-26)-reactive protein
Ovis aries
5.3.99.3
6.5
7.5
IgG1(hei-7)-reactive protein
Ovis aries
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
GSH
2 mM required for maximal activity of IgG1(hei-7)-reactive protein; concentrations below 0.5 mM are required for maximal activity of IgG1(hei-26)-reactive protein; dependent on
Ovis aries
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.04
-
prostaglandin H2
IgG1(hei-7)-reactive protein
Ovis aries
5.3.99.3
0.15
-
prostaglandin H2
IgG1(hei-26)-reactive protein
Ovis aries
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
at least 2 different proteins with PGE-PGH isomerase activity
Ovis aries
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
17500
-
x * 17500, IgG1(hei-7)-reactive protein, SDS-PAGE
Ovis aries
5.3.99.3
180000
-
x * 180000, IgG1(hei-26)-reactive protein, SDS-PAGE
Ovis aries
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
-
Ovis aries
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.3
epithelium
at least 2 different proteins with PGE-PGH isomerase activity; epithelium cells
Ovis aries
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
3126
Ovis aries
Prostaglandin E2
-
3126
Ovis aries
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.3
?
x * 17500, IgG1(hei-7)-reactive protein, SDS-PAGE; x * 180000, IgG1(hei-26)-reactive protein, SDS-PAGE
Ovis aries
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.3
6.2
-
IgG1(hei-26)-reactive protein
Ovis aries
5.3.99.3
6.5
7.5
IgG1(hei-7)-reactive protein
Ovis aries