Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Decedue, C.J.; Broussard, E.A.; Larson, A.D.; Braymer, H.D.
    Purification and characterization of the extracellular proteinase of Serratia marcescens (1979), Biochim. Biophys. Acta, 569, 293-301.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 1,10-phenanthroline neither Ca2+, Zn2+ nor Fe2+ restores Serratia marcescens
3.4.24.40 EDTA Ca2+ (not) restores; Fe2+ (not); Zn2+ (not) Serratia marcescens
3.4.24.40 additional information
-
 Serratia marcescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.40 extracellular
-
 Serratia marcescens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.40 additional information
-
 amino acid composition Serratia marcescens
3.4.24.40 47500
-
 1 * 47500, Serratia marcescens, SDS-PAGE Serratia marcescens
3.4.24.40 51900
-
 Serratia marcescens, sedimentation equilibrium centrifugation Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Serratia marcescens
-
 derepressed strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40 to near homogeneity Serratia marcescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.40 culture supernatant
-
 Serratia marcescens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.40 additional information
-
-
 Serratia marcescens

Storage Stability

EC Number Storage Stability Organism
3.4.24.40 -70°C, lyophilized Serratia marcescens enzyme, several months Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 Aminoethylated lysozyme + H2O
-
 Serratia marcescens ?
-
 ?
3.4.24.40 C-terminal octapeptide of glucagon + H2O cleavage sites Serratia marcescens ?
-
 ?
3.4.24.40 Carboxymethyl-beta-insulin + H2O cleavage sites Serratia marcescens ?
-
 ?
3.4.24.40 casein + H2O
-
 Serratia marcescens ?
-
 ?
3.4.24.40 Glucagon + H2O cleavage sites Serratia marcescens ?
-
 ?
3.4.24.40 Hemoglobin + H2O
-
 Serratia marcescens ?
-
 ?
3.4.24.40 additional information no substrates are small peptides Serratia marcescens ?
-
 ?
3.4.24.40 additional information minimum peptide size: hexapeptide Serratia marcescens ?
-
 ?
3.4.24.40 additional information no esterase activity: no substrates are N-benzoyl-L-Arg ethyl ester or N-benzoyl-L-Tyr ethyl esters Serratia marcescens ?
-
 ?
3.4.24.40 N-terminal hexapeptide of glucagon + H2O cleavage sites Serratia marcescens ?
-
 ?
3.4.24.40 Ribonuclease + H2O
-
 Serratia marcescens ?
-
 ?
3.4.24.40 Tryptic insulin heptapeptide + H2O cleavage sites Serratia marcescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.40 monomer 1 * 47500, Serratia marcescens, SDS-PAGE Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.40 7.5 10.6
-
 Serratia marcescens