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Literature summary extracted from

  • Nakajima, M.; Mizusawa, K.; Yoshida, F.
    Purification and properties of an extracellular proteinase of psychrophilic Escherichia freundii (1974), Eur. J. Biochem., 44, 87-96.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.24.40 Unstable in the presence of 10 mM CaCl2 Citrobacter freundii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 1,10-phenanthroline 1 mM Citrobacter freundii
3.4.24.40 2,2'-bipyridyl 5 mM Citrobacter freundii
3.4.24.40 8-hydroxyquinoline 0.1 mM at pH 10, 5 mM at pH 7 Citrobacter freundii
3.4.24.40 benzalkonium chloride
-
 Citrobacter freundii
3.4.24.40 Cd2+ at pH 10, not at pH 7 Citrobacter freundii
3.4.24.40 EDTA 0.1 mM at pH 10, 5 mM at pH 7 Citrobacter freundii
3.4.24.40 Hg2+
-
 Citrobacter freundii
3.4.24.40 KCN at pH 10, no inhibition at pH 7.5 Citrobacter freundii
3.4.24.40 additional information 2-mercaptoethanol; diisopropyl phosphofluoridate; iodine, ovomucoid; monoiodoacetic acid; PCMB; potato inhibitor; soybean trypsin inhibitor Citrobacter freundii
3.4.24.40 N-bromosuccinimide strong Citrobacter freundii
3.4.24.40 Ni2+ at pH 10, not at pH 7 Citrobacter freundii
3.4.24.40 Sodium thioglycolate 1 mM, at pH 10, no inhibition at pH 7.5 Citrobacter freundii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.40 extracellular
-
 Citrobacter freundii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.40 Ca2+ requirement Citrobacter freundii
3.4.24.40 Co2+ activation Citrobacter freundii
3.4.24.40 Co2+ 1 mM, at pH 10, not at pH 7 Citrobacter freundii
3.4.24.40 additional information atomic absorption spectroscopy Citrobacter freundii
3.4.24.40 additional information magnesium, manganese, cobalt, copper or iron Citrobacter freundii
3.4.24.40 Zn2+ zinc-containing metalloproteinase Citrobacter freundii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.40 additional information
-
 amino acid composition Citrobacter freundii
3.4.24.40 45000
-
 Escherichia freundii, gel filtration Citrobacter freundii
3.4.24.40 45400
-
 Escherichia freundii, minimum MW calculated on the basis of zinc content Citrobacter freundii
3.4.24.40 45800
-
 Escherichia freundii, sedimentation equilibrium studies Citrobacter freundii
3.4.24.40 51000
-
 1 * 51000, Escherichia freundii, SDS-PAGE Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Citrobacter freundii
-
 psychrophilic strain B-3038
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.40 glycoprotein 0.69 mol sugar/mol enzyme Citrobacter freundii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40
-
 Citrobacter freundii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.40 culture supernatant
-
 Citrobacter freundii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.40 2174
-
-
 Citrobacter freundii

Storage Stability

EC Number Storage Stability Organism
3.4.24.40 0°C, pH 4.5-11.5, 1 day Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 casein + H2O
-
 Citrobacter freundii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.40 monomer 1 * 51000, Escherichia freundii, SDS-PAGE Citrobacter freundii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.40 25
-
 at pH 10, in sodium carbonate buffer Citrobacter freundii
3.4.24.40 40
-
 at pH 8, in phosphate buffer Citrobacter freundii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.40 20
-
 3 h, stable at pH 5-10 Citrobacter freundii
3.4.24.40 25
-
 and below, 10 min stable at pH 10 Citrobacter freundii
3.4.24.40 30
-
 1 h, stable at pH 6-8.5 Citrobacter freundii
3.4.24.40 35
-
 10 min, pH 10, inactivation Citrobacter freundii
3.4.24.40 45
-
 and below, 10 min stable at pH 6.5 Citrobacter freundii
3.4.24.40 55
-
 10 min, inactivation, CaCl2 does not stabilize Citrobacter freundii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.40 additional information
-
 pI: 5.2 Citrobacter freundii
3.4.24.40 10
-
-
 Citrobacter freundii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.40 7.2 10.8 about half-maximal activity at pH 7.2 and 10.8 Citrobacter freundii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.40 5 10 3 h, stable at 30°C Citrobacter freundii
3.4.24.40 6 8.5 1 h, stable at 30°C Citrobacter freundii
3.4.24.40 6.5
-
 10 min, stable below 45°C Citrobacter freundii
3.4.24.40 10
-
 10 min, stable below 25°C, inactivation at 35°C Citrobacter freundii