EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.36 | 1,10-phenanthroline | inactivation by dialysis against 1,10-phenanthroline, reactivation of apoenzyme by Zn2+, partially by Cu2+, Co2+, Cd2+ or Ni2+, not by Hg2+, Ca2+, Fe2+, Pb2+, Ba2+, Sn2+, Mg2+, Mn2+, Fe3+ or Ag+; strong, 1 mM, Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
3.4.24.36 | 2,2'-bipyridyl | strong, Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
3.4.24.36 | 2-mercaptoethylamine | Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
3.4.24.36 | 8-hydroxyquinoline | Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
3.4.24.36 | EDTA | strong, Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
3.4.24.36 | EGTA | strong, Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
3.4.24.36 | thioglycolic acid | Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.36 | cell surface | major surface protein | Leishmania amazonensis | 9986 | - |
3.4.24.36 | cell surface | major surface protein | Leishmania mexicana pifanoi | 9986 | - |
3.4.24.36 | membrane | - |
Leishmania amazonensis | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.36 | Zinc | zinc containing metalloproteinase | Leishmania amazonensis | |
3.4.24.36 | Zinc | zinc containing metalloproteinase | Leishmania mexicana pifanoi | |
3.4.24.36 | Zinc | amino acid sequence between 161-173 and 304-308 shows strong homology to zinc-binding sites with the histidine ligands and the glutamate ligand of other metalloproteinases | Leishmania amazonensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.36 | additional information | Leishmania amazonensis | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | ? | - |
? | |
3.4.24.36 | additional information | Leishmania mexicana pifanoi | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | ? | - |
? | |
3.4.24.36 | additional information | Leishmania amazonensis LV78 | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.36 | Leishmania amazonensis | - |
LV78 | - |
3.4.24.36 | Leishmania amazonensis LV78 | - |
LV78 | - |
3.4.24.36 | Leishmania mexicana pifanoi | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.24.36 | glycoprotein | - |
Leishmania amazonensis |
3.4.24.36 | glycoprotein | - |
Leishmania mexicana pifanoi |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.36 | promastigotes, monoclonal affinity purification | Leishmania amazonensis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.36 | amastigote | enzymes from promastigotes and amastigotes are not identical but immunologically related | Leishmania amazonensis | - |
3.4.24.36 | amastigote | enzymes from promastigotes and amastigotes are not identical but immunologically related | Leishmania mexicana pifanoi | - |
3.4.24.36 | amastigote | axenic amastigotes from Leishmania mexicana pifanoi grown under macrophage-free conditions | Leishmania mexicana pifanoi | - |
3.4.24.36 | promastigote | enzymes from promastigotes and amastigotes are not identical but immunologically related | Leishmania amazonensis | - |
3.4.24.36 | promastigote | enzymes from promastigotes and amastigotes are not identical but immunologically related | Leishmania mexicana pifanoi | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.36 | Bovine serum albumin + H2O | radioiodinated | Leishmania amazonensis | ? | - |
? | |
3.4.24.36 | Bovine serum albumin + H2O | radioiodinated | Leishmania mexicana pifanoi | ? | - |
? | |
3.4.24.36 | Bovine serum albumin + H2O | radioiodinated | Leishmania amazonensis LV78 | ? | - |
? | |
3.4.24.36 | Fibrinogen + H2O | - |
Leishmania amazonensis | ? | - |
? | |
3.4.24.36 | Fibrinogen + H2O | - |
Leishmania amazonensis LV78 | ? | - |
? | |
3.4.24.36 | Hemoglobin + H2O | - |
Leishmania amazonensis | ? | - |
? | |
3.4.24.36 | Hemoglobin + H2O | - |
Leishmania mexicana pifanoi | ? | - |
? | |
3.4.24.36 | Hemoglobin + H2O | - |
Leishmania amazonensis LV78 | ? | - |
? | |
3.4.24.36 | additional information | non-specific endopeptidase | Leishmania amazonensis | ? | - |
? | |
3.4.24.36 | additional information | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | Leishmania amazonensis | ? | - |
? | |
3.4.24.36 | additional information | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | Leishmania mexicana pifanoi | ? | - |
? | |
3.4.24.36 | additional information | non-specific endopeptidase | Leishmania amazonensis LV78 | ? | - |
? | |
3.4.24.36 | additional information | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | Leishmania amazonensis LV78 | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.36 | 37 | - |
assay at | Leishmania amazonensis |
3.4.24.36 | 37 | - |
assay at | Leishmania mexicana pifanoi |
3.4.24.36 | 60 | - |
- |
Leishmania amazonensis |
3.4.24.36 | 60 | - |
- |
Leishmania mexicana pifanoi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.36 | 4 | - |
promastigote enzyme | Leishmania amazonensis |