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Literature summary extracted from

  • Gagmann, M.L.; Geuss, U.; Fischer, S.; Kresse, G.B.
    Peptidyl-Asp metalloendopeptidase (1995), Methods Enzymol., 248, 782-787.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.33 acetonitrile slight activation at 5-10% v/v, inhibits at 1% Pseudomonas fragi

General Stability

EC Number General Stability Organism
3.4.24.33 Stable in up to 2 M urea, 0.01% w/v SDS or 0.1 M guanidine hydrochloride Pseudomonas fragi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.33 1,10-phenanthroline Zn2+ does not restore after prolonged incubation Pseudomonas fragi
3.4.24.33 acetonitrile 1% v/v, activation at 5-10% v/v Pseudomonas fragi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.33 extracellular
-
 Pseudomonas fragi
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.33 additional information data obtained from inhibition experiments support metalloproteinase character Pseudomonas fragi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.33 additional information
-
 partial amino acid sequences Pseudomonas fragi
3.4.24.33 27000
-
 Pseudomonas fragi, HPLC gel filtration Pseudomonas fragi

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.24.33 acetonitrile stable in 5-10% v/v acetonitrile Pseudomonas fragi

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.33 Pseudomonas fragi
-
 mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.33 to near homogeneity Pseudomonas fragi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.33 culture supernatant
-
 Pseudomonas fragi
-

Storage Stability

EC Number Storage Stability Organism
3.4.24.33 4°C, lyophilized in the presence of Tris-HCl buffer, pH 7.5, at least 2 years, 4 mg lyophilizate in 100 ml H2O, 2 weeks Pseudomonas fragi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.33 alpha-tubulin + H2O specific for preaspartate cleavage Pseudomonas fragi ?
-
 ?
3.4.24.33 Azocoll + H2O
-
 Pseudomonas fragi ?
-
 ?
3.4.24.33 casein + H2O resorufin-labeled casein Pseudomonas fragi ?
-
 ?
3.4.24.33 casein + H2O specific for preaspartate cleavage Pseudomonas fragi ?
-
 ?
3.4.24.33 Glucagon + H2O specific for preaspartate cleavage Pseudomonas fragi ?
-
 ?
3.4.24.33 Hemoglobin + H2O
-
 Pseudomonas fragi ?
-
 ?
3.4.24.33 Insulin A-chain + H2O
-
 Pseudomonas fragi ?
-
 ?
3.4.24.33 Insulin B-chain + H2O specific for preaspartate cleavage Pseudomonas fragi ?
-
 ?
3.4.24.33 Insulin B-chain + H2O also cleaves bonds with cysteic acid in P1' derived from cysteine residues by oxidation with performic acid and at N-terminal side of some glutamyl residues Pseudomonas fragi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.33 monomer 1 * 27000, Pseudomonas fragi, SDS-PAGE Pseudomonas fragi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.33 37
-
 assay at Pseudomonas fragi