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Literature summary extracted from

  • Mäkinen, P.L.; Clewell, D.B.; An, F.; Mäkinen, K.K.
    Purification and substrate specificity of a strongly hydrophobic extracellular metalloendopeptidase (gelatinase) from Streptococcus faecalis (strain 0G1-10) (1989), J. Biol. Chem., 264, 3325-3334.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.30 3,4,5,6-Tetrachloro-1,2-benzoquinone i.e. o-chloranil, 0.003 mM, most potent inactivator Enterococcus faecalis
3.4.24.30 diethylpyrocarbonate partially reversed by hydroxylamine (not) Enterococcus faecalis
3.4.24.30 ethanol
-
 Enterococcus faecalis
3.4.24.30 High MW factor of rat inflammatory exudate strong, MW: 720000-750000 Enterococcus faecalis
3.4.24.30 L-Leucine hydroxamate
-
 Enterococcus faecalis
3.4.24.30 methanol about 15% as effective as n-butanol Enterococcus faecalis
3.4.24.30 additional information no inhibition by 2,3-butanedinone; no inhibition by PMSF, phenylglyoxal Enterococcus faecalis
3.4.24.30 n-butanol
-
 Enterococcus faecalis
3.4.24.30 N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline 5 mM Enterococcus faecalis
3.4.24.30 phosphoramidon i.e. N-(alpha-L-rhamnopyranosyloxy-hydroxyphosphinyl)-L-Leu-L-Trp Enterococcus faecalis
3.4.24.30 Propanol
-
 Enterococcus faecalis
3.4.24.30 Tetranitromethane 5 mM, pH-dependent Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.30 0.02
-
 Substance P scissile bond Phe7-Phe8 Enterococcus faecalis
3.4.24.30 0.049
-
 Substance P scissile bond Gln6-Phe7 Enterococcus faecalis
3.4.24.30 0.0725
-
 Insulin B-chain scissile bond Phe24-Phe25 Enterococcus faecalis
3.4.24.30 0.12
-
 Substance P scissile bond Gly9-Leu10 Enterococcus faecalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.30 extracellular
-
 Enterococcus faecalis
-
-
3.4.24.30 soluble
-
 Enterococcus faecalis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.30 Zinc requirement, metalloendopeptidase Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.30 additional information
-
 amino acid composition shows similarity to Staphylococcus aureus metalloendopeptidase Enterococcus faecalis
3.4.24.30 29500
-
 Streptococcus faecalis, FPLC gel filtration Enterococcus faecalis
3.4.24.30 32000
-
 Streptococcus faecalis, gel filtration Enterococcus faecalis
3.4.24.30 33000
-
 1 * 33000, Streptococcus faecalis, SDS-PAGE Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.30 Enterococcus faecalis
-
 var. liquefaciens, strain OG1-10
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.30
-
 Enterococcus faecalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.30 culture filtrate
-
 Enterococcus faecalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.30 additional information
-
-
 Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.30 Ala-Leu-Ile-Leu-Thr-Leu-Val-Ser + H2O i.e. iPDI, competitive inhibitor of Streptococcus faecalis sex pheromones Enterococcus faecalis Ala-Leu-Ile-Leu-Thr + Leu-Val-Ser
-
 ?
3.4.24.30 angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu Enterococcus faecalis Asp-Arg-Val-Tyr + Ile-His-Pro + Phe-His-Leu
-
 ?
3.4.24.30 angiotensin II + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe Enterococcus faecalis Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
 ?
3.4.24.30 Azoalbumin + H2O bovine albumin, fraction V Enterococcus faecalis ?
-
 ?
3.4.24.30 azocasein + H2O
-
 Enterococcus faecalis ?
-
 ?
3.4.24.30 Azocoll + H2O general protease substrate Enterococcus faecalis ?
-
 ?
3.4.24.30 beta-Lipotropin cleavage site: Gly63-Phe64, poor substrate Enterococcus faecalis ?
-
 ?
3.4.24.30 Bovine casein-yellow + H2O
-
 Enterococcus faecalis ?
-
 ?
3.4.24.30 Bovine fibrinogen + H2O plasminogen-free or fraction I Enterococcus faecalis ?
-
 ?
3.4.24.30 Bovine hemoglobin + H2O
-
 Enterococcus faecalis ?
-
 ?
3.4.24.30 Bovine pancreas insulin + H2O poor substrate Enterococcus faecalis ?
-
 ?
3.4.24.30 bradykinin + H2O
-
 Enterococcus faecalis ?
-
 ?
3.4.24.30 Cholecystokinin + H2O cleavage site: Gly29-Trp30 Enterococcus faecalis ?
-
 ?
3.4.24.30 Collagen + H2O high or low viscose polypetides from collagen Enterococcus faecalis ?
-
 ?
3.4.24.30 Gelatin + H2O
-
 Enterococcus faecalis ?
-
 ?
3.4.24.30 Glucagon + H2O cleavage site: Trp25-Leu26 Enterococcus faecalis ?
-
 ?
3.4.24.30 Insulin A-chain + H2O cleavage at Gln15-Leu16 and Tyr14-Gln15, to a much lesser extent than B-chain Enterococcus faecalis Fragments of insulin A-chain peptides (Gln1-Gln15) and (Leu16-Asn21), another product formed: peptide (Gln1-Trp14) ?
3.4.24.30 Insulin B-chain + H2O oxidized, best substrate, preferred cleavage at Phe24-Phe25, followed by His5-Leu6, after prolonged incubation: Ala14-Leu15 and His10-Leu11 Enterococcus faecalis ?
-
 ?
3.4.24.30 Leu-Phe-Ser-Leu-Val-Leu-Ala-Gly + H2O i.e. cADI, Streptococcus faecalis sex pheromone, poor substrate Enterococcus faecalis Leu-Phe-Ser + Leu-Val-Leu-Ala-Gly
-
 ?
3.4.24.30 Leu-Phe-Val-Val-Thr-Leu-Val-Gly + H2O i.e. iADI, competitive inhibitor of Streptococcus faecalis sex pheromones Enterococcus faecalis Leu-Phe-Val-Val-Thr + Leu-Val-Gly
-
 ?
3.4.24.30 Leucine-enkephalin + H2O cleavage site: Gly3-Phe4, poor substrate Enterococcus faecalis ?
-
 ?
3.4.24.30 Lys-bradykinin + H2O
-
 Enterococcus faecalis ?
-
 ?
3.4.24.30 additional information strict specificity for hydrophobic amino acid at position P1', exhibits endopeptidase and thermolysin-like specificity Enterococcus faecalis ?
-
 ?
3.4.24.30 additional information very poor substrates are elastin-orcein or hide powder azure Enterococcus faecalis ?
-
 ?
3.4.24.30 additional information No substrates are Ala-Ile-Phe-Ile-Ala-Ser (i.e. Streptococcus faecalis sex pheromone cAM373), aminoacyl-2-naphthylamides, Nalpha-benzoyl-DL-Arg 4-nitroanilide, phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg, 2-furylacryloyl-L-Leu-Gly-L-Pro-L-Ala Enterococcus faecalis ?
-
 ?
3.4.24.30 N-3-(2-Furyl)acryloyl-Gly-L-Leu amide + H2O chromogenic substrate, cleavage site: Gly-Leu, poor substrate Enterococcus faecalis ?
-
 ?
3.4.24.30 neurotensin + H2O cleavage site: Tyr11-Ile12, poor substrate Enterococcus faecalis fragments of neurotensin peptides (Arg1-Gly9) and (Leu10-Met11) are the first occuring products ?
3.4.24.30 Phe-Leu-Val-Met-Phe-Leu-Ser-Gly + H2O i.e. cPDI, Streptococcus faecalis sex pheromone, poor substrate Enterococcus faecalis Phe-Leu-Val-Met-Phe + Leu-Ser-Gly
-
 ?
3.4.24.30 Substance P + H2O i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 Enterococcus faecalis Arg-Pro-Lys-Pro-Gln-Gln + Phe + Phe-Gly + Leu-Met-NH2
-
 ?
3.4.24.30 Substance P + H2O
-
 Enterococcus faecalis ?
-
 ?
3.4.24.30 [Lys1]bradykinin + H2O
-
 Enterococcus faecalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.30 monomer 1 * 33000, Streptococcus faecalis, SDS-PAGE Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.30 37
-
 assay at Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.30 additional information
-
 pI: 4.6 Enterococcus faecalis
3.4.24.30 6 8 broad, azocoll as substrate Enterococcus faecalis
3.4.24.30 7.2
-
 cleavage of Ser3-Leu4 in pheromone cAD1 Enterococcus faecalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.30 5.6
-
 above, 17 h stable in 50 mM 2-[N-morpholino]ethanesulfonic acid buffer Enterococcus faecalis