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Literature summary extracted from
- The purification and properties of a fibrinolytic neutral metalloendopeptidase from Streptococcus faecalis (1980), Arch. Biochem. Biophys., 202, 629-638.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.24.30 | Rapid autolysis, Zn2+ as protease inhibitor protects, Ca2+ does not stabilize | Enterococcus faecalis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.30 | 1,10-phenanthroline | - |
Enterococcus faecalis |  |
| 3.4.24.30 | diethylpyrocarbonate | partially reversed by hydroxylamine (not) | Enterococcus faecalis | |
| 3.4.24.30 | EDTA | reversible by Co2+, Mn2+, Zn2+, Ca2+, Cu2+ or Mg2+ with decreasing order of efficiency, not by Cd2+, Ba2+, Ni2+ or Hg2+ | Enterococcus faecalis | |
| 3.4.24.30 | L-cysteine | - |
Enterococcus faecalis | |
| 3.4.24.30 | additional information | no inhibition by DTNB, carbodiimide/glycine ethyl ester; no inhibition by iodoacetate; no inhibition by PMSF, phenylglyoxal; no inhibition by tosyl-Leu chloromethyl ketone, tosyl-Phe chloromethyl ketone, trasylol (i.e. bovine lung trypsin inhibitor) | Enterococcus faecalis | |
| 3.4.24.30 | NaBr | above 5 M, activates at 1-5 M | Enterococcus faecalis | |
| 3.4.24.30 | p-chloromercuribenzoate | not | Enterococcus faecalis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.30 | 5 | - |
N-3-(2-furyl)acryloylglycyl-L-leucinamide | - |
Enterococcus faecalis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.30 | extracellular | - |
Enterococcus faecalis | - |
- |
| 3.4.24.30 | soluble | - |
Enterococcus faecalis | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.30 | NaBr | activation, 1-5 M, inhibits above | Enterococcus faecalis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.30 | 30000 | - |
x * 30000, Streptococcus faecalis, SDS-PAGE | Enterococcus faecalis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.30 | Enterococcus faecalis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.30 | affinity chromatography | Enterococcus faecalis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.30 | culture filtrate | - |
Enterococcus faecalis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.30 | additional information | - |
- |
Enterococcus faecalis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.24.30 | At very low temperatures stored in the presence of Zn2+ as protease inhibitor to prevent autolysis of purified enzyme | Enterococcus faecalis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.30 | Bovine fibrinogen + H2O | B-chain and to a lesser extent A-chain | Enterococcus faecalis | ? | - |
? | |
| 3.4.24.30 | additional information | No substrates are N-3-(2-furyl)acryloyl-Gly-Gly-Gly, N-3-(2-furyl)acryloyl-Gly-L-Leu or N-3-(2-furyl)acryloyl-Gly-L-Ala | Enterococcus faecalis | ? | - |
? | |
| 3.4.24.30 | additional information | neutral metalloendopeptidase, no aminopeptidase or esterase activity | Enterococcus faecalis | ? | - |
? | |
| 3.4.24.30 | N-3-(2-Furyl)acryloyl tripeptides derivatives + H2O | chromogenic substrates, e.g. -L-Ala-L-Ala-L-Ala, -Gly-L-Phe-L-Phe, -L-Phe-L-Phe-L-Phe, -Gly-L-Leu-L-Tyr, -Gly-L-Met-L-Leu, -L-Leu-L-Leu-L-Leu | Enterococcus faecalis | ? | - |
? | |
| 3.4.24.30 | N-3-(2-Furyl)acryloyl-Gly-L-Leu amide + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| 3.4.24.30 | N-3-(2-Furyl)acryloyl-Gly-L-Phe + H2O | chromogenic substrate | Enterococcus faecalis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.30 | ? | x * 30000, Streptococcus faecalis, SDS-PAGE | Enterococcus faecalis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.30 | 25 | - |
spectrophotometric assay at | Enterococcus faecalis |
| 3.4.24.30 | 37 | - |
assay at | Enterococcus faecalis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.30 | additional information | - |
Ca2+ reduces thermal stability | Enterococcus faecalis |
| 3.4.24.30 | 55 | - |
rapid inactivation above | Enterococcus faecalis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.30 | additional information | - |
pI: 5 | Enterococcus faecalis |
| 3.4.24.30 | 6.5 | - |
N-3-(2-furyl)acryloyl-Gly-L-Leu amide as substrate | Enterococcus faecalis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.30 | 5 | 7.5 | about half-maximal activity at pH 5 and 7.5, N-3-(2-furyl)acryloyl-Gly-L-Leu amide as substrate | Enterococcus faecalis |
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