Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Morihara, K.
    Pseudolysin and other pathogen endopeptidases of thermolysin family (1995), Methods Enzymol., 248, 242-253.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.26
-
 Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.26 1,10-phenanthroline
-
 Pseudomonas aeruginosa
3.4.24.26 ClCH2CO-N-hydroxyleucine-Ala-Gly-NH2
-
 Pseudomonas aeruginosa
3.4.24.26 EDTA
-
 Pseudomonas aeruginosa
3.4.24.26 additional information diisopropyl fluorophosphate; not: ClCH2CO-N-hydroxyleucine-OCH3; tosyl-L-Phe chloromethyl ketone, PCMB Pseudomonas aeruginosa
3.4.24.26 peptides containing the hydroxamic acid, N-hydroxypeptide and thiol functional groups Pseudomonas aeruginosa
3.4.24.26 phosphoramidon
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.26 0.11
-
 2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzylamide
-
 Pseudomonas aeruginosa
3.4.24.26 0.11
-
 2-aminobenzoyl-Ala-Gly-Leu-Ala-4-nitrobenzylamide
-
 Vibrio cholerae serotype O1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.26 Zinc essential for activity Pseudomonas aeruginosa
3.4.24.26 Zinc contains zinc Pseudomonas aeruginosa
3.4.24.26 Zinc contains 1 atom Zn per molecule Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.26 33000
-
 Pseudomonas aeruginosa Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.26 Pseudomonas aeruginosa
-
-
-
3.4.24.26 Vibrio cholerae serotype O1
-
 similar enzyme produced by Vibrio cholerae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.26
-
 Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.26 culture medium
-
 Pseudomonas aeruginosa
-
3.4.24.26 culture medium
-
 Vibrio cholerae serotype O1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.26 2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzylamide + H2O
-
 Pseudomonas aeruginosa 2-Aminobenzoyl-Ala-Gly + Leu-Ala 4-nitrobenzylamide
-
 ?
3.4.24.26 2-aminobenzoyl-Ala-Gly-Leu-Ala-4-nitrobenzylamide + H2O
-
 Vibrio cholerae serotype O1 2-aminobenzoyl-Ala-Gly + Leu-Ala-4-nitrobenzylamide
-
 ?
3.4.24.26 casein + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 Denatured casein + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 Denatured fibrin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 Denatured hemoglobin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 Denatured ovalbumin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 Elastin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 additional information with synthetic substrates of the general structure benzyloxycarbonyl-Phe-Xaa-Ala, the Phe-Xaa bond is cleaved, the decreasing order of preference for Xaa is as follows: Phe, Leu, Tyr, Val, Ile Pseudomonas aeruginosa ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.26 70
-
 pH 7.0, 10 min, in presence of Ca2+ stable Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.26 100
-
 2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzylamide
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.26 7 8 casein, elastin Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.26 6 10 4°C, 16 h, in presence of Ca2+ stable Pseudomonas aeruginosa