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Literature summary extracted from

  • Okada, Y.; Morodomi, T.; Enghild, J.J.; Suzuki, K.; Yasui, A.; Nakanishi, I.; Salvesen, G.; Nagase, H.
    Matrix metalloproteinase 2 from human rheumatoid synovial fibroblasts. Purification and activation of the precursor and enzymic properties (1990), Eur. J. Biochem., 194, 721-730.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.24 additional information rapid activation of the zymogen by 4-aminophenylmercuric acetate Homo sapiens
3.4.24.24 additional information no activation by endopeptidases (trypsin, chymotrypsin, plasmin, plasma kallikrein, thrombin, neutrophil elastase, cathepsin G, matrix metalloproteinase 3, thermolysin) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.24 1,10-phenanthroline
-
 Homo sapiens
3.4.24.24 1,4-dithiothreitol
-
 Homo sapiens
3.4.24.24 EDTA
-
 Homo sapiens
3.4.24.24 glycine
-
 Homo sapiens
3.4.24.24 additional information not: inhibitors of serine, cysteine or aspartic proteinases Homo sapiens
3.4.24.24 SDS
-
 Homo sapiens
3.4.24.24 Tissue inhibitor of metalloproteinases
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.24 extracellular
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.24 67000
-
 x * 67000, human, active form, SDS-PAGE under reducing condition Homo sapiens
3.4.24.24 72000
-
 x * 72000, human, zymogen, SDS-PAGE under reducing conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.24 additional information Homo sapiens constitutively expressed in human rheumatoid synovial cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.24 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.24 as an inactive zymogen, rapid activation by 4-aminophenylmercuric acetate Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.24 culture medium from human rheumatoid synovial cells Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.24 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.24 Azocoll + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.24 Cartilage proteoglycan + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.24 Collagen + H2O type V Homo sapiens ?
-
 ?
3.4.24.24 Collagen + H2O type IV (to a lesser extent) Homo sapiens ?
-
 ?
3.4.24.24 Elastin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.24 Fibronectin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.24 Gelatin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.24 Laminin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.24 additional information constitutively expressed in human rheumatoid synovial cells Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.24 ? x * 72000, human, zymogen, SDS-PAGE under reducing conditions Homo sapiens
3.4.24.24 ? x * 67000, human, active form, SDS-PAGE under reducing condition Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.24 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.24 8.5
-
 azocoll Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.24 6 9 pH 6.0: about 40% of activity maximum, pH 9.0: about 85% of activity maximum, azocoll Homo sapiens