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Literature summary extracted from

  • Crabbe, T.; Willenbrock, F.; Eaton, D.; Hynds, P.; Carne, A.F.; Murphy, G.; Docherty, A.J.P.
    Biochemical characterization of matrilysin. Activation conforms to the stepwise mechanisms proposed for other matrix metalloproteinases (1992), Biochemistry, 31, 8500-8507.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.23
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.23 1,10-phenanthroline
-
 Homo sapiens
3.4.24.23 Tissue inhibitor of metalloproteinase-1
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.23 Zinc contains 1 zinc atom per molecule which is essential for catalytic acivity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.23 19000
-
 x * 19000, human, active form, SDS-PAGE under reducing condition Homo sapiens
3.4.24.23 28000
-
 x * 28000, human, proenzyme, SDS-PAGE under reducing condition Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.23 Homo sapiens
-
 expression in Escherichia coli
-
3.4.24.23 Homo sapiens
-
 expression by transfected mouse myeloma cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.23 latent precursor purified from transfected mouse myeloma cell conditioned medium, activation by 4-aminophenylmercuric acetate, trypsin and incubation at elevated temperatures Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.23 culture medium transfected mouse myeloma cell conditioned Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.23 2,4-Dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 + H2O
-
 Homo sapiens 2,4-Dinitrophenyl-Pro-Leu-Gly + Leu-Trp-Ala-D-Arg-NH2
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.23 ? x * 19000, human, active form, SDS-PAGE under reducing condition Homo sapiens
3.4.24.23 ? x * 28000, human, proenzyme, SDS-PAGE under reducing condition Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.23 6.5
-
 2,4-dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.23 4.5 8.5 4.5: about 40% of activity maximum, 8.5: about 65% of activity maximum, 2,4-dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg Homo sapiens