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Literature summary extracted from

  • Hojima, Y.; van der Rest, M.; Prockop, P.J.
    Type I procollagen carboxyl-terminal proteinase from chick embryo tendons. Purification and characterization (1985), J. Biol. Chem., 260, 15996-16003.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.24.19 Ca2+ stabilizes against thermal inactivation Gallus gallus
3.4.24.19 Lyophilization overnight, 50% loss of activity Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.19 (NH4)2SO4
-
 Gallus gallus
3.4.24.19 1,10-phenanthroline
-
 Gallus gallus
3.4.24.19 1,4-dithio-L-threitol
-
 Gallus gallus
3.4.24.19 2,2'-dipyridyl
-
 Gallus gallus
3.4.24.19 2-mercaptoethanol
-
 Gallus gallus
3.4.24.19 Bovine alpha2-macroglobulin
-
 Gallus gallus
3.4.24.19 Cd2+ CdCl2 Gallus gallus
3.4.24.19 Chicken alpha2-macroglobulin
-
 Gallus gallus
3.4.24.19 chymostatin
-
 Gallus gallus
3.4.24.19 CoCl2
-
 Gallus gallus
3.4.24.19 concanavalin A
-
 Gallus gallus
3.4.24.19 CuCl2
-
 Gallus gallus
3.4.24.19 diisopropylphosphofluoridate weak Gallus gallus
3.4.24.19 EDTA
-
 Gallus gallus
3.4.24.19 EGTA
-
 Gallus gallus
3.4.24.19 Fetal bovine serum weak Gallus gallus
3.4.24.19 glycine weak Gallus gallus
3.4.24.19 L-alanine weak Gallus gallus
3.4.24.19 L-arginine weak Gallus gallus
3.4.24.19 L-lysine
-
 Gallus gallus
3.4.24.19 metal chelators
-
 Gallus gallus
3.4.24.19 MnCl2
-
 Gallus gallus
3.4.24.19 additional information not: cysteine proteinases; not: inhibitors of serine Gallus gallus
3.4.24.19 Na2SO4
-
 Gallus gallus
3.4.24.19 Na3PO4
-
 Gallus gallus
3.4.24.19 NH4Cl weak Gallus gallus
3.4.24.19 NiCl2
-
 Gallus gallus
3.4.24.19 Ovostatin weak Gallus gallus
3.4.24.19 pepstatin A Gallus gallus
3.4.24.19 phosphoramidon weak Gallus gallus
3.4.24.19 PMSF weak Gallus gallus
3.4.24.19 reduced glutathione
-
 Gallus gallus
3.4.24.19 SDS
-
 Gallus gallus
3.4.24.19 ZnCl2
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.19 0.000096
-
 Procollagen type I
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.19 Ca2+ dependent on, 5-10 mM required for optimal activity Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.19 97000 110000 gel filtration, PAGE Gallus gallus
3.4.24.19 102000
-
 1 * 102000, chicken, SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.19 Procollagen type I + H2O Gallus gallus enzyme is involved in the processing of type I procollagen ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.19 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.19
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.19 culture medium of 17-day-old embryo tendons Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.19 additional information
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
3.4.24.19 4°C, -20°C or -70°C in 0.3 M Tris-HCl, 1.5 M NaCl, 5 mM CaCl2, 0.02% Brij 35, 0.01% NaN3, pH 7.5, less than 25% loss of activity after 2 months Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.19 Procollagen type I + H2O specifically cleaves an Ala-Asp bond in both the pro-alpha1(I) and pro-alpha2(I) chains Gallus gallus Type I pNcollagen + C-propeptides
-
 ?
3.4.24.19 Procollagen type I + H2O specifically cleaves carboxyl-terminal propeptides of a homotrimer of pro-alpha1(I) chains and type II and III procollagens, but it does not cleave type IV procollagen Gallus gallus Type I pNcollagen + C-propeptides
-
 ?
3.4.24.19 Procollagen type I + H2O enzyme is involved in the processing of type I procollagen Gallus gallus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.19 monomer 1 * 102000, chicken, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.19 35
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.19 8 8.5
-
 Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.19 7.5 8.5 7.5: about 80% of activity maximum, 8-8.5: activity maximum Gallus gallus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.19 4.3
-
 1 h, in presence of 5 mM CaCl2, more than 80% loss of activity Gallus gallus
3.4.24.19 4.8
-
 1 h, in presence of 5 mM CaCl2, more than 80% loss of activity Gallus gallus
3.4.24.19 6 9 1 h, in presence of 5 mM CaCl2, less than 20% loss of activity Gallus gallus