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Literature summary extracted from

  • Butler, P.E.; McKay, M.J.; Bond, J.S.
    Characterization of meprin, a membrane-bound metalloendopeptidase from mouse kidney (1987), Biochem. J., 241, 229-235.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.18 1,10-phenanthroline at neutral and alkaline pH-values, reversible by dialysis Mus musculus
3.4.24.18 EDTA at neutral and alkaline pH-values, reversible by dialysis Mus musculus
3.4.24.18 additional information
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.18 membrane intrinsic protein Mus musculus 16020
-
3.4.24.18 membrane renal brush border membrane Mus musculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.18 Calcium metalloendopeptidase Mus musculus
3.4.24.18 Calcium 3 mol calcium per mol subunit (atomic absorption spectroscopy) Mus musculus
3.4.24.18 Calcium tightly bound, no addition of Ca2+ required Mus musculus
3.4.24.18 Zinc metalloendopeptidase Mus musculus
3.4.24.18 Zinc 1 mol zinc per mol subunit (atomic absorption spectroscopy) Mus musculus
3.4.24.18 Zinc tightly bound, no addition of Zn2+ required Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.18 additional information
-
 amino acid composition Mus musculus
3.4.24.18 85000
-
 4 * 85000, mouse Mus musculus
3.4.24.18 320000
-
 mouse, gel filtration, SDS-PAGE in the absence of 2-mercaptoethanol Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.18 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.18 glycoprotein about 18% carbohydrates, mostly N-linked sugars Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.18
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.18 kidney
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.18 angiotensin II + H2O poor substrate Mus musculus Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
 ?
3.4.24.18 angiotensin II + H2O cleavage site: Tyr-Ile Mus musculus Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
 ?
3.4.24.18 angiotensin II + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe Mus musculus Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
 ?
3.4.24.18 azocasein + H2O
-
 Mus musculus fragments of azocasein
-
 ?
3.4.24.18 bradykinin + H2O one cleavage site: Phe-Ser Mus musculus Arg-Pro-Pro-Gly + Phe-Ser-Pro-Phe-Arg
-
 ?
3.4.24.18 Insulin B-chain + H2O the others are Leu6-Cys(SO3-)7, Ala14-Leu15, Gly8-Ser9, His10-Leu11, Leu15-Tyr16, His5-Leu6 and Leu17-Val18 Mus musculus Hydrolyzed insulin B-chain 13 different peptide fragments ?
3.4.24.18 Insulin B-chain + H2O oxidized form Mus musculus Hydrolyzed insulin B-chain 13 different peptide fragments ?
3.4.24.18 additional information no hydrolysis of Leu 4-nitroanilide, benzoyl-Arg-4-nitroanilide, succinyl-Ala 4-nitroanilide, Arg 4-methylcoumarinin 7-amide, benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide, Gly-Gly-Phe-Leu, Tyr-Gly-Gly-Phe-Leu, Tyr-Gly-Gly-Phe-Met, Leu-Arg-Arg-Ala-Ser-Leu-Gly, Ala-Phe-Pro-Leu-Gly-Phe, benzoyl-Phe-Val-Arg Mus musculus ?
-
 ?
3.4.24.18 additional information hydrolyzes peptides of at least 8 amino acids and prefers peptide bonds flanked by hydrophobic or neutral amino acid residues although hydrolysis is not limited to these bonds Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.18 More dimeric meprin seems to be catalytically active Mus musculus
3.4.24.18 tetramer SDS-PAGE Mus musculus
3.4.24.18 tetramer 4 * 85000, mouse Mus musculus
3.4.24.18 tetramer in the presence of 2-mercaptoethanol Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.18 37
-
 assay at Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.18 60
-
 t1/2: 30 min at pH 7.5 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.18 additional information
-
 pI: multiple bonds in the range of 4-5, presumably due to glycosylation Mus musculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.18 7
-
 below, rapid irreversible denaturation Mus musculus
3.4.24.18 7.5
-
 t1/2: 30 min at 60°C Mus musculus