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Literature summary extracted from
- Purification and characterization of a metallo-endoproteinase from mouse kidney (1981), Biochem. J., 199, 591-598.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.11 | 1,10-phenanthroline | - |
Mus musculus |  |
| 3.4.24.11 | 2-mercaptoethanol | - |
Mus musculus | |
| 3.4.24.11 | chymostatin | - |
Mus musculus | |
| 3.4.24.11 | EDTA | - |
Mus musculus | |
| 3.4.24.11 | EGTA | - |
Mus musculus | |
| 3.4.24.11 | L-Cys | - |
Mus musculus | |
| 3.4.24.18 | 1,10-phenanthroline | - |
Mus musculus | |
| 3.4.24.18 | 2-mercaptoethanol | - |
Mus musculus | |
| 3.4.24.18 | chymostatin | - |
Mus musculus | |
| 3.4.24.18 | cysteine | not cystine | Mus musculus | |
| 3.4.24.18 | dithioerythritol | - |
Mus musculus | |
| 3.4.24.18 | EDTA | - |
Mus musculus | |
| 3.4.24.18 | EGTA | - |
Mus musculus | |
| 3.4.24.18 | GSH | not oxidized form | Mus musculus | |
| 3.4.24.18 | additional information | diisopropyl phosphofluoridate, p-hydroxymercuribenzoate, phenylmercurisulfonate, antipain, cystine, oxidized glutathione; iodoacetate; leupeptin; no inhibition by phosphoramidon; PMSF, pepstatin | Mus musculus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.18 | membrane | - |
Mus musculus | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.18 | additional information | considerable stimulation of azocasein hydrolysis at ionic strength values up to 0.15-0.2 | Mus musculus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.11 | 81000 | - |
probably 4 * 81000, SDS-PAGE in presence of 2-mercaptoethanol | Mus musculus |
| 3.4.24.11 | 270000 | 320000 | gel filtration | Mus musculus |
| 3.4.24.18 | 81000 | - |
x * 81000, mouse, SDS-PAGE in the presence of 2-mercaptoethanol | Mus musculus |
| 3.4.24.18 | 270000 | 320000 | mouse, gel filtration | Mus musculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.11 | Mus musculus | - |
- |
- |
| 3.4.24.18 | Mus musculus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.24.11 | side-chain modification | glycoprotein | Mus musculus |
| 3.4.24.18 | glycoprotein | - |
Mus musculus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.18 | solubilized from 100000 g sediment with toluene and trypsin | Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.11 | kidney | - |
Mus musculus | - |
| 3.4.24.18 | kidney | - |
Mus musculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.11 | Aldolase + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.11 | azocasein + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.11 | Azocoll + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.11 | benzyloxycarbonyl-Phe-Arg-4-methyl-7-coumarylamide + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.11 | casein + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.11 | haemoglobin + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.11 | Leu-2-naphthylamide + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.18 | Aldolase + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.18 | azocasein + H2O | - |
Mus musculus | fragments of azocasein | - |
? | |
| 3.4.24.18 | Azocoll + H2O | poor substrate | Mus musculus | ? | - |
? | |
| 3.4.24.18 | Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.18 | casein + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.18 | Hemoglobin + H2O | poor substrate | Mus musculus | ? | - |
? | |
| 3.4.24.18 | L-Leu 2-naphthylamide + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.24.18 | additional information | little or no activity towards benzoylarginine 2-naphthylamide, benzoylglycylarginine, benzyloxycarbonyl-Glu-Tyr, acetyl-Phe 2-naphthylester | Mus musculus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.11 | tetramer | probably 4 * 81000, SDS-PAGE in presence of 2-mercaptoethanol | Mus musculus |
| 3.4.24.18 | oligomer | x * 81000, mouse, SDS-PAGE in the presence of 2-mercaptoethanol | Mus musculus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.18 | 37 | - |
assay at | Mus musculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.11 | 9.5 | - |
hydrolysis of azocasein | Mus musculus |
| 3.4.24.18 | additional information | - |
pI: 4.3 | Mus musculus |
| 3.4.24.18 | 9.5 | - |
azocasein as substrate | Mus musculus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.11 | 7 | 10.3 | pH 7.0: about 40% of maximal activity, pH 10.3: about 25% of maximal activity | Mus musculus |
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