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Literature summary extracted from
- Human stromelysins 1 and 2 (1995), Methods Enzymol., 248, 449-470.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.22 | - |
Homo sapiens |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.24.17 | Ca2+ is required to maintain the active conformation, full activity is detected at 1 mM Ca2+, at pH 7.5 and is retained at even higher concentrations of Ca2+, at lower concentrations the enzyme is autolyzed | Homo sapiens |
| 3.4.24.17 | Ca2+ stabilizes | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.17 | 1,10-phenanthroline | - |
Homo sapiens |  |
| 3.4.24.17 | 1,10-phenanthroline | - |
Oryctolagus cuniculus | |
| 3.4.24.17 | Alpha-macroglobulin | - |
Homo sapiens | |
| 3.4.24.17 | cysteine | - |
Homo sapiens | |
| 3.4.24.17 | DTT | - |
Homo sapiens | |
| 3.4.24.17 | DTT | - |
Oryctolagus cuniculus | |
| 3.4.24.17 | EDTA | - |
Bos taurus | |
| 3.4.24.17 | EDTA | - |
Homo sapiens | |
| 3.4.24.17 | EDTA | - |
Oryctolagus cuniculus | |
| 3.4.24.17 | EDTA | - |
Rattus norvegicus | |
| 3.4.24.17 | EGTA | - |
Homo sapiens | |
| 3.4.24.17 | HONH-CO-CH2-CH(n-pentyl)-CO-Leu-Phe-NH2 | - |
Homo sapiens | |
| 3.4.24.17 | Ovostatin | - |
Homo sapiens | |
| 3.4.24.17 | Phthaloyl-N-(CH2)4-P(O2-)-Ile-(2-naphthyl)-Ala-NH-CH3 | - |
Homo sapiens | |
| 3.4.24.17 | Tissue inhibitor of metalloproteinases | TIMP-1 and TIMP-2 | Homo sapiens | |
| 3.4.24.22 | 1,10-phenanthroline | - |
Homo sapiens | |
| 3.4.24.22 | DTT | - |
Homo sapiens | |
| 3.4.24.22 | EDTA | - |
Homo sapiens | |
| 3.4.24.22 | EGTA | - |
Homo sapiens | |
| 3.4.24.22 | Specific tissue inhibitor of metalloproteinases | TIMP-1 | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.17 | 0.025 | - |
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
Homo sapiens | |
| 3.4.24.17 | 0.1 | - |
2,4-Dinitrophenyl-Pro-Tyr-Ala-Tyr-Trp-Met-Arg-NH2 | - |
Homo sapiens | |
| 3.4.24.22 | 0.023 | - |
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.17 | Ca2+ | Ca2+ is required to maintain the active conformation of the enzyme, full activity is detected at 1 mM Ca2+, at pH 7.5 and is retained at even higher concentrations of Ca2+, at lower concentrations the enzyme is autolyzed | Homo sapiens | |
| 3.4.24.17 | Ca2+ | Ca2+ is required to maintain the active conformation of the enzyme, full activity is detected at 1 mM Ca2+, at pH 7.5 and is retained at even higher concentrations of Ca2+, at lower concentrations the enzyme is autolyzed | Rattus norvegicus | |
| 3.4.24.17 | Ca2+ | Ca2+ is required to maintain the active conformation of the enzyme, full activity is detected at 1 mM Ca2+, at pH 7.5 and is retained at even higher concentrations of Ca2+, at lower concentrations the enzyme is autolyzed | Bos taurus | |
| 3.4.24.17 | Ca2+ | Ca2+ is required to maintain the active conformation of the enzyme, full activity is detected at 1 mM Ca2+, at pH 7.5 and is retained at even higher concentrations of Ca2+, at lower concentrations the enzyme is autolyzed | Oryctolagus cuniculus | |
| 3.4.24.17 | Ca2+ | proenzyme contains 3 Ca2+ | Homo sapiens | |
| 3.4.24.17 | Ca2+ | proenzyme contains 3 Ca2+ | Rattus norvegicus | |
| 3.4.24.17 | Ca2+ | proenzyme contains 3 Ca2+ | Bos taurus | |
| 3.4.24.17 | Ca2+ | proenzyme contains 3 Ca2+ | Oryctolagus cuniculus | |
| 3.4.24.17 | Zinc | zinc metalloprotein, proenzyme contains two Zn2+, the Zn2+ at the active site is bound to His-201, His-205, and His-211 and Cys-75 in the propeptide | Homo sapiens | |
| 3.4.24.22 | Zinc | zinc metalloenzyme | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.17 | additional information | - |
stromelysin 1 is synthesized as a preproenzyme of 477 amino acids and is secreted from cells as a proenzyme of 460 amino acids which consist of the propeptide (82 amino acids), the catalytic domain (165 amino acids), the proline-rich region (25 amino acids), and the C-terminal domain (188 amino acids) | Homo sapiens |
| 3.4.24.17 | additional information | - |
stromelysin 1 is synthesized as a preproenzyme of 477 amino acids and is secreted from cells as a proenzyme of 460 amino acids which consist of the propeptide (82 amino acids), the catalytic domain (165 amino acids), the proline-rich region (25 amino acids), and the C-terminal domain (188 amino acids) | Oryctolagus cuniculus |
| 3.4.24.22 | additional information | - |
synthesized as preproenzyme of 476 amino acids and secreted from cells as the proenzyme | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.22 | additional information | Homo sapiens | participates in activation of procollagenase proMMP-1 | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.17 | Bos taurus | - |
- |
- |
| 3.4.24.17 | Homo sapiens | - |
- |
- |
| 3.4.24.17 | Oryctolagus cuniculus | - |
- |
- |
| 3.4.24.17 | Rattus norvegicus | - |
- |
- |
| 3.4.24.22 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.24.22 | additional information | potential glycosylation site at Asn100, but is unlikely to be a glycoprotein as it does not bind concanavalin A | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.17 | - |
Homo sapiens |
| 3.4.24.22 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.17 | cartilage | - |
Homo sapiens | - |
| 3.4.24.17 | cartilage | - |
Rattus norvegicus | - |
| 3.4.24.17 | cartilage | - |
Bos taurus | - |
| 3.4.24.17 | cartilage | - |
Oryctolagus cuniculus | - |
| 3.4.24.17 | culture medium | synovial fibroblasts | Homo sapiens | - |
| 3.4.24.17 | culture medium | synovial fibroblasts | Rattus norvegicus | - |
| 3.4.24.17 | culture medium | synovial fibroblasts | Bos taurus | - |
| 3.4.24.17 | culture medium | synovial fibroblasts | Oryctolagus cuniculus | - |
| 3.4.24.17 | additional information | the enzyme is not readily detected in normal cells in culture or tissue homogenates, but its synthesis is greatly enhanced in mesenchymal cells when they are treated with cytokines such as interleukin-1 and tumor necrosis factor alpha, growth factors, phorbol myristate, acetate and several other stimuli | Homo sapiens | - |
| 3.4.24.17 | additional information | the enzyme is not readily detected in normal cells in culture or tissue homogenates, but its synthesis is greatly enhanced in mesenchymal cells when they are treated with cytokines such as interleukin-1 and tumor necrosis factor alpha, growth factors, phorbol myristate, acetate and several other stimuli | Rattus norvegicus | - |
| 3.4.24.17 | additional information | the enzyme is not readily detected in normal cells in culture or tissue homogenates, but its synthesis is greatly enhanced in mesenchymal cells when they are treated with cytokines such as interleukin-1 and tumor necrosis factor alpha, growth factors, phorbol myristate, acetate and several other stimuli | Bos taurus | - |
| 3.4.24.17 | additional information | the enzyme is not readily detected in normal cells in culture or tissue homogenates, but its synthesis is greatly enhanced in mesenchymal cells when they are treated with cytokines such as interleukin-1 and tumor necrosis factor alpha, growth factors, phorbol myristate, acetate and several other stimuli | Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.17 | additional information | - |
assay methods | Homo sapiens |
| 3.4.24.17 | additional information | - |
assay methods | Rattus norvegicus |
| 3.4.24.17 | additional information | - |
assay methods | Bos taurus |
| 3.4.24.17 | additional information | - |
assay methods | Oryctolagus cuniculus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.24.17 | -20°C, 50 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% w/v NaN3, 0.05% w/v Brij 35, stable for at least 1 year, prostromelysin 1 | Homo sapiens |
| 3.4.24.17 | 4°C, 50 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% w/v NaN3, 0.05% w/v Brij 35, stable for at least 4 months, prostromelysin 1 | Homo sapiens |
| 3.4.24.22 | -20°C, prostromelysin 2, 50 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% w/v NaN3, 0.05% Brij 35, stable for 4 weeks | Homo sapiens |
| 3.4.24.22 | 4°C, prostromelysin 2, 50 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% w/v NaN3, 0.05% Brij 35, stable for 2 weeks | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.17 | (7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 + H2O | - |
Homo sapiens | (7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu + norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
? | |
| 3.4.24.17 | (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 + H2O | - |
Homo sapiens | (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly + Leu-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 | - |
? | |
| 3.4.24.17 | 2,4-Dinitrophenyl-Arg-Pro-Lys-Pro-Leu-Ala-norvaline-Trp-NH2 + H2O | - |
Homo sapiens | 2,4-Dinitrophenyl-Arg-Pro-Lys-Pro-Leu-Ala + norvaline-Trp-NH2 | - |
? | |
| 3.4.24.17 | 2,4-Dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 + H2O | - |
Homo sapiens | 2,4-Dinitrophenyl-Pro-Leu-Gly + Leu-Trp-Ala-D-Arg-NH2 | - |
? | |
| 3.4.24.17 | 2,4-Dinitrophenyl-Pro-Tyr-Ala-Tyr-Trp-Met-Arg-NH2 + H2O | - |
Homo sapiens | 2,4-Dinitrophenyl-Pro-Tyr-Ala + Tyr-Trp-Met-Arg-NH2 | - |
? | |
| 3.4.24.17 | Aggrecan core protein + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.24.17 | casein + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.24.17 | casein + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.24.17 | Collagen type IV + H2O | - |
Homo sapiens | More | - |
? | |
| 3.4.24.17 | Collagen type IV + H2O | - |
Oryctolagus cuniculus | More | - |
? | |
| 3.4.24.17 | Elastin + H2O | limited activity | Homo sapiens | ? | - |
? | |
| 3.4.24.17 | Elastin + H2O | limited activity | Rattus norvegicus | ? | - |
? | |
| 3.4.24.17 | Elastin + H2O | limited activity | Bos taurus | ? | - |
? | |
| 3.4.24.17 | Elastin + H2O | limited activity | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.24.17 | Fibronectin + H2O | - |
Homo sapiens | Intact fibronectin subunit MW 22000 + a disulfide-bonded COOH-terminal MW 20000 polypeptide | - |
? | |
| 3.4.24.17 | Fibronectin + H2O | - |
Oryctolagus cuniculus | Intact fibronectin subunit MW 22000 + a disulfide-bonded COOH-terminal MW 20000 polypeptide | - |
? | |
| 3.4.24.17 | Gelatin + H2O | type 1 | Homo sapiens | ? | - |
? | |
| 3.4.24.17 | Gelatin + H2O | type 1 | Rattus norvegicus | ? | - |
? | |
| 3.4.24.17 | Gelatin + H2O | type 1 | Bos taurus | ? | - |
? | |
| 3.4.24.17 | Gelatin + H2O | type 1 | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.24.17 | additional information | activates procollagenase | Homo sapiens | ? | - |
? | |
| 3.4.24.17 | additional information | activates procollagenase | Rattus norvegicus | ? | - |
? | |
| 3.4.24.17 | additional information | activates procollagenase | Bos taurus | ? | - |
? | |
| 3.4.24.17 | additional information | activates procollagenase | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.24.17 | additional information | activates progelatinase B | Homo sapiens | ? | - |
? | |
| 3.4.24.17 | additional information | activates progelatinase B | Rattus norvegicus | ? | - |
? | |
| 3.4.24.17 | additional information | activates progelatinase B | Bos taurus | ? | - |
? | |
| 3.4.24.17 | additional information | activates progelatinase B | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.24.17 | additional information | enzyme is secreted from the cells as an inactive zymogen | Homo sapiens | ? | - |
? | |
| 3.4.24.17 | additional information | enzyme is secreted from the cells as an inactive zymogen | Rattus norvegicus | ? | - |
? | |
| 3.4.24.17 | additional information | enzyme is secreted from the cells as an inactive zymogen | Bos taurus | ? | - |
? | |
| 3.4.24.17 | additional information | enzyme is secreted from the cells as an inactive zymogen | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.24.17 | additional information | degrades a number of extracellular matrix components | Homo sapiens | ? | - |
? | |
| 3.4.24.17 | additional information | degrades a number of extracellular matrix components | Rattus norvegicus | ? | - |
? | |
| 3.4.24.17 | additional information | degrades a number of extracellular matrix components | Bos taurus | ? | - |
? | |
| 3.4.24.17 | additional information | degrades a number of extracellular matrix components | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.24.17 | additional information | participates in activation of procollagenases and progelatinase B | Homo sapiens | ? | - |
? | |
| 3.4.24.17 | additional information | participates in activation of procollagenases and progelatinase B | Rattus norvegicus | ? | - |
? | |
| 3.4.24.17 | additional information | participates in activation of procollagenases and progelatinase B | Bos taurus | ? | - |
? | |
| 3.4.24.17 | additional information | participates in activation of procollagenases and progelatinase B | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.24.17 | additional information | cleavage sites in natural proteins | Homo sapiens | ? | - |
? | |
| 3.4.24.17 | additional information | cleavage sites in natural proteins | Rattus norvegicus | ? | - |
? | |
| 3.4.24.17 | additional information | cleavage sites in natural proteins | Bos taurus | ? | - |
? | |
| 3.4.24.17 | additional information | cleavage sites in natural proteins | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.24.22 | (7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 + H2O | - |
Homo sapiens | (7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu + norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
? | |
| 3.4.24.22 | Aggrecan core protein + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.24.22 | Azocoll + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.24.22 | Cartilage link protein + H2O | cleaved at His16-Ile17 bond | Homo sapiens | ? | - |
? | |
| 3.4.24.22 | Collagen + H2O | type IV | Homo sapiens | ? | - |
? | |
| 3.4.24.22 | Elastin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.24.22 | Fibronectin + H2O | negligible | Homo sapiens | ? | - |
? | |
| 3.4.24.22 | Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.24.22 | additional information | participates in activation of procollagenase proMMP-1 | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.17 | 37 | - |
assay at | Homo sapiens |
| 3.4.24.17 | 37 | - |
assay at | Rattus norvegicus |
| 3.4.24.17 | 37 | - |
assay at | Bos taurus |
| 3.4.24.17 | 37 | - |
assay at | Oryctolagus cuniculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.17 | 37 | - |
48 h, stable, prostromelysin | Homo sapiens |
| 3.4.24.17 | 80 | - |
15 min, 35% loss of activity | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.17 | 0.24 | - |
2,4-Dinitrophenyl-Pro-Tyr-Ala-Tyr-Trp-Met-Arg-NH2 | - |
Homo sapiens | |
| 3.4.24.17 | 5.4 | - |
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
Homo sapiens | |
| 3.4.24.22 | 3.8 | - |
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.17 | 5.3 | 5.5 | digestion of aggrecan core protein | Homo sapiens |
| 3.4.24.22 | 5.5 | - |
activity against aggrecan at pH 5.5 is double that at pH 6.0 | Homo sapiens |
| 3.4.24.22 | 7.5 | 8 | azocoll | Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.22 | 6 | 8 | 6.0: 25% of activity maximum, 7.5-8.0: activity maximum, azocoll | Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.17 | 4.5 | - |
more than 80% loss of activity after 15 h | Homo sapiens |
| 3.4.24.17 | 5 | - |
about 50% loss of activity after 15 h | Homo sapiens |
| 3.4.24.17 | 5.5 | 9.5 | stable in this range | Homo sapiens |
| 3.4.24.17 | 10 | - |
about 50% loss of activity after 15 h | Homo sapiens |
| 3.4.24.17 | 10.5 | - |
more than 80% loss of activity after 15 h | Homo sapiens |
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