EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.17 | 1,10-phenanthroline | - |
Homo sapiens | |
3.4.24.17 | EDTA | - |
Homo sapiens | |
3.4.24.17 | additional information | not: inhibitors for serine, cysteine or aspartic proteinases | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.17 | extracellular | - |
Homo sapiens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.17 | Ca2+ | removal of Ca2+ from the enzyme solution results in a complete loss of activity that can be fully restored by addition of 1 mM Ca2+ | Homo sapiens | |
3.4.24.17 | Co2+ | addition of 0.5 mM Co2+ in the presence of Ca2+ restores activity of the apoenzyme, not by Co2+ alone | Homo sapiens | |
3.4.24.17 | Mn2+ | addition of 5 mM Mn2+ in the presence of Ca2+ restores activity of the apoenzyme, not by Mn2+ alone | Homo sapiens | |
3.4.24.17 | Zinc | Zn2+: addition of 0.5 mM Zn2+ in the presence of Ca2+ restores activity of the apoenzyme, not by Zn2+ alone | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.17 | additional information | - |
human, latent precursor, MW 51000, gel filtration | Homo sapiens |
3.4.24.17 | 28000 | - |
x * 28000, human, form LMW, SDS-PAGE | Homo sapiens |
3.4.24.17 | 45000 | - |
x * 45000, human, form HMW, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.17 | additional information | Homo sapiens | may play a role in the normal turnover of the connective tissue matrix as well as in the joint destruction of chronic synovitis | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.17 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.17 | 2 active forms: HMW, LMW | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.17 | culture medium | of rheumatoid synovial cells | Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.17 | additional information | - |
- |
Homo sapiens |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.24.17 | -20°C, 0°C or 4°C, 50 mM Tris-HCl, pH 7.5, 0.4 M NaCl, 10 mM Ca2+, 0.05% Brij 35, 0.02% NaN3, less than 19% loss of activity after 10 weeks | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.17 | Collagen type IV + H2O | - |
Homo sapiens | More | 4 major fragments of MW 165000, 145000, 125000 and 110000 | ? | |
3.4.24.17 | Fibronectin + H2O | - |
Homo sapiens | Intact fibronectin subunit MW 22000 + a disulfide-bonded COOH-terminal MW 20000 polypeptide | - |
? | |
3.4.24.17 | Gelatin + H2O | type 1 | Homo sapiens | ? | - |
? | |
3.4.24.17 | Laminin + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.24.17 | additional information | may play a role in the normal turnover of the connective tissue matrix as well as in the joint destruction of chronic synovitis | Homo sapiens | ? | - |
? | |
3.4.24.17 | Procollagen + H2O | removal of the NH2-terminal propeptides | Homo sapiens | ? | - |
? | |
3.4.24.17 | Procollagen + H2O | chicken type I | Homo sapiens | ? | - |
? | |
3.4.24.17 | Proteoglycan + H2O | cartilage | Homo sapiens | More | degradation to 12.0 S fragments by the HMW form and 10.3 S fragments by the KMW enzyme | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.17 | ? | x * 45000, human, form HMW, SDS-PAGE | Homo sapiens |
3.4.24.17 | ? | x * 28000, human, form LMW, SDS-PAGE | Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.17 | 80 | - |
15 min: 35% loss activity of enzyme form HMW, 5% loss of sctivity of enzyme form LMW | Homo sapiens |
3.4.24.17 | 100 | - |
15 min, 65% loss of activity of enzyme form HMW, 25% loss of activity of enzyme form LMW | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.17 | 7.5 | 7.8 | - |
Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.17 | 6 | 8.5 | about 50% of activity maximum at pH 6.0 and 8.5 | Homo sapiens |