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Literature summary extracted from

  • Okada, Y.; Nagase, H.; Harris, E.D.
    A metalloproteinase from human rheumatoid synovial fibroblasts that digests connective tissue matrix components. Purification and characterization (1986), J. Biol. Chem., 261, 14245-14255.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.17 1,10-phenanthroline
-
Homo sapiens
3.4.24.17 EDTA
-
Homo sapiens
3.4.24.17 additional information not: inhibitors for serine, cysteine or aspartic proteinases Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.17 extracellular
-
Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.17 Ca2+ removal of Ca2+ from the enzyme solution results in a complete loss of activity that can be fully restored by addition of 1 mM Ca2+ Homo sapiens
3.4.24.17 Co2+ addition of 0.5 mM Co2+ in the presence of Ca2+ restores activity of the apoenzyme, not by Co2+ alone Homo sapiens
3.4.24.17 Mn2+ addition of 5 mM Mn2+ in the presence of Ca2+ restores activity of the apoenzyme, not by Mn2+ alone Homo sapiens
3.4.24.17 Zinc Zn2+: addition of 0.5 mM Zn2+ in the presence of Ca2+ restores activity of the apoenzyme, not by Zn2+ alone Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.17 additional information
-
human, latent precursor, MW 51000, gel filtration Homo sapiens
3.4.24.17 28000
-
x * 28000, human, form LMW, SDS-PAGE Homo sapiens
3.4.24.17 45000
-
x * 45000, human, form HMW, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.17 additional information Homo sapiens may play a role in the normal turnover of the connective tissue matrix as well as in the joint destruction of chronic synovitis ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.17 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.17 2 active forms: HMW, LMW Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.17 culture medium of rheumatoid synovial cells Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.17 additional information
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.4.24.17 -20°C, 0°C or 4°C, 50 mM Tris-HCl, pH 7.5, 0.4 M NaCl, 10 mM Ca2+, 0.05% Brij 35, 0.02% NaN3, less than 19% loss of activity after 10 weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.17 Collagen type IV + H2O
-
Homo sapiens More 4 major fragments of MW 165000, 145000, 125000 and 110000 ?
3.4.24.17 Fibronectin + H2O
-
Homo sapiens Intact fibronectin subunit MW 22000 + a disulfide-bonded COOH-terminal MW 20000 polypeptide
-
?
3.4.24.17 Gelatin + H2O type 1 Homo sapiens ?
-
?
3.4.24.17 Laminin + H2O
-
Homo sapiens ?
-
?
3.4.24.17 additional information may play a role in the normal turnover of the connective tissue matrix as well as in the joint destruction of chronic synovitis Homo sapiens ?
-
?
3.4.24.17 Procollagen + H2O removal of the NH2-terminal propeptides Homo sapiens ?
-
?
3.4.24.17 Procollagen + H2O chicken type I Homo sapiens ?
-
?
3.4.24.17 Proteoglycan + H2O cartilage Homo sapiens More degradation to 12.0 S fragments by the HMW form and 10.3 S fragments by the KMW enzyme ?

Subunits

EC Number Subunits Comment Organism
3.4.24.17 ? x * 45000, human, form HMW, SDS-PAGE Homo sapiens
3.4.24.17 ? x * 28000, human, form LMW, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.17 80
-
15 min: 35% loss activity of enzyme form HMW, 5% loss of sctivity of enzyme form LMW Homo sapiens
3.4.24.17 100
-
15 min, 65% loss of activity of enzyme form HMW, 25% loss of activity of enzyme form LMW Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.17 7.5 7.8
-
Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.17 6 8.5 about 50% of activity maximum at pH 6.0 and 8.5 Homo sapiens