BRENDA - Enzyme Database show

Purification and properties of prostaglandin D synthetase from rat brain

Shimizu, T.; Yamamoto, S.; Hayaishi, O.; J. Biol. Chem. 254, 5222-5228 (1979)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.2
glutathione
not required
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
5.3.99.2
thiol compounds, including glutathione stabilize
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.2
iodoacetamide
-
Rattus norvegicus
5.3.99.2
NEM
-
Rattus norvegicus
5.3.99.2
PCMB
reversible
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.2
0.008
-
prostaglandin H2
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.2
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.2
80000
-
1 * 80000, SDS-PAGE
Rattus norvegicus
5.3.99.2
85000
-
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.2
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.2
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.2
1.7
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
5.3.99.2
-80░C, stable for at least 1 month
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
prostaglandin G2
-
3100
Rattus norvegicus
15-hydroperoxyprostaglandin D2
-
3100
Rattus norvegicus
-
5.3.99.2
Prostaglandin H2
-
3100
Rattus norvegicus
Prostaglandin D2
-
3100
Rattus norvegicus
-
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.2
monomer
1 * 80000, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.2
8
-
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.2
glutathione
not required
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
5.3.99.2
thiol compounds, including glutathione stabilize
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.2
iodoacetamide
-
Rattus norvegicus
5.3.99.2
NEM
-
Rattus norvegicus
5.3.99.2
PCMB
reversible
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.2
0.008
-
prostaglandin H2
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.2
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.2
80000
-
1 * 80000, SDS-PAGE
Rattus norvegicus
5.3.99.2
85000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.2
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.2
1.7
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
5.3.99.2
-80░C, stable for at least 1 month
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
prostaglandin G2
-
3100
Rattus norvegicus
15-hydroperoxyprostaglandin D2
-
3100
Rattus norvegicus
-
5.3.99.2
Prostaglandin H2
-
3100
Rattus norvegicus
Prostaglandin D2
-
3100
Rattus norvegicus
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.2
monomer
1 * 80000, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.2
8
-
-
Rattus norvegicus