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Literature summary extracted from
- Purification of PGH-PDG isomerase from rat brain (1982), Methods Enzymol., 86, 73-77.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.99.2 | glutathione | not required | Rattus norvegicus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.99.2 | iodoacetamide | - |
Rattus norvegicus | |
| 5.3.99.2 | NEM | - |
Rattus norvegicus | |
| 5.3.99.2 | PCMB | - |
Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.99.2 | 0.008 | - |
prostaglandin H2 | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.3.99.2 | cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.2 | 26000 | - |
x * 26000, SDS-PAGE | Rattus norvegicus |
| 5.3.99.2 | 80000 | 85000 | - |
Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.99.2 | Cavia porcellus | - |
- |
- |
| 5.3.99.2 | Felis catus | - |
low activity | - |
| 5.3.99.2 | Mus musculus | - |
- |
- |
| 5.3.99.2 | Oryctolagus cuniculus | - |
low activity | - |
| 5.3.99.2 | Platyrrhini | - |
- |
- |
| 5.3.99.2 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.99.2 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.3.99.2 | brain | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.2 | 1.68 | - |
- |
Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.3.99.2 | 4°C, 24 h, 50% loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.99.2 | Prostaglandin H2 | - |
Rattus norvegicus | Prostaglandin D2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.99.2 | ? | x * 26000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.2 | 8 | - |
- |
Rattus norvegicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.2 | 5 | - |
4°C, 24 h, 40% loss of activity | Rattus norvegicus |
| 5.3.99.2 | 6 | - |
4°C, 24 h, 5% loss of activity | Rattus norvegicus |
| 5.3.99.2 | 7 | - |
4°C, 24 h, 40% loss of activity | Rattus norvegicus |
| 5.3.99.2 | 8 | - |
4°C, 24 h, 60% loss of activity | Rattus norvegicus |
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