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Literature summary extracted from

  • Jupp, R.A.; Richards, A.D.; Kay, J.; Dunn, B.M.; Wyckoff, J.B.; Samloff, M.; Yamamoto, K.
    Identification of the aspartic proteinases from human erythrocyte membranes and gastric mucosa (slow-moving proteinase) as catalytically equivalent to cathepsin E (1988), Biochem. J., 254, 895-898.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.34 (D)-His-Pro-Phe-His-Leu-PSI(CH2-NH)-Leu-Val-Tyr i.e. H-77, with reduced isostere as replacement of the-CO-NH- of the peptide bond Homo sapiens
3.4.23.34 (D)-His-Pro-Phe-His-Leu-PSI(CH2-NH)-Leu-Val-Tyr i.e. H-77, with reduced isostere as replacement of the-CO-NH- of the peptide bond Rattus norvegicus
3.4.23.34 N-tert-Butoxycarbonyl-His-Pro-Phe-His-4-amino-3-hydroxy-6-methylheptanoic acid-Leu-Phe-NH2
-
 Homo sapiens
3.4.23.34 N-tert-Butoxycarbonyl-His-Pro-Phe-His-4-amino-3-hydroxy-6-methylheptanoic acid-Leu-Phe-NH2
-
 Rattus norvegicus
3.4.23.34 N-tert-Butoxycarbonyl-His-Pro-Phe-His-Leu-PSI(CHOH-CH2)-Val-Ile-His i.e. H-261, with reduced isostere as replacement of the -CO-NH- of the peptide bond Homo sapiens
3.4.23.34 N-tert-Butoxycarbonyl-His-Pro-Phe-His-Leu-PSI(CHOH-CH2)-Val-Ile-His i.e. H-261, with reduced isostere as replacement of the -CO-NH- of the peptide bond Rattus norvegicus
3.4.23.34 pepstatin
-
 Homo sapiens
3.4.23.34 pepstatin
-
 Oryctolagus cuniculus
3.4.23.34 pepstatin
-
 Rattus norvegicus
3.4.23.34 Pro-Thr-Glu-Phe-PSI(CH2-NH)-Nle-Arg-Leu i.e. H-297, with reduced isostere as replacement of the -CO-NH- of the peptide bond Homo sapiens
3.4.23.34 Pro-Thr-Glu-Phe-PSI(CH2-NH)-Nle-Arg-Leu i.e. H-297, with reduced isostere as replacement of the -CO-NH- of the peptide bond Rattus norvegicus
3.4.23.34 Pro-Thr-Glu-Phe-PSI(CH2-NH)-Phe-Arg-Glu i.e. H-256, with reduced isostere as replacement of the -CO-NH- of the peptide bond Homo sapiens
3.4.23.34 Pro-Thr-Glu-Phe-PSI(CH2-NH)-Phe-Arg-Glu i.e. H-256, with reduced isostere as replacement of the -CO-NH- of the peptide bond Rattus norvegicus
3.4.23.34 Protein inhibitor from Ascaris lumbricoides MW 17000 Homo sapiens
3.4.23.34 Protein inhibitor from Ascaris lumbricoides MW 17000 Oryctolagus cuniculus
3.4.23.34 Protein inhibitor from Ascaris lumbricoides MW 17000 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.34 additional information
-
 additional information pH-dependence of kinetic constants Homo sapiens
3.4.23.34 additional information
-
 additional information pH-dependence of kinetic constants Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.34 42000
-
 2 * 42000, about, SDS-PAGE, reducing conditions Homo sapiens
3.4.23.34 42000
-
 2 * 42000, about, SDS-PAGE, reducing conditions Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.34 Homo sapiens
-
-
-
3.4.23.34 Oryctolagus cuniculus
-
-
-
3.4.23.34 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.34 erythrocyte
-
 Homo sapiens
-
3.4.23.34 erythrocyte
-
 Rattus norvegicus
-
3.4.23.34 gastric mucosa
-
 Homo sapiens
-
3.4.23.34 gastric mucosa
-
 Rattus norvegicus
-
3.4.23.34 gastric mucosa
-
 Oryctolagus cuniculus
-
3.4.23.34 spleen
-
 Rattus norvegicus
-
3.4.23.34 stomach
-
 Homo sapiens
-
3.4.23.34 stomach
-
 Rattus norvegicus
-
3.4.23.34 stomach
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.34 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Homo sapiens Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.23.34 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Rattus norvegicus Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.23.34 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Oryctolagus cuniculus Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.23.34 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O synthetic chromogenic peptide Homo sapiens Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.23.34 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O synthetic chromogenic peptide Rattus norvegicus Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.34 dimer
-
 Oryctolagus cuniculus
3.4.23.34 dimer 2 * 42000, about, SDS-PAGE, reducing conditions Homo sapiens
3.4.23.34 dimer 2 * 42000, about, SDS-PAGE, reducing conditions Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.34 37
-
 assay at Homo sapiens
3.4.23.34 37
-
 assay at Rattus norvegicus
3.4.23.34 37
-
 assay at Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.34 additional information
-
 additional information pH-dependence of kinetic constants Homo sapiens
3.4.23.34 additional information
-
 additional information pH-dependence of kinetic constants Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.34 5.8
-
 75% loss of activity of erythrocyte membrane aspartic proteinase from human erythrocytes, inactivation of slow-moving proteinase from human gastric mucosa and cathepsin E from rat spleen, instable above pH 5.8 Homo sapiens
3.4.23.34 5.8
-
 75% loss of activity of erythrocyte membrane aspartic proteinase from human erythrocytes, inactivation of slow-moving proteinase from human gastric mucosa and cathepsin E from rat spleen, instable above pH 5.8 Rattus norvegicus