EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.23.32 | 1,2-epoxy-3-(4-nitrophenoxy)propane | not | Ganoderma lucidum | |
3.4.23.32 | additional information | diazoacetyl-DL-norleucine methyl ester; methyl 2-diazoacetamidohexanoate; not: pepstatin Ac; Streptomyces-pepsin inhibitor (S-PI) | Ganoderma lucidum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.23.32 | 36000 | - |
Ganoderma lucidum, gel filtration | Ganoderma lucidum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.23.32 | Ganoderma lucidum | - |
similar enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.23.32 | - |
Ganoderma lucidum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.23.32 | culture filtrate | - |
Ganoderma lucidum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.23.32 | additional information | - |
- |
Ganoderma lucidum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.23.32 | casein + H2O | 15% of the activity with hemoglobin | Ganoderma lucidum | hydrolyzed casein | - |
? | |
3.4.23.32 | Hemoglobin + H2O | - |
Ganoderma lucidum | Hydrolyzed hemoglobin | - |
? | |
3.4.23.32 | Oxidized insulin B-chain + H2O | splits preferentially the Phe24-Phe25 bond at the rate 50% for total hydrolysis | Ganoderma lucidum | Hydrolyzed insulin B-chain | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.23.32 | monomer | 1 * 36000, Ganoderma lucidum, SDS-PAGE | Ganoderma lucidum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.23.32 | 52 | - |
- |
Ganoderma lucidum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.23.32 | 37 | - |
pH 3.2-5.2, 3 h, stable | Ganoderma lucidum |
3.4.23.32 | 42 | - |
pH 4.0, 30 min, stable below | Ganoderma lucidum |
3.4.23.32 | 58 | - |
pH 4, 30 min, complete loss of activity | Ganoderma lucidum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.23.32 | 2 | - |
casein | Ganoderma lucidum |
3.4.23.32 | 3.2 | - |
hemoglobin | Ganoderma lucidum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.23.32 | 2 | 5 | 2: about 30% of activity maximum, 5: about 50% of activity maximum, hemoglobin | Ganoderma lucidum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.23.32 | 3.2 | 5.2 | 37°C, 3 h, stable | Ganoderma lucidum |
3.4.23.32 | 4 | - |
58°C | Ganoderma lucidum |
3.4.23.32 | 4 | - |
stable below 42°C, 30 min | Ganoderma lucidum |