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Literature summary extracted from
- Purification and properties of proteinase A from yeast (1980), J. Biol. Chem., 255, 12087-12093.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.23.25 | Quite stable in concentrated solutions of urea, retains full activity after 1 h at 4°C in 6 M urea at pH 6.0, significant loss of activity at pH 5.0 and 7.0 | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.25 | 1,2-epoxy-3-(4-nitrophenoxy)propane | - |
Saccharomyces cerevisiae |  |
| 3.4.23.25 | Diazoacetyl-DL-norleucine methyl ester | in presence of copper ions | Saccharomyces cerevisiae | |
| 3.4.23.25 | pepstatin | - |
Saccharomyces cerevisiae | |
| 3.4.23.25 | Specific proteinase inhibitor from yeast | - |
Saccharomyces cerevisiae |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.25 | 41400 | - |
sedimentation-diffusion equilibrium ultracentrifugation | Saccharomyces cerevisiae |
| 3.4.23.25 | 41700 | - |
1 * 41700, Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.25 | Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.23.25 | glycoprotein | contains 7.5% w/w mannose and 1% of glucosamine and galactosamine | Saccharomyces cerevisiae |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.25 | - |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.25 | additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.25 | azocasein + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.23.25 | casein + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.23.25 | Hemoglobin + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.23.25 | monomer | 1 * 41700, Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.25 | 25 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.25 | 25 | - |
pH 4-6: stable, pH 3: 80% loss of activity | Saccharomyces cerevisiae |
| 3.4.23.25 | 45 | - |
pH 5.5, 2 h, stable below | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.25 | 3 | - |
hemoglobin | Saccharomyces cerevisiae |
| 3.4.23.25 | 6 | - |
casein, azocasein | Saccharomyces cerevisiae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.25 | 3 | - |
25°C, 48 h, 80% loss of activity | Saccharomyces cerevisiae |
| 3.4.23.25 | 4 | 6 | 25°C, stable | Saccharomyces cerevisiae |
| 3.4.23.25 | 5.5 | - |
2 h, stable below 45°C | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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