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Literature summary extracted from
- Properties of a purified proteinase from the yeast Candida albicans (1981), Biochim. Biophys. Acta, 659, 99-113.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.24 | nonionic detergents | Triton X-100, Tween-80, Nonidet P-40, slight improvement of activity | Candida albicans |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.23.24 | Stable in presence of non-ionic detergents | Candida albicans |
| 3.4.23.24 | Stable to freeze-drying | Candida albicans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.24 | 1,2-epoxy-3-(4-nitrophenoxy)propane | not | Candida albicans |  |
| 3.4.23.24 | additional information | methyl 2-diazoacetamidohexanoate | Candida albicans | |
| 3.4.23.24 | pepstatin | - |
Candida albicans | |
| 3.4.23.24 | SDS | tolerated at 0.01% w/v, decrease of activity at 0.1% w/v, 1% completely destroys activity within a few min | Candida albicans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.24 | 0.07 | - |
albumin | - |
Candida albicans |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.24 | 45000 | - |
Candida albicans, nondenaturing PAGE | Candida albicans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.24 | Candida albicans | - |
- |
- |
| 3.4.23.24 | Candida albicans CBS-2730 | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.23.24 | glycoprotein | - |
Candida albicans |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.24 | - |
Candida albicans |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.23.24 | culture supernatant | - |
Candida albicans | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.24 | additional information | - |
- |
Candida albicans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.24 | Albumin + H2O | - |
Candida albicans | ? | - |
? | |
| 3.4.23.24 | Albumin + H2O | - |
Candida albicans CBS-2730 | ? | - |
? | |
| 3.4.23.24 | additional information | trypsinogen kinase activity | Candida albicans | ? | - |
? | |
| 3.4.23.24 | additional information | not: horse ferritin | Candida albicans | ? | - |
? | |
| 3.4.23.24 | additional information | clots milk at pH 5.5 | Candida albicans | ? | - |
? | |
| 3.4.23.24 | additional information | trypsinogen kinase activity | Candida albicans CBS-2730 | ? | - |
? | |
| 3.4.23.24 | additional information | not: horse ferritin | Candida albicans CBS-2730 | ? | - |
? | |
| 3.4.23.24 | additional information | clots milk at pH 5.5 | Candida albicans CBS-2730 | ? | - |
? | |
| 3.4.23.24 | Myeloma proteins of the type A1 and A2 + H2O | - |
Candida albicans | ? | - |
? | |
| 3.4.23.24 | Myeloma proteins of the type A1 and A2 + H2O | - |
Candida albicans CBS-2730 | ? | - |
? | |
| 3.4.23.24 | Proteinase inhibitors of human serum + H2O | - |
Candida albicans | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.23.24 | monomer | 1 * 45000, Candida albicans, SDS-PAGE | Candida albicans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.24 | 37 | - |
10 min, 85% loss of activity at pH 7.5, 5% loss of activity at pH 6.6 and 7.0 | Candida albicans |
| 3.4.23.24 | 45 | - |
10 min, complete loss of activity | Candida albicans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.24 | 2.5 | 3.9 | hemoglobin | Candida albicans |
| 3.4.23.24 | 3.7 | - |
bovine serum albumin | Candida albicans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.24 | 8.4 | - |
above, alkaline denaturation accompanied by dimerization | Candida albicans |
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