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Literature summary extracted from

  • Tsujita, Y.; Endo, A.
    Presence and partial characterization of internal acid protease of Aspergillus oryzae (1978), Appl. Environ. Microbiol., 36, 237-242.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.23.18 Triton X-100 not Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.18 additional information soybean trypsin inhibitor; Triton X-100 Aspergillus oryzae
3.4.23.18 SDS
-
Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.18 cytoplasm 2 forms: extracellular and cytoplasmic, the cytoplasmic isozymes are immunologically related to extracellular isozyme A2 Aspergillus oryzae 5737
-
3.4.23.18 extracellular 2 forms: extracellular and cytoplasmic Aspergillus oryzae
-
-
3.4.23.18 soluble
-
Aspergillus oryzae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.18 42000
-
Aspergillus oryzae, enzyme form F2, gel filtration Aspergillus oryzae
3.4.23.18 60000
-
Aspergillus oryzae, enzyme form F1, gel filtration Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.18 Aspergillus oryzae
-
-
-
3.4.23.18 Aspergillus oryzae 365-U-64-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.18 cytoplasmic enzyme: partial Aspergillus oryzae
3.4.23.18 several isoforms (cytoplasmic F1 and F2) Aspergillus oryzae
3.4.23.18 several isoforms (extracellular A1 and A2) Aspergillus oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.18 commercial preparation extracellular enzyme Aspergillus oryzae
-
3.4.23.18 commercial preparation Taka-diastase, crude Aspergillus oryzae powder preparation Aspergillus oryzae
-
3.4.23.18 mycelium cytoplasmic enzyme Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.18 additional information
-
-
Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.18 Cytochrome c + H2O
-
Aspergillus oryzae ?
-
?
3.4.23.18 Cytochrome c + H2O
-
Aspergillus oryzae 365-U-64-1 ?
-
?
3.4.23.18 Hemoglobin + H2O urea-denatured hemoglobin Aspergillus oryzae ?
-
?
3.4.23.18 Hemoglobin + H2O urea-denatured hemoglobin Aspergillus oryzae 365-U-64-1 ?
-
?
3.4.23.18 Trypsinogen + H2O bovine (pancreatic) Aspergillus oryzae ?
-
?
3.4.23.18 Trypsinogen + H2O bovine (trypsinogen) Aspergillus oryzae ?
-
?
3.4.23.18 Trypsinogen + H2O bovine (pancreatic) Aspergillus oryzae 365-U-64-1 ?
-
?
3.4.23.18 Trypsinogen + H2O bovine (trypsinogen) Aspergillus oryzae 365-U-64-1 ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.23.18 55
-
10 min, in 50 mM sodium acetate buffer, pH 5 Aspergillus oryzae
3.4.23.18 55
-
isozyme F1 retains 70%, F2 85% of original activity Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.18 3
-
cytochrome c Aspergillus oryzae
3.4.23.18 4.2
-
urea-denatured hemoglobin Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.18 2.3 4.3 about half-maximal activity at pH 2.3 and 4.3, cytochrome c Aspergillus oryzae
3.4.23.18 3.5 6.5 about half-maximal activity at pH 3.5 and 6.5, urea-denatured hemoglobin Aspergillus oryzae