EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.23.18 | Triton X-100 | not | Aspergillus oryzae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.23.18 | additional information | soybean trypsin inhibitor; Triton X-100 | Aspergillus oryzae | |
3.4.23.18 | SDS | - |
Aspergillus oryzae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.23.18 | cytoplasm | 2 forms: extracellular and cytoplasmic, the cytoplasmic isozymes are immunologically related to extracellular isozyme A2 | Aspergillus oryzae | 5737 | - |
3.4.23.18 | extracellular | 2 forms: extracellular and cytoplasmic | Aspergillus oryzae | - |
- |
3.4.23.18 | soluble | - |
Aspergillus oryzae | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.23.18 | 42000 | - |
Aspergillus oryzae, enzyme form F2, gel filtration | Aspergillus oryzae |
3.4.23.18 | 60000 | - |
Aspergillus oryzae, enzyme form F1, gel filtration | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.23.18 | Aspergillus oryzae | - |
- |
- |
3.4.23.18 | Aspergillus oryzae 365-U-64-1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.23.18 | cytoplasmic enzyme: partial | Aspergillus oryzae |
3.4.23.18 | several isoforms (cytoplasmic F1 and F2) | Aspergillus oryzae |
3.4.23.18 | several isoforms (extracellular A1 and A2) | Aspergillus oryzae |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.23.18 | commercial preparation | extracellular enzyme | Aspergillus oryzae | - |
3.4.23.18 | commercial preparation | Taka-diastase, crude Aspergillus oryzae powder preparation | Aspergillus oryzae | - |
3.4.23.18 | mycelium | cytoplasmic enzyme | Aspergillus oryzae | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.23.18 | additional information | - |
- |
Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.23.18 | Cytochrome c + H2O | - |
Aspergillus oryzae | ? | - |
? | |
3.4.23.18 | Cytochrome c + H2O | - |
Aspergillus oryzae 365-U-64-1 | ? | - |
? | |
3.4.23.18 | Hemoglobin + H2O | urea-denatured hemoglobin | Aspergillus oryzae | ? | - |
? | |
3.4.23.18 | Hemoglobin + H2O | urea-denatured hemoglobin | Aspergillus oryzae 365-U-64-1 | ? | - |
? | |
3.4.23.18 | Trypsinogen + H2O | bovine (pancreatic) | Aspergillus oryzae | ? | - |
? | |
3.4.23.18 | Trypsinogen + H2O | bovine (trypsinogen) | Aspergillus oryzae | ? | - |
? | |
3.4.23.18 | Trypsinogen + H2O | bovine (pancreatic) | Aspergillus oryzae 365-U-64-1 | ? | - |
? | |
3.4.23.18 | Trypsinogen + H2O | bovine (trypsinogen) | Aspergillus oryzae 365-U-64-1 | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.23.18 | 55 | - |
10 min, in 50 mM sodium acetate buffer, pH 5 | Aspergillus oryzae |
3.4.23.18 | 55 | - |
isozyme F1 retains 70%, F2 85% of original activity | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.23.18 | 3 | - |
cytochrome c | Aspergillus oryzae |
3.4.23.18 | 4.2 | - |
urea-denatured hemoglobin | Aspergillus oryzae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.23.18 | 2.3 | 4.3 | about half-maximal activity at pH 2.3 and 4.3, cytochrome c | Aspergillus oryzae |
3.4.23.18 | 3.5 | 6.5 | about half-maximal activity at pH 3.5 and 6.5, urea-denatured hemoglobin | Aspergillus oryzae |