Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Murachi, T.
    Bromelain enzymes (1976), Methods Enzymol., 45, 475-485.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.32 alpha2-Macroglobulin
-
 Ananas comosus
3.4.22.32 Human plasma
-
 Ananas comosus
3.4.22.32 additional information alpha1-antitrypsin Ananas comosus
3.4.22.32 Rabbit anti-stem bromelain antibodies
-
 Ananas comosus
3.4.22.33 Mercurials cysteine restores activity; mercaptoethanol restores activity Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 0.53
-
 N-Benzyloxycarbonyl-L-Phe-L-Ser methyl ester
-
 Ananas comosus
3.4.22.33 4
-
 Nalpha-benzoyl-Arg amide
-
 Ananas comosus
3.4.22.33 43
-
 N-benzoyl-L-Arg ethyl ester
-
 Ananas comosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.32 33000
-
 Ananas comosus, determination from sedimentation and diffusion coefficient Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
-
-
3.4.22.33 Ananas comosus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.4.22.32 photosensitized oxidation of stem bromelain in the presence of methylene blue results in partial loss of the enzymatic activity even if the essential sulfhydryl group is protected against oxidation Ananas comosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.32
-
 Ananas comosus
3.4.22.33
-
 Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem
-
 Ananas comosus
-
3.4.22.33 fruit
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 Benzoyl-L-Arg amide + H2O
-
 Ananas comosus Benzoyl-L-Arg + NH3
-
 ?
3.4.22.32 Hemoglobin + H2O denatured Ananas comosus Hydrolyzed hemoglobin
-
 ?
3.4.22.32 N-Benzyloxycarbonyl-L-Phe-L-Ser methyl ester + H2O
-
 Ananas comosus ?
-
 ?
3.4.22.33 angiotensin II + H2O cleavage between Tyr4-Ile5 Ananas comosus hydrolyzed angiotensin II cleavage between Tyr4-Ile5 ?
3.4.22.33 bradykinin + H2O cleavage between Gly4-Phe5 and Phe5-Ser6 Ananas comosus fragments of bradykinin cleavage between Gly4-Phe5 and Phe5-Ser6 ?
3.4.22.33 additional information enzyme catalyzes synthesis of acylamino acid anilides Ananas comosus ?
-
 ?
3.4.22.33 N-Benzoyl-L-Arg ethyl ester + H2O
-
 Ananas comosus N-Benzoyl-L-Arg + ethanol
-
 ?
3.4.22.33 Nalpha-Benzoyl-Arg amide + H2O
-
 Ananas comosus Nalpha-Benzoyl-Arg + NH3
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.32 3.4
-
 N-Benzyloxycarbonyl-L-Phe-L-Ser methyl ester
-
 Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.32 5
-
 denatured hemoglobin, enzyme form F4 Ananas comosus
3.4.22.32 5 8 wide plateau, Nalpha-benzoyl-L-Arg amide Ananas comosus
3.4.22.33 8.3
-
 casein Ananas comosus