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Literature summary extracted from

  • Bruinenberg, P.G.; de Vos, W.M.; Siezen, R.J.
    Prevention of C-terminal autoprocessing of Lactococcus lactis SK11 cell-envelope proteinase by engineering of an essential surface loop (1994), Biochem. J., 302, 957-963.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.96
-
 Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.96 additional information in strain SK11 the mutagenesis of the loop 205-219 region of the catalytic domain results in inactivation, mostly due to inhibiton of autoprocessing of proenzyme Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.96 cell envelope
-
 Lactococcus lactis 30313
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.96 145000
-
 strain SK11, x * 145000, SDS-PAGE Lactococcus lactis
3.4.21.96 200000
-
 strain SK11, x * 200000, inactive, unprocessed precursor of an active-site mutant Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.96 Usp 45 + H2O Lactococcus lactis a secreted 60 kDa protein of Lactococcus lactis ?
-
 ?
3.4.21.96 Usp 45 + H2O Lactococcus lactis SK11 a secreted 60 kDa protein of Lactococcus lactis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.96 Lactococcus lactis
-
 SK11
-
3.4.21.96 Lactococcus lactis SK11
-
 SK11
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.96 alphas1-Casein + H2O
-
 Lactococcus lactis Fragments of alphas1-casein
-
 ?
3.4.21.96 alphas1-Casein + H2O
-
 Lactococcus lactis SK11 Fragments of alphas1-casein
-
 ?
3.4.21.96 alphas1-Casein fragment 1-23 + H2O
-
 Lactococcus lactis Fragments of alphas1-casein fragment 1-23
-
 ?
3.4.21.96 alphas1-Casein fragment 1-23 + H2O
-
 Lactococcus lactis SK11 Fragments of alphas1-casein fragment 1-23
-
 ?
3.4.21.96 beta-Casein + H2O
-
 Lactococcus lactis Fragments of beta-casein
-
 ?
3.4.21.96 beta-Casein + H2O
-
 Lactococcus lactis SK11 Fragments of beta-casein
-
 ?
3.4.21.96 Usp 45 + H2O a secreted 60 kDa protein of Lactococcus lactis Lactococcus lactis ?
-
 ?
3.4.21.96 Usp 45 + H2O a secreted 60 kDa protein of Lactococcus lactis Lactococcus lactis SK11 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.96 ? strain SK11, x * 145000, SDS-PAGE Lactococcus lactis
3.4.21.96 ? strain SK11, x * 200000, inactive, unprocessed precursor of an active-site mutant Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
3.4.21.96 Lactococcal proteinase PI
-
 Lactococcus lactis