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Literature summary extracted from
- Further studies on lipopolysaccharide-sensitive serine protease zymogen (factor C): its isolation from Limulus polyphemus hemocytes and identification as an intracellular zymogen activated by alpha-chymotrypsin, not by trypsin (1991), J. Biochem., 109, 150-157.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.84 | additional information | the zymogen factor C shows autocatalytic activation in presence of bacterial lipopolysaccharide | Limulus polyphemus | |
| 3.4.21.84 | additional information | the zymogen factor C shows autocatalytic activation in presence of bacterial lipopolysaccharide | Tachypleus tridentatus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.84 | D-Phe-Pro-Arg chloromethyl ketone | - |
Limulus polyphemus |  |
| 3.4.21.84 | D-Phe-Pro-Arg chloromethyl ketone | - |
Tachypleus tridentatus | |
| 3.4.21.84 | DFP | - |
Limulus polyphemus | |
| 3.4.21.84 | DFP | - |
Tachypleus tridentatus | |
| 3.4.21.84 | leupeptin | weak | Limulus polyphemus | |
| 3.4.21.84 | leupeptin | weak | Tachypleus tridentatus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.84 | additional information | - |
additional information | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 0.026 | - |
tert-butoxycarbonyl-Asp(O-benzyl)-Pro-Arg-7-amido-4-methylcoumarin | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 0.039 | - |
Tert-Butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 0.045 | - |
tert-butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 0.054 | - |
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 0.066 | - |
Tert-Butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 0.088 | - |
Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 0.114 | - |
Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 0.19 | - |
tert-butoxycarbonyl-Ala-Pro-Arg-7-amido-4-methylcoumarin | - |
Tachypleus tridentatus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.84 | intracellular | - |
Limulus polyphemus | 5622 | - |
| 3.4.21.84 | intracellular | - |
Tachypleus tridentatus | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.84 | 43000 | - |
x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol | Limulus polyphemus |
| 3.4.21.84 | 80000 | - |
x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol | Limulus polyphemus |
| 3.4.21.84 | 123000 | - |
x * 123000, Limulus polyphemus, SDS-PAGE under nonreducing conditions | Limulus polyphemus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.84 | additional information | Limulus polyphemus | factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus) | ? | - |
? | |
| 3.4.21.84 | additional information | Tachypleus tridentatus | factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus) | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.84 | Limulus polyphemus | - |
- |
- |
| 3.4.21.84 | Tachypleus tridentatus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.84 | - |
Limulus polyphemus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.84 | hemocyte | - |
Limulus polyphemus | - |
| 3.4.21.84 | hemocyte | - |
Tachypleus tridentatus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.84 | additional information | alpha-thrombin like specificity towards synthetic tripeptide substrates | Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | additional information | alpha-thrombin like specificity towards synthetic tripeptide substrates | Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | additional information | factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus) | Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | additional information | factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus) | Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | N-tert-Butoxycarbonyl-Asp(O-benzyl)-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | N-tert-Butoxycarbonyl-Asp(O-benzyl)-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Ala-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Tachypleus tridentatus | tert-butoxycarbonyl-Ala-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Asp(O-benzyl)-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Tachypleus tridentatus | tert-butoxycarbonyl-Asp(O-benzyl)-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Glu(O-benzyl)-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Glu(O-benzyl)-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Gly-Ala-Arg-4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Gly-Ala-Arg-4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Gly-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Gly-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Ile-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | tert-butoxycarbonyl-Ile-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg-4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg-4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Lys-Gly-Arg 4-methylcoumaryl 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Lys-Gly-Arg 4-methylcoumaryl 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Ser(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Ser(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Val-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Val-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| 3.4.21.84 | Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.84 | ? | x * 123000, Limulus polyphemus, SDS-PAGE under nonreducing conditions | Limulus polyphemus |
| 3.4.21.84 | ? | x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol | Limulus polyphemus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.84 | additional information | - |
additional information | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 10 | - |
tert-butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 18 | - |
Tert-Butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 27 | - |
Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 34 | - |
Tert-Butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 48 | - |
Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 72 | - |
tert-butoxycarbonyl-Ala-Pro-Arg-7-amido-4-methylcoumarin | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 75 | - |
tert-butoxycarbonyl-Asp(O-benzyl)-Pro-Arg-7-amido-4-methylcoumarin | - |
Tachypleus tridentatus | |
| 3.4.21.84 | 391 | - |
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide | - |
Tachypleus tridentatus | |
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