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Literature summary extracted from
- Oligopeptidase B: protease II from Escherichia coli (1994), Methods Enzymol., 244, 201-215.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.83 | - |
Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.83 | antipain | - |
Escherichia coli |  |
| 3.4.21.83 | DFP | - |
Escherichia coli | |
| 3.4.21.83 | additional information | several natural trypsin inhibitors | Escherichia coli | |
| 3.4.21.83 | tosyl-Leu chloromethyl ketone | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.83 | additional information | - |
additional information | - |
Escherichia coli | |
| 3.4.21.83 | 0.25 | - |
benzoyl-Arg 2-naphthylamide | - |
Escherichia coli | |
| 3.4.21.83 | 0.48 | - |
benzoyl-Arg ethyl ester | - |
Escherichia coli | |
| 3.4.21.83 | 0.5 | - |
benzoyl-Arg 4-nitroanilide | - |
Escherichia coli | |
| 3.4.21.83 | 0.92 | - |
Benzoyl-Lys 2-naphthylamide | - |
Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.83 | Escherichia coli | - |
Escherichia coli JM83 harboring pPR0II-12 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.83 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.83 | additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.83 | Benzoyl-Lys 2-naphthylamide + H2O | - |
Escherichia coli | Benzoyl-Lys + 2-naphthylamine | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.83 | 45 | - |
30 min, pH 8.0, 50% loss of activity | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.83 | additional information | - |
pI: 5.2 | Escherichia coli |
| 3.4.21.83 | 8 | - |
- |
Escherichia coli |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.83 | 5.5 | 9.5 | stable | Escherichia coli |
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