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Literature summary extracted from

  • Kanatani, A.; Masuda, T.; Shimoda, T.; Misoka, F.; Lin, X.S.; Yoshimoto, T.; Tsuru, D.
    Protease II from Escherichia coli: sequencing and expression of the enzyme gene and characterization of the expressed enzyme (1991), J. Biochem., 110, 315-320.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.83 Escherichia coli HB101 gene cloned and expressed in Escherichia coli JM83 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 antipain
-
 Escherichia coli
3.4.21.83 DFP
-
 Escherichia coli
3.4.21.83 leupeptin weak Escherichia coli
3.4.21.83 tosyl-Leu chloromethyl ketone
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.83 0.25
-
 benzoyl-Arg 2-naphthylamide
-
 Escherichia coli
3.4.21.83 0.92
-
 Benzoyl-Lys 2-naphthylamide
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.83 additional information
-
 Ser532 is the reactive serine residue Escherichia coli
3.4.21.83 80000
-
 1 * 80000, SDS-PAGE without mercaptoethanol Escherichia coli
3.4.21.83 81000
-
 E. coli, gel filtration Escherichia coli
3.4.21.83 81860
-
 E. coli, calculation from nucleotide sequence Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Escherichia coli
-
 HB101 gene cloned and expressed in JM83
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.83 HB101 gene cloned and expressed in JM83 Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.83 cell culture
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.83 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 Benzoyl-Lys 2-naphthylamide + H2O
-
 Escherichia coli Benzoyl-Lys + 2-naphthylamine
-
 ?
3.4.21.83 Insulin B-chain + H2O cleavage at the carboxyl sides of Arg and Lys residues Escherichia coli ?
-
 ?
3.4.21.83 N-Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O weak Escherichia coli N-Benzyloxycarbonyl-Gly + 4-nitrophenol
-
 ?
3.4.21.83 Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O
-
 Escherichia coli Nalpha-benzoyl-L-Arg + 2-naphthylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.83 monomer 1 * 80000, SDS-PAGE without mercaptoethanol Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.83 37
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.83 45
-
 30 min, pH 8.0, 50% loss of activity Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.83 0.024
-
 benzoyl-Arg 2-naphthylamide
-
 Escherichia coli
3.4.21.83 4.4
-
 Benzoyl-Lys 2-naphthylamide
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.83 additional information
-
 pI: 5.2 Escherichia coli
3.4.21.83 8
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.83 5.5 9.5 stable Escherichia coli