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Literature summary extracted from

  • Mallory, P.A.; Travis, J.
    Human pancreatic enzymes: purification and characterization of a nonelastolytic enzyme, protease E. resembling elastase (1975), Biochemistry, 14, 722-730.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.36 dilute solutions, stable Homo sapiens
3.4.21.70 Rapid autolysis at protein concentrations above 0.4 mg/ml, stable below Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.70 Acetyl-Ala-Ala-Ala-AlaCH2Cl
-
 Homo sapiens
3.4.21.70 Acetyl-Ala-Ala-AlaCH2Cl
-
 Homo sapiens
3.4.21.70 Acetyl-Ala-Ala-Phe-AlaCH2Cl
-
 Homo sapiens
3.4.21.70 Acetyl-Ala-Ala-Pro-AlaCH2Cl
-
 Homo sapiens
3.4.21.70 Carboxybenzoyl-Gly-Leu-AlaCH2Cl
-
 Homo sapiens
3.4.21.70 Human serum alpha1-antitrypsin no inhibition by other naturally occuring inhibitors tested Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.70 0.166
-
 tert-butyloxycarbonyl-L-alanine 4-nitrophenyl ester
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.36 30000
-
 gel filtration Homo sapiens
3.4.21.36 30790
-
 amino acid analysis Homo sapiens
3.4.21.70 30000
-
 human, gel filtration Homo sapiens
3.4.21.70 30500
-
 1 * 30500, human, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.36 Homo sapiens
-
-
-
3.4.21.70 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.36
-
 Homo sapiens
3.4.21.70
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.36 pancreas
-
 Homo sapiens
-
3.4.21.70 pancreas
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.36 additional information
-
-
 Homo sapiens
3.4.21.70 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.4.21.70 24°C, dilute solution of protein concentration of less than 0.4 mg/ml, pH 4.0-8.0, stable for 3 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.70 Carboxybenzoyl-L-alanine 4-nitrophenyl ester + H2O
-
 Homo sapiens Carboxybenzoyl-L-alanine + 4-nitrophenol
-
 ?
3.4.21.70 casein + H2O
-
 Homo sapiens hydrolyzed casein
-
 ?
3.4.21.70 Hemoglobin + H2O
-
 Homo sapiens Hydrolyzed hemoglobin
-
 ?
3.4.21.70 additional information no activity with: benzoyl-L-arginine ethyl ester, tosyl-L-arginine methyl ester Homo sapiens ?
-
 ?
3.4.21.70 additional information no elastolytic activity Homo sapiens ?
-
 ?
3.4.21.70 Tert-Butyloxycarbonyl-L-alanine 4-nitrophenyl ester + H2O
-
 Homo sapiens Tert-Butyloxycarbonyl-L-alanine + 4-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.70 monomer 1 * 30500, human, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.70 7.7 9.5 casein Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.36 4 8
-
 Homo sapiens
3.4.21.70 4 8 24°C, dilute solution of protein concentration of less than 0.4 mg/ml, stable for 3 months, unstable below pH 4.0 Homo sapiens