BRENDA - Enzyme Database

Human pancreatic enzymes: purification and characterization of a nonelastolytic enzyme, protease E. resembling elastase

Mallory, P.A.; Travis, J.; Biochemistry 14, 722-730 (1975)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.4.21.36
dilute solutions, stable
Homo sapiens
3.4.21.70
Rapid autolysis at protein concentrations above 0.4 mg/ml, stable below
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.21.70
Acetyl-Ala-Ala-Ala-AlaCH2Cl
-
Homo sapiens
3.4.21.70
Acetyl-Ala-Ala-AlaCH2Cl
-
Homo sapiens
3.4.21.70
Acetyl-Ala-Ala-Phe-AlaCH2Cl
-
Homo sapiens
3.4.21.70
Acetyl-Ala-Ala-Pro-AlaCH2Cl
-
Homo sapiens
3.4.21.70
Carboxybenzoyl-Gly-Leu-AlaCH2Cl
-
Homo sapiens
3.4.21.70
Human serum alpha1-antitrypsin
no inhibition by other naturally occuring inhibitors tested
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.21.70
0.166
-
tert-butyloxycarbonyl-L-alanine 4-nitrophenyl ester
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.21.36
30000
-
gel filtration
Homo sapiens
3.4.21.36
30790
-
amino acid analysis
Homo sapiens
3.4.21.70
30000
-
human, gel filtration
Homo sapiens
3.4.21.70
30500
-
1 * 30500, human, SDS-PAGE
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.21.36
Homo sapiens
-
-
-
3.4.21.70
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.4.21.36
-
Homo sapiens
3.4.21.70
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.21.36
pancreas
-
Homo sapiens
-
3.4.21.70
pancreas
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.21.36
additional information
-
-
Homo sapiens
3.4.21.70
additional information
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
3.4.21.70
24°C, dilute solution of protein concentration of less than 0.4 mg/ml, pH 4.0-8.0, stable for 3 months
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.21.70
Carboxybenzoyl-L-alanine 4-nitrophenyl ester + H2O
-
29635
Homo sapiens
Carboxybenzoyl-L-alanine + 4-nitrophenol
-
-
-
-
3.4.21.70
casein + H2O
-
29635
Homo sapiens
hydrolyzed casein
-
29635
Homo sapiens
-
3.4.21.70
Hemoglobin + H2O
-
29635
Homo sapiens
Hydrolyzed hemoglobin
-
29635
Homo sapiens
-
3.4.21.70
additional information
no activity with: benzoyl-L-arginine ethyl ester, tosyl-L-arginine methyl ester
29635
Homo sapiens
?
-
-
-
-
3.4.21.70
additional information
no elastolytic activity
29635
Homo sapiens
?
-
-
-
-
3.4.21.70
Tert-Butyloxycarbonyl-L-alanine 4-nitrophenyl ester + H2O
-
29635
Homo sapiens
Tert-Butyloxycarbonyl-L-alanine + 4-nitrophenol
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.4.21.70
monomer
1 * 30500, human, SDS-PAGE
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.21.70
7.7
9.5
casein
Homo sapiens
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.4.21.36
4
8
-
Homo sapiens
3.4.21.70
4
8
24°C, dilute solution of protein concentration of less than 0.4 mg/ml, stable for 3 months, unstable below pH 4.0
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
3.4.21.36
dilute solutions, stable
Homo sapiens
3.4.21.70
Rapid autolysis at protein concentrations above 0.4 mg/ml, stable below
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.21.70
Acetyl-Ala-Ala-Ala-AlaCH2Cl
-
Homo sapiens
3.4.21.70
Acetyl-Ala-Ala-AlaCH2Cl
-
Homo sapiens
3.4.21.70
Acetyl-Ala-Ala-Phe-AlaCH2Cl
-
Homo sapiens
3.4.21.70
Acetyl-Ala-Ala-Pro-AlaCH2Cl
-
Homo sapiens
3.4.21.70
Carboxybenzoyl-Gly-Leu-AlaCH2Cl
-
Homo sapiens
3.4.21.70
Human serum alpha1-antitrypsin
no inhibition by other naturally occuring inhibitors tested
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.21.70
0.166
-
tert-butyloxycarbonyl-L-alanine 4-nitrophenyl ester
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.21.36
30000
-
gel filtration
Homo sapiens
3.4.21.36
30790
-
amino acid analysis
Homo sapiens
3.4.21.70
30000
-
human, gel filtration
Homo sapiens
3.4.21.70
30500
-
1 * 30500, human, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.21.36
-
Homo sapiens
3.4.21.70
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.21.36
pancreas
-
Homo sapiens
-
3.4.21.70
pancreas
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.21.36
additional information
-
-
Homo sapiens
3.4.21.70
additional information
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.4.21.70
24°C, dilute solution of protein concentration of less than 0.4 mg/ml, pH 4.0-8.0, stable for 3 months
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.21.70
Carboxybenzoyl-L-alanine 4-nitrophenyl ester + H2O
-
29635
Homo sapiens
Carboxybenzoyl-L-alanine + 4-nitrophenol
-
-
-
-
3.4.21.70
casein + H2O
-
29635
Homo sapiens
hydrolyzed casein
-
29635
Homo sapiens
-
3.4.21.70
Hemoglobin + H2O
-
29635
Homo sapiens
Hydrolyzed hemoglobin
-
29635
Homo sapiens
-
3.4.21.70
additional information
no activity with: benzoyl-L-arginine ethyl ester, tosyl-L-arginine methyl ester
29635
Homo sapiens
?
-
-
-
-
3.4.21.70
additional information
no elastolytic activity
29635
Homo sapiens
?
-
-
-
-
3.4.21.70
Tert-Butyloxycarbonyl-L-alanine 4-nitrophenyl ester + H2O
-
29635
Homo sapiens
Tert-Butyloxycarbonyl-L-alanine + 4-nitrophenol
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.21.70
monomer
1 * 30500, human, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.21.70
7.7
9.5
casein
Homo sapiens
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.4.21.36
4
8
-
Homo sapiens
3.4.21.70
4
8
24°C, dilute solution of protein concentration of less than 0.4 mg/ml, stable for 3 months, unstable below pH 4.0
Homo sapiens