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Literature summary extracted from

  • Binstock, J.F.; Schulz, H.
    Fatty acid oxidation complex from Escherichia coli (1981), Methods Enzymol., 71, 403-411.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.16 inactivation at Tris-HCl concentration Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.35 0.066
-
 acetoacetyl-CoA
-
 Escherichia coli
2.3.1.16 additional information
-
 additional information
-
 Escherichia coli
2.3.1.16 0.031
-
 acetoacetyl-CoA
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.35 270000
-
 gel filtration Escherichia coli
2.3.1.16 270000
-
 multienzyme complex: enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA epimerase and DELTA3-cis-DELTA-trans-enoyl-CoA isomerase, PAGE Escherichia coli
5.1.2.3 42000
-
 multienzyme complex which exhibits activities of EC 4.2.1.17, EC 2.3.1.16, EC 5.1.2.3, and EC 5.3.3.3, 2 * 42000 + 2 * 78000, SDS-PAGE Escherichia coli
5.1.2.3 78000
-
 multienzyme complex which exhibits activities of EC 4.2.1.17, EC 2.3.1.16, EC 5.1.2.3, and EC 5.3.3.3, 2 * 42000 + 2 * 78000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ Escherichia coli
-
 acetoacetyl-CoA + NADH
-
 r
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ Escherichia coli B / ATCC 11303
-
 acetoacetyl-CoA + NADH
-
 r
2.3.1.16 CoA + 3-oxoacyl-CoA Escherichia coli
-
 acyl-CoA + acetyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxoacyl-CoA Escherichia coli B / ATCC 11303
-
 acyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Escherichia coli
-
-
-
2.3.1.16 Escherichia coli
-
-
-
5.1.2.3 Escherichia coli
-
 B
-
5.1.2.3 Escherichia coli B / ATCC 11303
-
 B
-
5.3.3.3 Escherichia coli
-
-
-
5.3.3.3 Escherichia coli B / ATCC 11303
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.1.2.3 lipoprotein the multienzyme complex which exhibits activities of EC 4.2.1.17, EC 2.3.1.16, EC 5.1.2.3, and EC 5.3.3.3, contains phospholipid Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.35 60°C for 10 min, phosphocellulose, Sephacryl S200 Escherichia coli
2.3.1.16 multienzyme complex contains enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA epimerase and DELTA3-cis-DELTA-trans-enoyl-CoA isomerase Escherichia coli
5.1.2.3 multienzyme complex which exhibits activities of EC 4.2.1.17, EC 2.3.1.16, EC 5.1.2.3, EC 1.1.1.35, and EC 5.3.3.3 Escherichia coli
5.3.3.3 fatty acid oxidation complex consisting of EC 4.2.1.17, EC 1.1.1.35, EC 2.3.1.16, EC 5.1.2.3, EC 5.3.3.3 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.16 acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA multienzyme complex contains enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA epimerase : EC 5.1.2.3 and DELTA3-cis-DELTA-trans-enoyl-CoA isomerase: EC 5.3.3.3 Escherichia coli
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.16 culture condition:long-chain fatty acid-grown cell
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.35 20
-
-
 Escherichia coli
2.3.1.16 additional information
-
-
 Escherichia coli
5.1.2.3 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.1.1.35 -76°C, 0.2 M potassium phosphate, pH 6.6, 25% glycerol, 10 mM mercaptoethanol, several months, no loss in activity Escherichia coli
2.3.1.16 -76°C, 0.2 M potassium phosphate, pH 6.6, 25% v/v glycerol, 10 mM 2-mercaptoethanol, stable for months Escherichia coli
5.1.2.3 multienzyme complex which exhibits activities of EC 4.2.1.17, EC 2.3.1.16, EC 5.1.2.3, and EC 5.3.3.3, is stable for months when stored in 0.2 M potassium phosphate, pH 6.6, containing 25% v/v glycerol and 10 mM mercaptoethanol Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+
-
 Escherichia coli acetoacetyl-CoA + NADH
-
 r
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+
-
 Escherichia coli B / ATCC 11303 acetoacetyl-CoA + NADH
-
 r
2.3.1.16 CoA + 3-oxoacyl-CoA
-
 Escherichia coli acyl-CoA + acetyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxoacyl-CoA
-
 Escherichia coli acyl-CoA + acetyl-CoA
-
 r
2.3.1.16 CoA + 3-oxoacyl-CoA
-
 Escherichia coli B / ATCC 11303 acyl-CoA + acetyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxoacyl-CoA
-
 Escherichia coli B / ATCC 11303 acyl-CoA + acetyl-CoA
-
 r
2.3.1.16 CoA + acetoacetyl-CoA
-
 Escherichia coli acetyl-CoA + acetyl-CoA
-
 r
2.3.1.16 additional information highest activities with medium-chain substrates Escherichia coli ?
-
 ?
2.3.1.16 additional information highest activities with medium-chain substrates Escherichia coli B / ATCC 11303 ?
-
 ?
5.1.2.3 D-3-Hydroxydodecanoyl-CoA
-
 Escherichia coli L-3-Hydroxydodecanoyl-CoA
-
 ?
5.1.2.3 D-3-Hydroxydodecanoyl-CoA
-
 Escherichia coli B / ATCC 11303 L-3-Hydroxydodecanoyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.2.3 More multienzyme complex which exhibits activities of EC 4.2.1.17, EC 2.3.1.16, EC 5.1.2.3, and EC 5.3.3.3, 2 * 42000 + 2 * 78000, SDS-PAGE Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.16 6.6
-
 multienzyme complex, -76°C, 25% glycerol v/v, stable for months Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+
-
 Escherichia coli