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Literature summary extracted from

  • Chung, C.H.; Goldberg, A.L.
    Purification and characterization of protease So, a cytoplasmic serine protease in Escherichia coli (1983), J. Bacteriol., 154, 231-238.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.4.21.67 Divalent cations, Mg2+, 5 mM, Ca2+, 5 mM, Mn2+, 5 mM, Co2+, 0.1 mM, stabilizes the enzyme at elevated temperatures Escherichia coli
3.4.21.67 Repeated freezing and thawing has no effect Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.67 1,10-phenanthroline
-
Escherichia coli
3.4.21.67 1,7-phenanthroline
-
Escherichia coli
3.4.21.67 DFP
-
Escherichia coli
3.4.21.67 additional information not: metal chelating agents; tosyllysine chloromethyl ketone Escherichia coli
3.4.21.67 PMSF
-
Escherichia coli
3.4.21.67 tosylphenylalanine chloromethyl ketone
-
Escherichia coli
3.4.21.67 Zn2+
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.67 0.0014
-
casein
-
Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.67 cytoplasm
-
Escherichia coli 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.67 77000
-
2 * 77000, SDS-PAGE Escherichia coli
3.4.21.67 140000
-
E. coli, gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.67 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.67
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.67 additional information
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.4.21.67 -30°C, 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2, 20% v/v glycerol, stable for at least 1 year Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.67 Acid-soluble peptides + H2O
-
Escherichia coli ?
-
?
3.4.21.67 casein + H2O
-
Escherichia coli hydrolyzed casein
-
?
3.4.21.67 Denatured bovine serum albumin + H2O
-
Escherichia coli Hydrolyzed denatured bovine serum albumin
-
?
3.4.21.67 Globin + H2O
-
Escherichia coli Hydrolyzed globin
-
?
3.4.21.67 Glucagon + H2O
-
Escherichia coli Hydrolyzed glucagon
-
?
3.4.21.67 additional information native bovine serum albumin Escherichia coli ?
-
?
3.4.21.67 additional information not: insulin Escherichia coli ?
-
?
3.4.21.67 additional information N-carbobenzoxy-L-alanine-L-alanine-L-leucine 4-nitroanilide Escherichia coli ?
-
?
3.4.21.67 additional information N-benzoyl-L-arginine ethyl ester Escherichia coli ?
-
?
3.4.21.67 additional information N-benzoyl-L-tyrosine ethyl ester Escherichia coli ?
-
?
3.4.21.67 additional information auto alpha fragment of beta-galactosidase Escherichia coli ?
-
?
3.4.21.67 additional information N-acetyl-L-phenylalanine 2-naphthyl ester Escherichia coli ?
-
?
3.4.21.67 additional information N-benzyl-DL-arginine 4-nitroanilide Escherichia coli ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.21.67 dimer 2 * 77000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.67 40
-
10 min, stable Escherichia coli
3.4.21.67 55
-
10 min, complete loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.67 additional information
-
pI: 6.4 Escherichia coli
3.4.21.67 6.5 8
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.67 5 9.5 nearly inactive at pH-values below pH 5.0 and above 9.5 Escherichia coli