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Literature summary extracted from
- Thermomycolin (1976), Methods Enzymol., 45, 415-433.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.21.65 | Autolysis, which is the dominant inactivation process below 50°C, is decreased by the presence of calcium or macromolecules | Malbranchea pulchella |
| 3.4.21.65 | Denaturation by freeze-drying | Malbranchea pulchella |
| 3.4.21.65 | Denaturation by freezing | Malbranchea pulchella |
| 3.4.21.65 | No loss of activity after 12 h of agitation on a reciprocal shaker in the absence of silicone antifoam agent or in the presence of both the antifoam agent and 5% Ficoll | Malbranchea pulchella |
| 3.4.21.65 | Particularly thermostable in the presence of Ca2+ | Malbranchea pulchella |
| 3.4.21.65 | Urea denaturation, 8 M, is markedly inhibited by the binding of a single Ca2+ | Malbranchea pulchella |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.65 | 4-Methylphenylsulfonyl fluorides | - |
Malbranchea pulchella |  |
| 3.4.21.65 | 4-Nitrophenylsulfonyl fluorides | - |
Malbranchea pulchella | |
| 3.4.21.65 | BaCl2 | weak | Malbranchea pulchella | |
| 3.4.21.65 | Carboxybenzoyl halomethyl ketones | L-Phe-CH2Cl and L-Phe-CH2Br | Malbranchea pulchella | |
| 3.4.21.65 | CdCl2 | - |
Malbranchea pulchella | |
| 3.4.21.65 | CoCl2 | - |
Malbranchea pulchella | |
| 3.4.21.65 | CuCl2 | - |
Malbranchea pulchella | |
| 3.4.21.65 | DFP | - |
Malbranchea pulchella | |
| 3.4.21.65 | Egg white trypsin inhibitor | - |
Malbranchea pulchella | |
| 3.4.21.65 | HgCl2 | - |
Malbranchea pulchella | |
| 3.4.21.65 | Lima bean trypsin inhibitor | - |
Malbranchea pulchella | |
| 3.4.21.65 | MgCl2 | - |
Malbranchea pulchella | |
| 3.4.21.65 | MnCl2 | - |
Malbranchea pulchella | |
| 3.4.21.65 | NiCl2 | - |
Malbranchea pulchella | |
| 3.4.21.65 | Phenylmethylsulfonyl fluorides | - |
Malbranchea pulchella | |
| 3.4.21.65 | Soybean trypsin inhibitor | - |
Malbranchea pulchella | |
| 3.4.21.65 | ZnCl2 | - |
Malbranchea pulchella | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.65 | 1.5 | - |
Acetyl-Ala-Ala-Ala methyl ester | - |
Malbranchea pulchella | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.65 | extracellular | - |
Malbranchea pulchella | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.65 | 11000 | 17500 | Malbranchea pulchella var. sulfurea, gel filtration | Malbranchea pulchella |
| 3.4.21.65 | 32000 | - |
Malbranchea pulchella var. sulfurea, sedimentation equilibrium measurement | Malbranchea pulchella |
| 3.4.21.65 | 32700 | - |
Malbranchea pulchella var. sulfurea, amino acid analysis | Malbranchea pulchella |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.65 | Malbranchea pulchella | - |
var. sulfurea | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.65 | - |
Malbranchea pulchella |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.65 | culture medium | - |
Malbranchea pulchella | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.65 | additional information | - |
- |
Malbranchea pulchella |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.21.65 | -20°C | Malbranchea pulchella |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.65 | Acetyl-Ala-Ala-Ala methyl ester + H2O | - |
Malbranchea pulchella | ? | - |
? | |
| 3.4.21.65 | Bovine serum albumin + H2O | - |
Malbranchea pulchella | Hydrolyzed bovine serum albumin | - |
? | |
| 3.4.21.65 | Carboxybenzoyl-Gly 4-nitrophenyl ester + H2O | - |
Malbranchea pulchella | ? | - |
? | |
| 3.4.21.65 | Carboxybenzoyl-L-Ala 4-nitrophenyl ester + H2O | - |
Malbranchea pulchella | ? | - |
? | |
| 3.4.21.65 | Carboxybenzoyl-L-Phe 4-nitrophenyl ester + H2O | - |
Malbranchea pulchella | ? | - |
? | |
| 3.4.21.65 | casein + H2O | - |
Malbranchea pulchella | hydrolyzed casein | - |
? | |
| 3.4.21.65 | Glucagon + H2O | preferential cleavage of Thr5-Phe6, Ser8-Asp9, Lys12-Tyr13, Asp15-Ser16, Ser16-Arg17 and Arg17-Arg18, no amino acids observed in the digest | Malbranchea pulchella | Hydrolyzed glucagon | no amino acids observed in the digest | ? | |
| 3.4.21.65 | additional information | slow release of peptides from native collagen | Malbranchea pulchella | ? | - |
? | |
| 3.4.21.65 | additional information | active site sequence: Leu-Ser-(Gly)-Thr-Ser-Met | Malbranchea pulchella | ? | - |
? | |
| 3.4.21.65 | additional information | specificity towards small synthetic ester substrates: preference in decreasing order for L-Ala, L-Tyr, L-Phe, Gly, L-Leu, L-Trp, L-Val, L-Lys, L-Pro on the carboxyl side of the bond hydrolyzed | Malbranchea pulchella | ? | - |
? | |
| 3.4.21.65 | additional information | no keratinase activity | Malbranchea pulchella | ? | - |
? | |
| 3.4.21.65 | Oxidized insulin A-chain + H2O | no amino acids observed in the digest | Malbranchea pulchella | Hydrolyzed oxidized insulin A-chain | no amino acids observed in the digest | ? | |
| 3.4.21.65 | Oxidized insulin B-chain + H2O | no amino acids observed in the digest | Malbranchea pulchella | Hydrolyzed oxidized insulin B-chain | no amino acids observed in the digest | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.65 | ? | - |
Malbranchea pulchella |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.65 | additional information | - |
significant thermostability, especially in the presence of Ca2+ | Malbranchea pulchella |
| 3.4.21.65 | 70 | - |
maximal thermostability 10 mM Ca2+ | Malbranchea pulchella |
| 3.4.21.65 | 73 | - |
t1/2: 7.5 min without Ca2+ | Malbranchea pulchella |
| 3.4.21.65 | 73 | - |
110 min with 10 mM Ca2+ | Malbranchea pulchella |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.65 | additional information | - |
additional information | - |
Malbranchea pulchella |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.65 | additional information | - |
pI: 6.0 | Malbranchea pulchella |
| 3.4.21.65 | 8.5 | - |
casein | Malbranchea pulchella |
| 3.4.21.65 | 8.5 | - |
bovine serum albumin | Malbranchea pulchella |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.65 | 3.5 | - |
rapid and irreversible denaturation below | Malbranchea pulchella |
| 3.4.21.65 | 4.5 | 10.5 | 30°C, 2 h, stable | Malbranchea pulchella |
| 3.4.21.65 | 6 | 9.6 | 30°C, 20 h, stable | Malbranchea pulchella |
| 3.4.21.65 | 8.5 | - |
optimum pH for stability | Malbranchea pulchella |
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