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Literature summary extracted from

  • Kamal, M.; Höög, J.O.; Kaiser, R.; Shafqat, J.; Razzaki, T.; Zaidi, Z.H.; Jörnvall, H.
    Isolation, characterization and structure of subtilisin from a thermostable Bacillus subtilis isolate (1995), FEBS Lett., 374, 363-366.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.62
-
 Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.62 3,4-dichloroisocoumarin
-
 Bacillus subtilis
3.4.21.62 phenylmethanesulfonyl fluoride
-
 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus subtilis
-
 thermostable soil isolate from the Tharparkar desert of Pakistan
-
3.4.21.62 Bacillus subtilis RT-5
-
 thermostable soil isolate from the Tharparkar desert of Pakistan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.62
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
-
 Bacillus subtilis succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.62 succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
-
 Bacillus subtilis RT-5 succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.62 50
-
 3 h, 30% loss of activity, thermostable strain Bacillus subtilis