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Literature summary extracted from

  • Markland, F.S.; Smith, E.L.
    Subtilisins: primary structure, chemical and physical properties (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 3, 561-608.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.62 4-(4'-Aminophenylazo)phenylarsonic acid
-
 Bacillus sp. (in: Bacteria)
3.4.21.62 p-Nitrophenylarsonate
-
 Bacillus sp. (in: Bacteria)
3.4.21.62 Phenylarsonate
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.62 additional information
-
 additional information
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.62 additional information
-
 subtilisin Carlsberg Bacillus sp. (in: Bacteria)
3.4.21.62 additional information
-
 subtilisin Carlsberg Bacillus amyloliquefaciens
3.4.21.62 additional information
-
 subtilisin Novo Bacillus sp. (in: Bacteria)
3.4.21.62 additional information
-
 subtilisin Novo Bacillus amyloliquefaciens
3.4.21.62 additional information
-
 subtilisin amylosacchariticus Bacillus sp. (in: Bacteria)
3.4.21.62 additional information
-
 subtilisin amylosacchariticus Bacillus amyloliquefaciens
3.4.21.62 additional information
-
 amino acid composition of: subtilisin BPN' Bacillus sp. (in: Bacteria)
3.4.21.62 additional information
-
 amino acid composition of: subtilisin BPN' Bacillus amyloliquefaciens
3.4.21.62 27000
-
 subtilisin BPN', sedimentation equilibrium measurement Bacillus sp. (in: Bacteria)
3.4.21.62 27280
-
 subtilisin Carlsberg, amino acid sequence Bacillus sp. (in: Bacteria)
3.4.21.62 27400
-
 subtilisin Carlsberg, sedimentation equilibrium measurement Bacillus sp. (in: Bacteria)
3.4.21.62 27670
-
 Bacillus sp., subtilisin amylosacchariticus, amino acid sequence Bacillus sp. (in: Bacteria)
3.4.21.62 28000
-
 Bacillus sp., subtilisin amylosacchariticus, sedimentation equilibrium measurement Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus amyloliquefaciens
-
-
-
3.4.21.62 Bacillus sp. (in: Bacteria)
-
 subtilisin amylosacchariticus
-
3.4.21.62 Bacillus sp. (in: Bacteria)
-
 subtilisin DY
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.62 Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. Mechanism Bacillus sp. (in: Bacteria)
a
3.4.21.62 Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. Mechanism Bacillus amyloliquefaciens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 Benzoyl-Arg ethyl ester + H2O subtilisin Carlsberg, subtilisin Novo Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 Benzoyl-Arg ethyl ester + H2O subtilisin BPN', subtilisin Amylosacchariticus Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 Carboxybenzoyl-Gly-Ala-NH2 + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) Carboxybenzoyl-Gly-Ala + NH3 subtilisin BPN' ?
3.4.21.62 Carboxybenzoyl-Gly-Leu-NH2 + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) Carboxybenzoyl-Gly-Leu + NH3 subtilisin BPN' ?
3.4.21.62 Carboxybenzoyl-Gly-Pro-Leu-Gly-Pro-OH + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) Carboxybenzoyl-Gly-Pro-Leu + Gly-Pro-OH subtilisin BPN' ?
3.4.21.62 additional information reactions catalyzed: 1. peptide bond hydrolysis, 2. ester bond hydrolysis, 3. transesterification, 4. transpeptidation Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 additional information reactions catalyzed: 1. peptide bond hydrolysis, 2. ester bond hydrolysis, 3. transesterification, 4. transpeptidation Bacillus amyloliquefaciens ?
-
 ?
3.4.21.62 N-Acetyl-Ala methyl ester + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Gly ethyl ester + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-L-norvaline ethyl ester + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Leu methyl ester + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Lys methyl ester + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Phe ethyl ester + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Phe methyl ester + H2O subtilisin Amylosacchariticus Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Phe methyl ester + H2O subtilisin Carlsberg, subtilisin Novo Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Trp ethyl ester + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Trp methyl ester + H2O subtilisin Amylosacchariticus Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Trp methyl ester + H2O subtilisin Carlsberg, subtilisin Novo Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Tyr ethyl ester + H2O subtilisin Carlsberg, subtilisin Novo Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Tyr ethyl ester + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Tyr methyl ester + H2O subtilisin Amylosacchariticus Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Tyr methyl ester + H2O subtilisin Carlsberg, subtilisin Novo Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Val methyl ester + H2O subtilisin Carlsberg, subtilisin Novo Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-Acetyl-Val methyl ester + H2O subtilisin BPN' Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 N-tosyl-L-Arg methyl ester + H2O subtilisin Carlsberg, subtilisin Novo, subtilisin Amylosacchariticus Bacillus sp. (in: Bacteria) N-tosyl-L-Arg + methanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 More species variants include subtilisin BPN' (also subtilisin B, subtilopeptidase B, subtilopeptidase C, Nagarse, Nagarse proteinase, subtilisin Novo, bacterial proteinase Novo) and subtilisin Carlsberg (subtilisin A, subtilopeptidase A, alcalase Novo), similar enzymes are produced by various Bacillus subtilis strains and other Bacillus species Bacillus sp. (in: Bacteria)
3.4.21.62 More species variants include subtilisin BPN' (also subtilisin B, subtilopeptidase B, subtilopeptidase C, Nagarse, Nagarse proteinase, subtilisin Novo, bacterial proteinase Novo) and subtilisin Carlsberg (subtilisin A, subtilopeptidase A, alcalase Novo), similar enzymes are produced by various Bacillus subtilis strains and other Bacillus species Bacillus amyloliquefaciens
3.4.21.62 Subtilisin DY
-
 Bacillus sp. (in: Bacteria)
3.4.21.62 Subtilisin DY
-
 Bacillus amyloliquefaciens