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Literature summary extracted from

  • Braganza, V.J.; Simmons, W.H.
    Tryptase from rat skin: purification and properties (1991), Biochemistry, 30, 4997-5007.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.59 stable to repeated freeze-thaw cycles Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.59 4-Methylumbelliferyl 4-guanidinobenzoate
-
Rattus norvegicus
3.4.21.59 4-nitrophenyl 4-guanidinobenzoate
-
Rattus norvegicus
3.4.21.59 alpha1-antitrypsin not Rattus norvegicus
3.4.21.59 antipain
-
Rattus norvegicus
3.4.21.59 Aprotinin
-
Rattus norvegicus
3.4.21.59 diisopropyl fluorophosphate
-
Rattus norvegicus
3.4.21.59 HgCl2
-
Rattus norvegicus
3.4.21.59 leupeptin
-
Rattus norvegicus
3.4.21.59 additional information lima bean trypsin inhibitor; ovomucoid Rattus norvegicus
3.4.21.59 N-tosyl-L-Lys chloromethyl ketone
-
Rattus norvegicus
3.4.21.59 Soybean trypsin inhibitor not Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.59 145000
-
rat, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.59 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.59 glycoprotein treatment of the enzyme with N-glycosidase decreases the subunit MW by 4000 Rattus norvegicus
3.4.21.59 glycoprotein putative glycosylation site at residue 21 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.59 multiple isoelectric forms Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.59 skin
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.59 additional information
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.4.21.59 25°C, stable for at least 8 days Rattus norvegicus
3.4.21.59 4°C, stable for at least 6 months in buffer containing NaCl, rapid loss of activity without addition of NaCl Rattus norvegicus
3.4.21.59 NaCl, 1 M, necessary for long term storage Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.59 additional information specificity overview: peptide 4-nitroanilides Rattus norvegicus ?
-
?
3.4.21.59 additional information not: casein, hemoglobin, azocoll Rattus norvegicus ?
-
?
3.4.21.59 N-Benzoyl-Arg ethyl ester + H2O
-
Rattus norvegicus ?
-
?
3.4.21.59 neurotensin + H2O
-
Rattus norvegicus ?
-
?
3.4.21.59 Oxidized B-chain of insulin + H2O cleavage at Arg8-Gly9-NH2, Arg8-Arg9- and Arg22-Gly23-bonds Rattus norvegicus ?
-
?
3.4.21.59 [Arg8]vasopressin + H2O
-
Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.21.59 ? x * 30000-34000, rat Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.59 additional information
-
presence of 1 M NaCl improves thermal stability, heparin does not stabilize the enzyme against thermal denaturation Rattus norvegicus
3.4.21.59 25
-
pH 7.5, 30 min, stable up to Rattus norvegicus
3.4.21.59 30
-
pH 7.5, 30 min, 50% loss of activity Rattus norvegicus
3.4.21.59 35
-
pH 7.5, 30 min, complete loss of activity at 35°C or above Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.59 7.5 8
-
Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.59 6 8.5 no activity below pH 6 or above pH 8.5 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.59 3.5 7.5 25°C, 2 h, relatively stable in this range, rapid loss of activity below and above Rattus norvegicus