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Literature summary extracted from

  • Harvima, I.T.; Schechter, N.M.; Harvima, R.J.; Fräki, J.E.
    Human skin tryptase: purification, partial characterization and comparison with human lung tryptase (1988), Biochim. Biophys. Acta, 957, 71-80.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.59 addition of protein to the tryptase-heparin mixture is needed to keep the enzyme stable Homo sapiens
3.4.21.59 heparin glycosaminoglycan partially stabilizes the tryptase Homo sapiens
3.4.21.59 In low-salt buffer the enzyme is labile, at least 1.4 M KCl is needed to keep the enzyme stable if incubated at 37°C for 30 min Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.59 benzamidine
-
Homo sapiens
3.4.21.59 diisopropyl fluorophosphate
-
Homo sapiens
3.4.21.59 leupeptin
-
Homo sapiens
3.4.21.59 N-tosyl-L-Phe chloromethyl ketone
-
Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.59 34000
-
x * 34000 + x * 38000, human, SDS-PAGE Homo sapiens
3.4.21.59 38000
-
x * 34000 + x * 38000, human, SDS-PAGE Homo sapiens
3.4.21.59 120000
-
human, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.59 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.59
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.59 lung
-
Homo sapiens
-
3.4.21.59 skin
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.59 additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.59 Benzyloxycarbonyl-Arg 4-nitroanilide + H2O
-
Homo sapiens Benzyloxycarbonyl-Arg + 4-nitroaniline
-
?
3.4.21.59 Benzyloxycarbonyl-Gly-Arg 4-trifluoromethylcoumarin 7-amide + H2O
-
Homo sapiens Benzyloxycarbonyl-Gly-Arg + 7-amino-4-trifluoromethylcoumarin
-
?
3.4.21.59 Benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide + H2O
-
Homo sapiens Benzyloxycarbonyl-Gly-Pro-Arg + 4-nitroaniline
-
?
3.4.21.59 Benzyloxycarbonyl-Lys-Arg 4-trifluoromethylcoumarin 7-amide + H2O
-
Homo sapiens Benzyloxycarbonyl-Lys-Arg + 7-amino-4-trifluoromethylcoumarin
-
?

Subunits

EC Number Subunits Comment Organism
3.4.21.59 tetramer x * 34000 + x * 38000, human, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.59 5.35
-
benzyloxycarbonyl-Arg 4-nitroanilide skin enzyme Homo sapiens
3.4.21.59 5.52
-
benzyloxycarbonyl-Arg 4-nitroanilide lung enzyme Homo sapiens
3.4.21.59 45.7
-
Benzyloxycarbonyl-Lys-Arg 4-trifluoromethylcoumarin 7-amide skin and lung enzyme Homo sapiens
3.4.21.59 104
-
Benzyloxycarbonyl-Gly-Arg 4-trifluoromethylcoumarin 7-amide skin enzyme Homo sapiens
3.4.21.59 107
-
Benzyloxycarbonyl-Gly-Arg 4-trifluoromethylcoumarin 7-amide lung enzyme Homo sapiens
3.4.21.59 551
-
Benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide skin enzyme Homo sapiens
3.4.21.59 553
-
Benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide lung enzyme Homo sapiens