Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Aducci, P.; Ascenzi, P.; Pierini, M.; Ballio, A.
    Purification and characterization of Leu-proteinase, the leucine specific serine proteinase from spinach (Spinacia oleracea L.) leaves (1986), Plant Physiol., 81, 812-816.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.57 diisopropyl fluorophosphate
-
 Spinacia oleracea
3.4.21.57 Nalpha-tosyl-L-Leu chloromethyl ketone
-
 Spinacia oleracea
3.4.21.57 phenylmethylsulfonyl fluoride
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.57 0.05
-
 Nalpha-benzoyl-Leu 4-nitroanilide
-
 Spinacia oleracea
3.4.21.57 0.18
-
 Nalpha-benzoyl-Val 4-nitroanilide
-
 Spinacia oleracea
3.4.21.57 0.25
-
 Nalpha-benzoyl-Ala 4-nitroanilide
-
 Spinacia oleracea
3.4.21.57 0.45
-
 Nalpha-benzoyl-Lys 4-nitroanilide
-
 Spinacia oleracea
3.4.21.57 0.47
-
 Nalpha-benzoyl-L-Arg-4-nitroanilide
-
 Spinacia oleracea
3.4.21.57 0.63
-
 azocasein
-
 Spinacia oleracea
3.4.21.57 0.89
-
 Nalpha-benzoyl-Gly 4-nitroanilide
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.57 60000
-
 Spinacia oleracea, gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.57 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.57 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.57 4.5
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.57 azocasein + H2O
-
 Spinacia oleracea ?
-
 ?
3.4.21.57 Azocoll + H2O
-
 Spinacia oleracea ?
-
 ?
3.4.21.57 Nalpha-benzoyl-Gly 4-nitroanilide + H2O
-
 Spinacia oleracea Nalpha-benzoyl-Gly + 4-nitroaniline
-
 ?
3.4.21.57 Nalpha-benzoyl-L-Ala 4-nitroanilide + H2O
-
 Spinacia oleracea Nalpha-benzoyl-L-Ala + 4-nitroaniline
-
 ?
3.4.21.57 Nalpha-benzoyl-L-Arg 4-nitroanilide + H2O
-
 Spinacia oleracea Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
 ?
3.4.21.57 Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O
-
 Spinacia oleracea Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
 ?
3.4.21.57 Nalpha-benzoyl-L-Leu 4-nitroanilide + H2O
-
 Spinacia oleracea Nalpha-benzoyl-L-Leu + 4-nitroaniline
-
 ?
3.4.21.57 Nalpha-benzoyl-L-Lys 4-nitroanilide + H2O
-
 Spinacia oleracea Nalpha-benzoyl-L-Lys + 4-nitroaniline
-
 ?
3.4.21.57 Nalpha-benzoyl-Val 4-nitroanilide + H2O
-
 Spinacia oleracea Nalpha-benzoyl-Val + 4-nitroaniline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.57 1.2
-
 Nalpha-benzoyl-Gly 4-nitroanilide
-
 Spinacia oleracea
3.4.21.57 1.8
-
 Nalpha-benzoyl-L-Lys 4-nitroanilide
-
 Spinacia oleracea
3.4.21.57 2.1
-
 Nalpha-benzoyl-L-Arg-4-nitroanilide
-
 Spinacia oleracea
3.4.21.57 4.9
-
 Nalpha-benzoyl-L-Ala 4-nitroanilide
-
 Spinacia oleracea
3.4.21.57 6.1
-
 Nalpha-benzoyl-Val 4-nitroanilide
-
 Spinacia oleracea
3.4.21.57 21.3
-
 Nalpha-benzoyl-Leu 4-nitroanilide
-
 Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.57 additional information
-
 pI: 4.8 Spinacia oleracea