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Literature summary extracted from

  • Eng, F.J.; Novikova, E.G.; Kuroki, K.; Ganem, D.; Fricker, L.D.
    gp180, A protein that binds duck hepatitis B virus particles, has metallocarboxypeptidase D-like enzymatic activities (1998), J. Biol. Chem., 273, 8382-8388.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.22 expression in Sf9 cells, using a baculovirus expression system Anas platyrhynchos

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.22 1,10-phenanthroline
-
 Anas platyrhynchos
3.4.17.22 Guanidinoethylmercaptosuccinic acid i.e. GEMSA Anas platyrhynchos
3.4.17.22 leupeptin not inhibitory Anas platyrhynchos
3.4.17.22 additional information not inhibitory: iodoacetate, phenylmethylsulfonylfluoride, benzamidine, N-tosyl-L-Phe chloromethyl ketone, N- tosyl-L-Lys chloromethyl ketone Anas platyrhynchos
3.4.17.22 p-chloromercuriphenylsulfonate
-
 Anas platyrhynchos

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.22 0.012
-
 dansyl-Phe-Ala-Arg enzyme form gp170 Anas platyrhynchos
3.4.17.22 0.017
-
 Dansyl-Pro-Ala-Arg enzyme form gp170 Anas platyrhynchos
3.4.17.22 0.021
-
 dansyl-Phe-Phe-Arg enzyme form gp170 Anas platyrhynchos
3.4.17.22 0.054
-
 Dansyl-Pro-Ala-Arg dansyl-Pro-Ala-Arg, enzyme form gp180 Anas platyrhynchos
3.4.17.22 0.063
-
 dansyl-Phe-Ala-Arg dansyl-Phe-Ala-Arg, enzyme form gp180 Anas platyrhynchos

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.22 Cd Cl2 activation of enzyme form gp170, inhibition of gp180 Anas platyrhynchos
3.4.17.22 CoCl2 1 mM, activation Anas platyrhynchos
3.4.17.22 additional information no activation by CaCl2, MnCl2 Anas platyrhynchos
3.4.17.22 ZnCl2 1 mM, activation Anas platyrhynchos

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.22 Anas platyrhynchos
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.22 enzyme form gp170, lacking the C-terminal transmembrane domain Anas platyrhynchos

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.22 Dansyl-Phe-Ala-Arg + H2O
-
 Anas platyrhynchos Dansyl-Phe-Ala + Arg
-
 ?
3.4.17.22 Dansyl-Phe-Phe-Arg + H2O
-
 Anas platyrhynchos Dansyl-Phe-Phe + Arg
-
 ?
3.4.17.22 Dansyl-Pro-Ala-Arg + H2O
-
 Anas platyrhynchos Dansyl-Pro-Ala + Arg
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.22 5.9
-
 dansyl-Phe-Phe-Arg enzyme form gp180 Anas platyrhynchos
3.4.17.22 17
-
 dansyl-Phe-Phe-Arg enzyme form gp170 Anas platyrhynchos
3.4.17.22 22
-
 Dansyl-Pro-Ala-Arg enzyme form gp180 Anas platyrhynchos
3.4.17.22 51
-
 dansyl-Phe-Ala-Arg enzyme form gp180 Anas platyrhynchos
3.4.17.22 57
-
 dansyl-Phe-Ala-Arg enzyme form gp170 Anas platyrhynchos

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.22 5.5 6.5
-
 Anas platyrhynchos